Cell cycle, melanin contents and apoptosis processes in B16 and Cloudman S91 mouse melanoma cells after exposure to cytostatic drugs.

Free radicals are generated in cells during many metabolic processes and eliminated from an organism by complex enzymatic and nonenzymatic systems. Many chemical compounds--among others melanin, reveal antioxidative properties. In this work the influence of some cytostatic drugs (at EC50) on melanin content and on the apoptotic processes in mouse melanoma B16 and Cloudman S91 cells in vitro were investigated. The cells were incubated with adriblastin, actinomycin D, cytosine arabinoside, cisplatin, dacarbazine and vincristine. The number of viable mouse melanoma B16 and Cloudman S91 cells was estimated by flow cytometry analysis and melanin content in colorimetric assays. Apoptotic cells were detected using the annexin V-FITC test. The majority of tested cytostatic drugs caused an increase of melanin content in the cells of both melanoma lines, except cisplatin and dacarbazine in the case of B16 cells and dacarbazine in the case of Cloudman S91. Adriblastin, actinomycin D and vincristine evoked apoptosis in the both tested cell lines. Slight increase of melanin content in melanoma cells can be a cell answer to free radicals generation by some cytostatic drugs like adriblastin, actinomycin D and vincristine.

[Cell culture, tissue engineering and regenerative medicine. Part I]. / Hodowla komórek, inzynieria tkankowa i medycyna regeneracyjna. Czesct I.

Tissue engineering is an interdisciplinary field that applies the principles and methods of engineering and the cell culture toward the development of biomaterials that restore, maintain or improve tissue function. The amalgamation of engineering and medicine has interested many scientists for at last two hundred years. What was the goal of cell culture? First, for progress in life sciences achievement and subsequent for virology and toxicology development. In vitro studies are done because of many problems with carrying out animal experiments. In this work the authors present the attempts of physicians, anatomopathologists, embryologists and biologists which contributed to fast development of new area in medicine--tissue engineering.

[Tissue engineering yesterday and today. Part II]. / Hodowla komórek, inzynieria tkankowa i medycyna regeneracyjna. Czesc II.

Science and technology is always an important component of human culture. Science directs technological innovation and technology accelerates the progress of science. Increasing number of achievements in tissue engineering domain make possible ex vivo maintaining not only the isolated cells, but also tissues and organs. Tissue engineering powerfully expanding from over hundred years, contribute to the development of genetics, transplantology, oncological diagnostics, pharmacology, toxicology and many other medical sciences. Presented paper summarizes the scientists' accomplishments in the field of tissue engineering which were noted in the second half of twentieth century.

B16 and cloudman S91 mouse melanoma cells susceptibility to apoptosis after dacarbazine treatment.

Considering the necessity of an individual choice of cytostatic drugs for patients with cancer disease and tumor cells' resistance to these compounds, their ability to induction of apoptosis should be investigated. The aim of this study was to determine the influence of dacarbazine (DTIC) on morphology and kinetics of proliferation of B16 and Cloudman S91 cells. It is important to determine the kind of death induced by the DTIC and the effect of a specific concentration. The evaluation of apoptosis and necrosis in these two mouse melanoma cell lines in vitro was performed. Induction of apoptosis was estimated in annexin V binding assay by flow cytometry. DNA content and cell cycle phases were determined by propidium iodide staining. DTIC induced morphological changes typical for apoptosis and necrosis in both cell lines. DTIC caused cell cycle arrest in S and G2/M phase of both cell lines which showed hypertetraploidy. The highest induction of apoptosis was observed in DTIC concentration of 200 microg/mL for B16 cells (11%) and 100 microg/mL for apoptosis Cloudman S91 cells (22.2%). Higher doses of DTIC caused intensification of necrotic process. The B16 melanoma cells are more sensitive to DTIC than the Cloudman S91 cells, however more intensive apoptotic process was detected in Cloudman S91 cells already at lower concentration of DTIC.