RESUMO
Identification of two novel HLA alleles in Russian bone marrow donors.
Assuntos
Alelos , Doadores de Tecidos , Humanos , Federação Russa , Antígeno HLA-A2/genética , Antígeno HLA-A2/imunologia , Transplante de Medula Óssea , Antígeno HLA-B40/genética , Antígeno HLA-B40/imunologia , Éxons , Teste de Histocompatibilidade , Medula Óssea , Análise de Sequência de DNARESUMO
Novel HLA-B*55:01:31, HLA-C*07:1113 alleles and confirmatory HLA-C*12:392 allele were detected during the HLA typing process.
Assuntos
Genes MHC Classe I , Antígenos HLA-C , Humanos , Alelos , Antígenos HLA-C/genética , Antígenos HLA-B/genética , Teste de HistocompatibilidadeRESUMO
We identified two novel HLA alleles by NGS, HLA-B*51:395 and HLA-DQB1*06:478.
Assuntos
Antígenos HLA-B , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Alelos , Cadeias beta de HLA-DQ/genética , Antígenos HLA-B/genéticaRESUMO
Characterization of the novel HLA-C*07:1093 allele in a Russian female bone marrow donor.
Assuntos
Antígenos HLA-C , Nucleotídeos , Humanos , Feminino , Antígenos HLA-C/genética , Alelos , Teste de Histocompatibilidade , Éxons/genética , Doadores de TecidosRESUMO
HLA-B*13:181 differs from HLA-B*13:02:01:01 by one nucleotide substitution in codon 72 in exon 2.
Assuntos
Genes MHC Classe I , Antígenos HLA-B , Humanos , Alelos , Teste de Histocompatibilidade , Códon , Antígenos HLA-B/genética , Análise de Sequência de DNARESUMO
Three novel HLA-DRB1 alleles, HLA-DRB1*07:150, HLA-DRB1*13:345 and HLA-DRB1*14:256, were identified.
Assuntos
Cadeias HLA-DRB1 , Humanos , Cadeias HLA-DRB1/genética , Alelos , Sequência de BasesRESUMO
One nucleotide substitution in pseudoexon 5 of HLA-DQB1*06:02:01:01 results in a novel allele, HLA-DQB1*06:02:01:32.
Assuntos
Nucleotídeos , Humanos , Alelos , Cadeias beta de HLA-DQ/genética , Federação RussaRESUMO
HLA genes play a major role for successful hematopoietic stem cell transplantation (HSCT). While the success of HSCT depends on a HLA compatibility between donor and patient, finding a suitable donor remains challenging because of the high polymorphic nature of HLA genes. In this study, HLA-A, -B, -C, -DRB1 and -DQB1 alleles were genotyped at the 3-fields resolution level using MiSeq Illumina of 3341 Russian volunteers from the Kirov bone marrow Registry. Full gene of HLA-A, -B and -C, exons 2-4 of HLA-DRB1 and exons 1-5 of HLA-DQB1 were amplified by multiplex long-range polymerase chain reaction (PCR) and each allele was determined by matching the targeted regions and the reference sequence consisting of the IPD-IMGT/HLA Database. A total of 79 alleles of HLA-A, 115 alleles of HLA-B, 67 alleles of HLA-C, 71 alleles of HLA-DRB1 and 34 alleles of HLA-DQB1 were identified. According to common, intermediate and well-documented catalogs, 38 alleles in HLA-A, 69 in HLA-B, 39 in HLA-C, 48 in HLA-DRB1 and 21 in HLA-DQB1 locus were common alleles, and 5, 7, 7, 7, 2 kinds, accordingly, to written above were well-documented alleles. A total of 12 novel alleles including 3 alleles in HLA-A, 3 alleles in HLA-B, 1 allele in HLA-C, 2 alleles in HLA-DRB1 and 3 alleles in HLA-DQB1 loci were found. Six haplotypes with a frequency of more than 1.0% accounted for 13.19% of the total haplotype frequencies. This information on rare and novel alleles found by HLA typing with NGS may be helpful for unrelated HSCT among Russians.
Assuntos
Antígenos HLA-C , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Alelos , Antígenos HLA-C/genética , Cadeias HLA-DRB1/genética , Frequência do Gene , Antígenos HLA-A/genética , Antígenos HLA-B/genética , Haplótipos , Cadeias beta de HLA-DQ/genética , VoluntáriosRESUMO
Three new HLA alleles characterized by next-generation sequencing, HLA-A*68:298, HLA-C*07:1054N and HLA-DRB1*15:216.
Assuntos
Genes MHC Classe I , Antígenos HLA-C , Humanos , Cadeias HLA-DRB1/genética , Antígenos HLA-C/genética , Alelos , Sequenciamento de Nucleotídeos em Larga EscalaRESUMO
One nucleotide substitution in pseudoexon 5 of HLA-DQB1*05:02:01:01 results in a novel allele, HLA-DQB1*05:02:01:15.
Assuntos
Medula Óssea , Doadores de Tecidos , Humanos , Alelos , Cadeias beta de HLA-DQ/genéticaRESUMO
HLA-DQB1*02:02:23 differs from HLA-DQB1*02:02:01:01 by one synonymous nucleotide substitution at codon -6 in exon 1.
Assuntos
Células-Tronco Hematopoéticas , Humanos , Sequência de Bases , Alelos , Alinhamento de Sequência , Cadeias beta de HLA-DQ/genéticaRESUMO
Two novel HLA alleles, HLA-B*58:140 and HLA-DRB1*07:145, were identified.
Assuntos
Genes MHC Classe I , Antígenos HLA-B , Humanos , Alelos , Cadeias HLA-DRB1/genética , Antígenos HLA-B/genética , Federação RussaRESUMO
Two new HLA alleles were identified during routine next generation sequencing.
Assuntos
Antígenos HLA-A , Antígenos HLA-C , Humanos , AlelosRESUMO
HLA-C*15:250 differs from HLA-C*15:02:01:01 in codon 120 in exon 3.
Assuntos
Medula Óssea , Antígenos HLA-C , Alelos , Sequência de Bases , Antígenos HLA-C/genética , Teste de Histocompatibilidade , Humanos , Análise de Sequência de DNA , Doadores de Tecidos , VoluntáriosRESUMO
The novel HLA-DRB1*13:03:12 allele was characterized using two next-generation sequencing technologies.
Assuntos
Sequenciamento de Nucleotídeos em Larga Escala , Alelos , Cadeias HLA-DRB1/genética , HumanosRESUMO
Two new HLA alleles were identified during routine next generation sequencing.
Assuntos
Antígenos HLA-B , Sequenciamento de Nucleotídeos em Larga Escala , Alelos , Antígenos HLA-B/genética , Cadeias HLA-DRB1/genética , Teste de Histocompatibilidade , HumanosRESUMO
Identification of the novel HLA-A*68:99:02 allele that differs from HLA-A*68:99:01 in exon 2 and intron 7.
Assuntos
Antígenos HLA-A , Doadores não Relacionados , Alelos , Éxons/genética , Antígenos HLA-A/genética , Sequenciamento de Nucleotídeos em Larga Escala , HumanosRESUMO
Seven novel HLA alleles, HLA-A*01:407, -A*01:408, -A*03:434, -B*40:508N, -B*40:511N, -DRB1*04:336, and -DRB1*11:297Q in individuals from Irkutsk Region.
