Effect of Low-Frequency Pulsed Electromagnetic Fields and Traditional Chinese Medicine Kneading Manipulation on Sternocostal Joint Pain in Perimenopausal Women.

Aim: To investigate the clinical effect of low-frequency pulsed electromagnetic fields (PEMFs) and Traditional Chinese Medicine (TCM) kneading manipulation in the treatment of perimenopausal women with sternocostal joint pain. Methods: A total of 80 perimenopausal women with osteoporosis (OP) with sternocostal joint pain were selected as participants in the study. The patients were assigned to either the control or the treatment group, with 40 patients in each group. Patients in the control group were treated with oral Aceclofenac sustained-release tablets, calcium carbonate and vitamin D3 tablets. The treatment group was treated with low-frequency pulsed electromagnetic fields and TCM kneading manipulation. Numerical rating scale (NRS) scores, bone mineral density (BMD) and blood calcium concentration were measured and recorded before and after treatment in both groups. Results: There were no significant differences in age, disease course, body mass index, smoking history, pretreatment NRS pain score, bone mineral density (BMD), or serum calcium concentration between the two groups (P > .05). There were statistically significant differences in pain levels between the two groups at 3 days and 1, 3 and 6 months after treatment (P < .05). BMD of the femoral neck was significantly different at 6 months after treatment (P = .016 treatment difference from Control at 6 months: 0.055; 95% CI, 0.009 to 0.097). There were significant differences in serum calcium concentration at the third and sixth month of treatment (P < .05 treatment difference from control at 3 days: 0.055; 95% CI: 0.036 to 0.074; treatment difference from Control at 6 months: 0.039; 95% CI: 0.019 to 0.059). Different treatment methods had significant differences in serum calcium levels at the third and sixth month. Conclusion: Low-frequency pulsed electromagnetic field and TCM kneading manipulation can effectively relieve the symptoms of thoracic and costal joint pain in the short term in the perimenopausal period, improve bone density and delay disease progression.

Differential Methylation and Transcriptome Integration Analysis Identified Differential Methylation Annotation Genes and Functional Research Related to Hair Follicle Development in Sheep.

Hair follicle growth and development are a complex and long-term physiological process, which is regulated by a variety of physical factors and signal pathways. Increasing the understanding of the epigenetic regulation and function of candidate genes related to hair follicle development will help to better understand the molecular regulatory mechanisms of hair follicle development. In this study, the methylated DNA immunoprecipitation sequencing (MeDIP-seq) was used to obtain the genome-wide methylation map of the hair follicular development of Super Merino sheep in six stages (fetal skin tissue at 65d, 85d, 105d, 135d, 7d, and 30d after birth). Combined with the results of previous RNA-sequencing, 65 genes were screened out that were both differential methylation and differential expression, including EDN1, LAMC2, NR1D1, RORB, MyOZ3, and WNT2 gene. Differential methylation genes were enriched in Wnt, TNF, TGF-beta, and other signaling pathways related to hair follicle development. The bisulfite sequencing PCR results and MeDIP-seq were basically consistent, indicating that the sequencing results were accurate. As a key gene in the Wnt signaling pathway, both differential methylation and expression gene identified by MeDIP-seq and RNA-seq, further exploration of the function of WNT2 gene revealed that the DNA methylation of exon 5 (CpG11 site) promoted the expression of WNT2 gene. The overexpression vector of lentivirus pLEX-MCS-WNT2 was constructed, and WNT2 gene effectively promoted the proliferation of sheep skin fibroblasts. The results showed that WNT2 gene could promote the growth and development of skin and hair follicles. The results of this study will provide a theoretical basis for further research on sheep hair follicle development and gene regulation mechanisms.

Comparison of three ultrasonographic examinations on the synovial membrane vascularity of RA patients.

Rheumatoid arthritis (RA) is a systemic autoimmune disease involving multiple joints and often involves the small joints, and the lesions are symmetric, invasive, and disabling. Synovial blood flow in patients with RA was compared using color Doppler flow imaging (CDFI), power Doppler ultrasound (PDUS), and superb microvascular imaging (SMI) to determine the application value of SMI in synovial vasospasm of knee joints. The blood flow signals of the suprapatellar recess in the knee joints of 41 RA patients (49 knees) were measured prior to undergoing total knee arthroplasty (TKA), recorded, and graded by CDFI, PDUS, and SMI. The results of the three ultrasound examination methods were compared and analyzed. The SMI grading was compared with the pathologic grade of the synovial membrane. Forty-one patients underwent 49 TKAs. The display rate of the synovial blood flow signal was 93.9% in the CDFI model, 97.9% in the PDUS model, and 100% in the SMI model. There were statistically significant differences in the results between the three ultrasound examination methods (HC = 11.84, P < 0.05). The consistency of the SMI and pathologic grades of synovial membranes was better than the other methods (kappa = 0.639, P < 0.05). Compared with CDFI and PDUS, the signal of synovia flow detected by SMI was significantly higher in RA patients. SMI classification had a better consistency with the pathologic grade, and SMI has application value in assessing the activity of RA.

