RESUMO
A novel alkaloid, hupcrispatine (1), has been isolated from the unique Chinese species Huperzia crispate Ching. The structure of hupcrispatine has been elucidated as 9-amino-6-methyl-3-quinolone on the basis of spectral evidence.
Assuntos
Alcaloides/química , Huperzia/química , Quinolinas/química , Estrutura MolecularRESUMO
To study the constituents of Rubus corchorifolius L. f, the constituents were separated by using various chromatographies and the structures were identified on the basis of chemical and spectral analysis. Two compounds were isolated from Rubus corchorifolius L. f. Their structures were identified as ent-kauran-3beta, 16beta, 17, 19-tetrol (1), ent-2-carbonyl-16beta-hydroxy-kauran-17-beta-D-glucoside (2). Compounds 1 and 2 are new compounds.
Assuntos
Diterpenos do Tipo Caurano/isolamento & purificação , Glucosídeos/isolamento & purificação , Rosaceae/química , Diterpenos do Tipo Caurano/química , Glucosídeos/química , Estrutura Molecular , Folhas de Planta/química , Plantas Medicinais/químicaRESUMO
OBJECTIVE: To study the effects of Tengcha flavonoids (TCF) on scavenging oxygen free radicals and inhibiting lipid-peroxidation. METHODS: The effects of TCF on scavenging superoxide anion radical induced by xanthine-xanthine oxidase, inhibiting lipid-peroxidation of heart, liver, brain tissue homogenate and mitochondria induced by Fe2+ -VitC, Fe2+ -H2O2, Fe-Cys were discussed by TBA spectrometric method. RESULTS: TCF could significantly inhibit the superoxide anion radical, IC50 is 14.7 mg/L, and the lipid peroxidation of liver homogenate and mitochondria induced by auto-oxidation and induced-oxidation. The inhibition exhibited concentration-dependent manner. CONCLUSION: It indicated that TCF could scavenge oxygen free radicals and was capable of prophylacticly antagonizing against the oxidative injury by superoxide anion.
Assuntos
Ampelopsis/química , Flavonoides/farmacologia , Sequestradores de Radicais Livres/farmacologia , Plantas Medicinais/química , Animais , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Flavonoides/isolamento & purificação , Sequestradores de Radicais Livres/isolamento & purificação , Radicais Livres/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Dilatação Mitocondrial/efeitos dos fármacos , Oxigênio/metabolismo , Ratos , Ratos WistarRESUMO
OBJECTIVE: To find the active constituents of Rosa bracteata Wendl. to antagonize withdrawal syndrome in morphinedependent mice. METHODS: The constituents were isolated and purified by silica gel chromatography repeatedly. Their structures were made clear by chemical reactions and on the evidence of spectroscopy. RESULTS AND CONCLUSIONS: Five compounds were isolated from the fruits and four of them were determined as trimethly citrate, dimethyl 3-carboxyl-3-hydroxypentanedioate, 3-hydroxy-3-methoxycarbonyl-pentanedioic acid, calcium citrate, which were found from the Rose genus plant for the first time.
Assuntos
Citrato de Cálcio/isolamento & purificação , Citratos/isolamento & purificação , Plantas Medicinais/química , Rosa/química , Citrato de Cálcio/análise , Citratos/análise , Medicamentos de Ervas Chinesas/química , Frutas/química , Cromatografia Gasosa-Espectrometria de Massas , Peso Molecular , Espectrofotometria UltravioletaRESUMO
OBJECTIVE: To study the effects of Dihydromyricetin (DMY) on antilipid-peroxidation. METHOD: The antilipid-peroxidation of DMY on heart, liver, brain tissue homogenate and mitochondria was measured by the determination of malondiadehyde (MDA) induced by Fe2+ -Vit C, Fe2+ -H2O2, Fe-Cys with TBA spectrometric method. RESULT: DMY could inhibit the lipid peroxidation of homogenate and mitochondria. The inhibition exhibited concentration-dependent manner. CONCLUSION: DMY has good antilipid-peroxidation effect, which is worth studing further.
Assuntos
Antioxidantes/farmacologia , Flavonóis/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Ampelopsis/química , Animais , Antioxidantes/isolamento & purificação , Encéfalo/metabolismo , Relação Dose-Resposta a Droga , Flavonóis/isolamento & purificação , Fígado/metabolismo , Masculino , Malondialdeído/metabolismo , Mitocôndrias/metabolismo , Dilatação Mitocondrial/efeitos dos fármacos , Miocárdio/metabolismo , Plantas Medicinais/química , RatosRESUMO
OBJECTIVE: To study chemical constituents of Ilex kudingcha. METHOD: The constituents were isolated by chromatographic method and the structures were identified on the basis of spectral analysis. RESULT: Seven compounds, lupeol(I); 3 beta-hydroxyl-lup-20(29)-ene-24-methyl ester(II); lup-20(29)-ene-3 beta-, 24-diol(III); beta-sitosterol(IV); ursolic acid(V); daucosterol (VI); mannitol were obtained(VII). CONCLUSION: I, III, VI and VII compounds were obtained from genus Ilex for the first time.
