1.
Biokhimiia
; 48(7): 1195-202, 1983 Jul.
Artigo
em Russo
| MEDLINE
| ID: mdl-6577914
RESUMO
A purification technique for a large scale production of crystalline Mo-Fe protein of nitrogenase from Azotobacter vinelandii and of its fragment, Fe-Mo-cofactor (Fe-Mo-co) in argon atmosphere has been elaborated. The novel fluorimetric method of thiol compounds analysis has been proposed for identification of Fe-Mo-co thiol ligands; this procedure allows the determination of concentration and class of thiol compounds and of the distance between sulphur atoms in the case of dithiols. The use of this method for an analysis of Fe-Mo-co has demonstrated that it contains a thiomolybdate fragment and two atoms of inorganic sulphur. Organic thiol as a Fe ligand has not been revealed.
Assuntos
Azotobacter/enzimologia , Ferredoxinas/metabolismo , Molibdoferredoxina/metabolismo , Nitrogenase/metabolismo , Compostos de Sulfidrila/análise , Cinética , Espectrometria de Fluorescência/métodos
2.
Radiobiologiia
; 21(4): 563-7, 1981.
Artigo
em Russo
| MEDLINE
| ID: mdl-7302163