Associations between microbial presence in follicular fluid with IVF outcomes: a systematic review and meta-analysis.

PURPOSE: The aim of the study was to synthesize disparate studies to investigate potential impact of microbial presence in FF of infertile women on IVF outcomes. METHODS: Following preliminary searches to find medical subject heading (MeSH) terms plus free terms, a systematic search was performed in the PubMed, Cochrane Library, Embase, Web of Science, and Clinicaltrials.gov databases from January 10, 2022, to July 5, 2023. Data collected for each study were analyzed using RevMan 5.4 software available on the Cochrane website. RESULTS: After correcting for contamination from the vagina, the FFs of 289 women were detected positively by microbial culture and identification, ELISA, and IPA. The pregnancy rate of the FF-positive group was significantly lower than the FF-negative group (19.7% vs. 32.2%) and (OR: 0.57, 95% CI: 0.28-1.14, P=0.11; I2=56%) while the fertilization rate was almost equal (60.0% vs. 62.0%) and (OR: 1.03, 95% CI: 0.88-1.20, P=0.72; I2=0%). Evidence quality was very low. CONCLUSIONS: The different species of microorganisms in FF of infertile women may have different effects on IVF outcomes. The Lactobacillus spp. may have a positive effect, while other microorganisms may have the opposite effect.

A transmembrane protein family gene signature for overall survival prediction in osteosarcoma.

The transmembrane (TMEM) protein family is constituted by a large number of proteins that span the lipid bilayer. Dysregulation of TMEM protein genes widely occurs and is associated with clinical outcomes of patients with multiple tumors. Nonetheless, the significance of TMEM genes in the prognosis prediction of patients with osteosarcoma remains largely unclear. Here, we comprehensively analyzed TMEM protein family genes in osteosarcoma using public resources and bioinformatics methods. Prognosis-related TMEM protein family genes were identified by the univariate Cox regression analysis and were utilized to construct a signature based on six TMEM protein family genes (TMEM120B, TMEM147, TMEM9B, TMEM8A, TMEM59, and TMEM39B) in osteosarcoma. The prognostic signature stratified patients into high- and low-risk groups, and validation in the internal and external cohorts confirmed the risk stratification ability of the signature. Functional enrichment analyses of differentially expressed genes between high- and low-risk groups connected immunity with the prognostic signature. Moreover, we found that M2 and M0 macrophages were the most abundant infiltrated immune cell types in the immune microenvironment, and samples of the high-risk group showed a decreased proportion of M2 macrophages. Single-sample gene set enrichment analysis revealed that the scores of neutrophils and Treg were markedly lower in the high-risk group than these in the low-risk group in The Cancer Genome Atlas and GSE16091 cohorts. As for the related immune functions, APC co-inhibition and cytolytic activity exhibited fewer active levels in the high-risk group than that in the low-risk group in both cohorts. Of the six TMEM genes, the expression of TMEM9B was lower in the high-risk group than in the low-risk group and was positively associated with the overall survival of osteosarcoma patients. In conclusion, our TMEM protein family gene-based signature is a novel and clinically useful prognostic biomarker for osteosarcoma patients, and TMEM9B might be a potential therapeutic target in osteosarcoma.

Application of Doctor-Nurse-Patient Co-Decision-Making Nursing Intervention Based on Evidence-Based Problems in the Rehabilitation of Acute Ankle Lateral Collateral Ligament Injury.

Objective: The aim of this study is to study the application effects of doctor-nurse-patient co-decision-making nursing intervention based on evidence-based problems in the rehabilitation of acute ankle lateral collateral ligament injury. Methods: 150 patients with acute ankle lateral collateral ligament injury who were treated in the hospital between December 2020 and December 2021 were selected, and they were divided into the routine group and the evidence-based group by the random number table method, with 75 cases in each group. The patients in the routine group received routine nursing intervention, while the patients in the evidence-based group adopted doctor-nurse-patient co-decision-making nursing intervention based on evidence-based problems, and both groups were intervened for 1 month. The rehabilitation time (swelling subsidence time, fixation removal time, and normal walking time), ankle active range of motion (dorsiflexion and plantar flexion), ankle function (ankle Kofoed score) before and after intervention, and the total incidence rate of complications (tendon injury, ankylosis, and traumatic arthritis) within 1 month of intervention were compared between the two groups of patients. Results: The swelling subsidence time, fixed removal time, and normal walking time in the evidence-based group were significantly shorter than those in the routine group (P < 0.05). After 1 month of intervention, the ranges of motion of dorsiflexion and plantar flexion and ankle Kofoed scores of the two groups were significantly higher than those before intervention, and the abovementioned indicators in the evidence-based group were significantly higher than those in the routine group (P < 0.05). Within 1 month of intervention, the total incidence rate of tendon injury, ankylosis, and traumatic arthritis was significantly lower in the evidence-based group than that in the routine group (P < 0.05). Conclusion: Nursing intervention of doctor-nurse-patient co-decision-making based on evidence-based problems in patients with acute ankle lateral collateral ligament injury can promote postoperative rehabilitation and restore the ankle function of patients.