RESUMO
Analysis of Reg1a gene expression in rat gastric mucosa under development and healing of stress-induced lesions was carried out. Increased expression of Reg1a was observed after 1 hour of stressor impact - 2,1 times, and achieved the maximum level expression after 3 hours of stress exposure - 3,5 times, that occurred on the background of lipid peroxidation intensification and antioxidant system dysfunction. The sharp decrease in 1,6 and 2 times of Reg1a gene expression was shown in 12 and 24 hours respectively after termination of the stressor action. Analysis of Gast gene expression did not confirm that gastrin stimulated Reg1a expression in gastric mucosa under water immersion restraint stress. The positive correlation between Reg1a and Tgfb1 genes expression was determined in the dynamics of stress-induced gastric lesions' development and healing, which may indicate the involvement of Tgfb1 to acceleration of lesion's healing.
Assuntos
Mucosa Gástrica/metabolismo , Gastrinas/genética , Litostatina/genética , Úlcera Gástrica/genética , Estresse Psicológico/genética , Fator de Crescimento Transformador beta1/genética , Animais , Animais não Endogâmicos , Mucosa Gástrica/patologia , Gastrinas/metabolismo , Regulação da Expressão Gênica , Imersão/fisiopatologia , Imobilização/métodos , Peroxidação de Lipídeos , Litostatina/metabolismo , Masculino , Malondialdeído/sangue , Estresse Oxidativo , Ratos , Recuperação de Função Fisiológica/fisiologia , Estômago/patologia , Úlcera Gástrica/etiologia , Úlcera Gástrica/metabolismo , Úlcera Gástrica/patologia , Estresse Psicológico/complicações , Estresse Psicológico/metabolismo , Estresse Psicológico/patologia , Fator de Crescimento Transformador beta1/metabolismo , ÁguaRESUMO
Expression of Tlr2, Muc2, Defa genes in rat duodenal upon long-term gastric hypacidity (hypochlorhydria) and with multiprobiotic correction was investigated. Increasing of Tlr2, Muc2, Defa expression on the background of the intensification of lipid peroxidation in rat duodenal villus and crypt epithelial cells upon gastric hypoacidity was shown. The mRNAs patterns of genes mentioned above were shown to decrease or approach to control values as well as the level of lipid peroxidation products under the treatment of hypoacidic rats with the multiprobiotic. The data obtained may indicate involvement of genes Tlr2, Muc2, Defa in development of inflammation in the duodenum due to dysbiotic changes under conditions of prolonged hypochlorhydria.
Assuntos
Acloridria/terapia , Disbiose/terapia , Mucina-2/genética , Probióticos/farmacologia , Receptor 2 Toll-Like/genética , alfa-Defensinas/genética , Acloridria/induzido quimicamente , Acloridria/genética , Acloridria/microbiologia , Animais , Duodeno/efeitos dos fármacos , Duodeno/metabolismo , Duodeno/microbiologia , Disbiose/induzido quimicamente , Disbiose/genética , Disbiose/microbiologia , Regulação da Expressão Gênica , Concentração de Íons de Hidrogênio , Masculino , Mucina-2/metabolismo , Omeprazol/administração & dosagem , Inibidores da Bomba de Prótons/administração & dosagem , Ratos , Estômago/efeitos dos fármacos , Estômago/microbiologia , Receptor 2 Toll-Like/metabolismo , alfa-Defensinas/metabolismoRESUMO
Changes in redox status of gastric mucosa cells are the main pathogenic factor of gastric erosion and gastric ulcer development. Pro-oxidants can affect cell transcription activity via changes in redox-sensitive transcription factors. Egr-1 and Sp-1 may regulate the transcription of genes that are associated with the pathogenesis of gastric ulcer (growthfactors, cell cycle regulators, etc.). The aim of the present study was to reveal the possible involvement of zinc-finger transcriptionfactors Egr-1 & Sp-1 in the molecular mechanisms underlying gastric lesions caused by aspirin administration and stress. Gastric ulcer was induced in male rats (180-220 g) by immobilization stress combined with water-immersion (IMO-WI) or aspirin gavage (10 mg/100 g). The rats were euthanized 20 min, 1 hour, or 3 hours following the ulcerogenic factor exposure. Protein expression was determined by Western blot analysis and RT-PCR; levels of SH-groups of proteins were determined by method of Ellman et al. Development of gastric ulcer lesions was associated with twofold (P < 0.05) decrease in concentration of protein SH-groups in the rat gastric mucosa. These changes were accompanied by significant (P < 0.05) increase in the expression of Egr-1 mRNA and protein in both gastric ulcer models, and the changes in IMO-WI were more profound. Increased levels of Egr-1 were associated with the decrease in SpI protein levels. We showed for the first time the competitive interaction between redox-sensitive transcription factors Egr-1 and Sp1 in the early phases of gastric ulcer development, which might facilitate inducible transcriptional activity of Egr-1 at the expense of reduction in Sp1 activity.
Assuntos
Proteína 1 de Resposta de Crescimento Precoce/genética , Mucosa Gástrica/metabolismo , RNA Mensageiro/genética , Fator de Transcrição Sp1/genética , Úlcera Gástrica/metabolismo , Estresse Psicológico/metabolismo , Animais , Aspirina/efeitos adversos , Proteína 1 de Resposta de Crescimento Precoce/metabolismo , Mucosa Gástrica/patologia , Regulação da Expressão Gênica , Imobilização , Masculino , Oxirredução , RNA Mensageiro/metabolismo , Ratos , Transdução de Sinais , Fator de Transcrição Sp1/metabolismo , Úlcera Gástrica/etiologia , Úlcera Gástrica/genética , Úlcera Gástrica/patologia , Estresse Psicológico/complicações , Estresse Psicológico/genética , Estresse Psicológico/patologia , Compostos de Sulfidrila/metabolismo , Fatores de Tempo , Transcrição GênicaRESUMO
RAPD analysis was used to examine the extent of genetic polymorphism in two populations of Gentoo penguin (Pygoscelis papua) from Antarctic Islands (Petermann and Livingston). The chosen two of three 10 mer oligonucleotide primers accordingly to preliminary results showed different levels of polymorphism in Gentoo penguins at Petermann Island (from 23.53 to 42.86%) and Livingston Island (from 52.94 to 57.14%). Nei's similarity coefficients were in range from 0.5606 (when Gentoo genome profiles were compared with RAPD profiles of two related penguin species: Pygoscelis adeliae (Adelie) and Pygoscelis antarctica (Chinstrep)) to 0.9281 among observed Gentoo penguin populations. Nei's distances values ranged from 0.0746 to 0.5787 among the populations and species. The obtained results will be used for further estimation of genetic diversity of Gentoo penguins and determination of their taxonomic status.
