RESUMO
The effect of 10 days of interval hypoxic training (IHT) on the mRNA expression and protein content of antioxidant enzymes (superoxide dismutase, catalase and glutathione-S-transferase) in leukocytes of children with bronchial asthma (BA) was investigated. It was shown that after sessions of IHT the mRNA expression of superoxide dismutase decreased by 32.5% (P < 0,05), but the level of protein was unchanged. The level of catalase gene mRNA and protein content in leukocytes after IHT increased by 67% and 13% accordingly. We detected a 90% increase in glutathione S-transferase level following IHT.
Assuntos
Adaptação Fisiológica/fisiologia , Antioxidantes/metabolismo , Asma/terapia , Hipóxia/fisiopatologia , RNA Mensageiro/biossíntese , Adolescente , Asma/sangue , Asma/enzimologia , Asma/fisiopatologia , Catalase/biossíntese , Catalase/genética , Criança , Feminino , Expressão Gênica , Glutationa Transferase/biossíntese , Glutationa Transferase/genética , Humanos , Hipóxia/enzimologia , Leucócitos/enzimologia , Masculino , RNA Mensageiro/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Superóxido Dismutase/biossíntese , Superóxido Dismutase/genética , Resultado do TratamentoRESUMO
The investigation was carried out on 45 men aged 43.1 +/- 2.9 years which were team members of the Antarctic station Academician Vernadsky. Among participants of expedition, we revealed 22% of winterers with the heterozygotic genotype C/T HIF-1alpha. It was shown that the persons with a heterozygotic genotype were more predisposed to the latent hypoxia development during long stay in Antarctica.
Assuntos
Adaptação Fisiológica/genética , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Hipóxia/genética , Polimorfismo de Nucleotídeo Único , Adulto , Regiões Antárticas , Gasometria , Teste de Esforço , Hemodinâmica/genética , Heterozigoto , Humanos , Hipóxia/fisiopatologia , Fenômenos Fisiológicos Respiratórios , Fatores de TempoRESUMO
The majority of the adaptation processes to hypoxia is based on transcriptional regulation by hypoxia-inducible factors--HIFs. Recently the allele polymorphism of oxygen-dependent degradation domain of HIF-lalpha has been described. It consists in the replacement of cytosine for thymine in 1772 location (C1772-->T). The physiological significance of such replacement is obscure. In the investigation of 26 healthy elderly subjects (58.5 +/- 0.7 yr) we tried to verify whether HIF-lalpha polymorphism in exon 12 may identify individual features of adaptation to intermittent hypoxia training (IHT) (isocapnic hypoxic rebreathing technique, 5-min sessions with 5 min rest intervals, 3 times daily during 10 days). The distribution of HIF-l alpha genotypes for C1772-->T were studied by using the polymerase chain reaction and restriction analysis. We detected that all subjects from the group had C/C genotype. Meanwhile, the broad spectrum of adaptive reactions to IHT was observed, from the best adaptation up to disadaptation. IHT enhanced HVRs in the range of 167% - 5%, blood malon dialdehyde content varied from a decrease by 46% up to an increase by 88%, the changes of superoxide dismutase activity varied from +64% to -56% etc. These results suggest that the C1772-->T polymorphism in HIF- 1alpha does not contribute to individual peculiarities of adaptation to IHT. Because the activity of HIF-lalpha is regulated by multiple steps including the transcriptional level, the effect of the polymorphism in enother exons on the adaptive reactions remains to be elucidated.