Comparison of diagnostic values of ultrasound micro-flow imaging and contrast-enhanced ultrasound for neovascularization in carotid plaques.

The aim of the present study was to compare the diagnostic values of ultrasound micro-flow imaging (SMI) and contrast-enhanced ultrasound (CEUS) for neovascularization in carotid plaques, and to investigate their capacities for predicting the risks of cerebral stroke. A total of 39 patients (64 carotid plaques) with severe carotid artery stenosis undergoing carotid endarterectomy were selected between February 2015 and February 2016, and SMI and CEUS were used to detect neovascularization in plaques. According to the CEUS dynamic graph of plaques, the enhanced intensity visual scales and contrast parameters were obtained. Carotid atherosclerotic plaques were divided into 4 groups. The differences in the enhanced intensity visual scales, contrast parameters, and gray-scale median (GSM) values among the 4 groups were analyzed. Carotid plaque tissue samples from patients were stained for CD34, and the consistency of the methods for the diagnosis of neovascularization in plaques was analyzed. The differences in GSM values, enhanced intensities, and enhanced densities among the 4 groups of plaques were statistically significant (F=29.365, χ2=29.025, χ2=30.871, P<0.001); the differences in enhanced intensities of carotid atherosclerotic plaques with different echo types were statistically significant (χ2=17.951, P<0.001). The enhanced intensity of plaques was negatively correlated with the GSM value (r=-0.376, P<0.01), and the enhanced density of plaques was negatively correlated with the GSM value (r=-0.252, P<0.01). SMI and CEUS grading had good consistency (κ=0.860>0), there were statistically significant differences in new vessel densities with different SMI gradings (P<0.001), and the clinical symptoms and severity were positively correlated with SMI grading (rs=0.592>0). In conclusion, SMI and CEUS have good consistency for evaluating neovascularization in carotid plaques, and have good clinical value for evaluating neovascularization in carotid plaques.

Diagnostic Efficacy of Ultrasonographic Characteristics of Thyroid Carcinoma in Predicting Cervical Lymph Node Metastasis.

The goals of this study were to determine the ultrasonographic characteristics of thyroid carcinoma (TC) and to explore the diagnostic efficacy of these ultrasonographic characteristics in predicting cervical lymph node metastasis (LNM). From June 2012 to June 2014, a total of 186 TC patients were recruited from the Central Hospital of Chengde City, Hebei, China. We divided them into two groups: the metastatic group comprised 129 nodules (n = 86), and the non-metastatic group 117 nodules (n = 100). Univariate and multivariate analyses were used to evaluate the relationship between ultrasonographic characteristics and cervical LNM. Spectral Doppler ultrasound was employed to estimate peak systolic velocity, pulsatility index and resistive index. Receiver operating characteristic curves were drawn to evaluate the efficacy of ultrasonographic characteristics in predicting cervical LNM. The sensitivity, specificity, positive predictive value and negative predictive value of ultrasonographic diagnosis were 81.40% (105/129), 92.32% (108/117), 92.11% (105/114) and 81.82% (108/132), respectively. Cervical LNM in TC frequently occurred at the cervical level VI (37.98%) and was located mainly in the middle pole (46.51%) or lower pole (41.09%). Peak systolic velocity and resistive index values were significantly higher in the metastatic group than in the non-metastatic group (both p < 0.001). Multivariate analysis revealed that nodular diameter, capsular invasion, microcalcification and flow grade were risk factors for TC patients with cervical LNMs (all p < 0.05). Furthermore, receiver operating characteristic curve analysis revealed that nodular diameter, capsular invasion, microcalcification and flow grade had excellent accuracy in predicting cervical LNM. We conclude that ultrasonographic characteristics of TC, including maximum nodular diameter, capsular invasion, microcalcification and flow grade, may predict cervical LNM.