Bioinformatic analysis of CHEK1 as a marker of glomerular epithelial cell injury in diabetic nephropathy.

Diabetic nephropathy (DN) is considered to be a kidney disease caused by diabetes. In recent years, the incidence of DN has been on the rise, which is also a major challenge in the treatment and prognosis of the disease. Therefore, the search for new biomarkers of DN is urgent and has important clinical significance for reducing the morbidity and mortality of DN. In this study, two datasets GSE1009 and GSE142153 were selected to extract expression profile-based data from DN glomerular samples, and 238 differentially expressed genes (DEGs) were screened. Then, through enrichment analysis, the biological function of DEGs involved in DN disease was preliminarily explored. Subsequently, the STRING website was used to construct a protein-protein interaction map (PPI) to find 10 key genes (CHEK1, ITGB3, COL4A2, COL4A5, COL4A3, COL4A4, CCNB2, CCNB1, TPX2, KIF11), Which play an important role in the progression of DN disease and are closely related to other genes. CHEK1 was the focus of this study, and the expression level of CHEK1 in glomerular epithelial cell models was verified by qRT-PCR. Our results suggest that CHEK1 is a potential biomarker of the degree of damage to DN glomerular epithelial cells.

Effect of Hydraulic Expansion on Arteriovenous Fistula of Hemodialysis Patients.

BACKGROUND: Chronic kidney disease has become a serious public health problem in China. Our study is to explore effect of hydraulic expansion on arteriovenous fistula (AVF) of hemodialysis patients. METHODS: A total of 190 patients with end-stage renal disease (ESRD) were randomly divided into hydraulic expansion group (n = 117) and conventional surgery group (n = 73). Age, sex, the cause of ESRD, height, weight, body mass index (BMI), blood pressure, and diameter of artery and vein from ultrasonography before surgery from patients were recorded. Doppler ultrasonography of vessel was performed with a 12-MHz scanning probe for vascular measurements. The time of first cannulation was recorded. Primary and secondary patency rates were compared between the two groups. RESULTS: The mean arterial pressure for this cohort of patients was around 98.12 mm Hg. The mean diameters of artery and vein ready for anastomoses measured by ultrasonography before surgery were 1.96 and 2.04 mm, respectively. Age, weight, BMI, sex ratio, the cause of renal failure, history of catheter insertion, mean arterial pressure, frequency of hemodialysis, blood flow of hemodialysis, and the mode of anastomoses of AVF in conventional surgery group were similar to hydraulic expansion group. There were no differences in stroke volume of radius arterial and venous pressure before dilation between the two groups. The stroke volume of radius artery increased significantly after hydraulic expansion than before dilation and control group. The primary patency rates of AVF in patients with hydraulic expansion were higher significantly than conventional surgery group. The secondary patency rates in conventional surgery group were not different from hydraulic expansion group. CONCLUSION: Hydraulic expansion showed no difference from conventional surgery in complication after operation, and could decrease the time reliance on catheters and the risk of catheter-related infection, thrombosis, and decrease the related medical care costs.

Hydraulic expansion facilitates remodeling of arteriovenous fistulas without increasing venous intimal hyperplasia in rabbits.

Background: An arteriovenous fistula (AVF) is considered essential for chronic hemodialysis. Objective: To determine the effects of hydraulic expansion on the intimal hyperplasia of an AVF. Methods: We divided 12 healthy male New Zealand white rabbits into a control group (vein without special handling and direct anastomosis with an artery, n = 6) and a hydraulic expansion group (vein dilated by hydraulic pressure before anastomosis, n = 6). Histopathomorphology was examined with hematoxylin and eosin staining and immunohistochemistry. Analysis of covariance (ANCOVA) was used to compare the data between the groups. Results: Immediately and 1 day after surgery, the diameter of the fistula vein in rabbits in the hydraulic expansion group was significantly larger than it was in the control group (P = 0.02 and 0.03 respectively), but not on subsequent days. After hydraulic expansion and before construction of the fistula, the wall of vein was noticeably thinner on macroscopic observation, and the anterior and posterior walls were indistinguishable. At 3 weeks after surgery in the hydraulic expansion group, cells in the vein wall were disordered, there were fewer elastic fibers, tissues from the endothelium to tunica externa were less dense, and there was less extracellular matrix than in the control group. Expression of connective tissue growth factor in the hydraulic expansion group was significantly less than that in the control group (P = 0.01). No differences were found in intimal thickness or immunohistochemistry scores for transforming growth factor-ß1 between the groups. Conclusion: Hydraulic expansion did not increase intimal hyperplasia of an AVF, but facilitates remodeling of AVFs in rabbits.

Maleic Anhydride-Grafted Isotactic Polybutene-1 and Modified Polyamide 6.

Maleic anhydride (MAH)⁻divinyl benzene (DVB) multi-monomer melt-grafting onto isotactic polybutene-1 (iPB-1) was carried out in a torque rheometer. The effects of dicumyl peroxide (DCP), MAH, and DVB concentrations, and temperature, on the reaction, were investigated. The optimized conditions were 170 °C, DVB/MAH = 4:6 (mass ratio). DVB as a comonomer enhanced the grafting degree (Gd) and grafting efficiency (Ge) of iPB-g-MAH better than styrene. The initiator DCP had little effect on Gd as its concentration over 0.2 phr, but the grafts' melt flow rate (MFR) increased significantly, and relative molecular weight decreased remarkably with increased DCP concentration. With increasing Gd, the contact angle of grafts with water decreased, and there was a larger crystallization rate. The study of iPB-1 and iPB-g-MAH (Gd = 1.5%)-modified polyamide 6 (PA6) showed that iPB-g-MAH had an obviously toughening effect on PA6. With increasing iPB-g-MAH concentration, the blends of impact strength and elongation at break increased obviously, tensile strength decreased slightly, and MFR decreased prominently, which greatly slowed the processing degradation of PA6. The properties of iPB-1/PA6 blends deteriorated. Both DSC curves and SEM micrographs confirmed that PA6/iPB-g-MAH blends had much better compatibility than PA6/iPB. The reason was that the anhydride group in iPB-g-MAH reacted with amide group in PA6 to improve the compatibility between two phases, and iPB-g-MAH is an excellent modifier for PA6.

Early use of autogenous arteriovenous fistula in patients with urgent hemodialysis.

PURPOSE: This study was designed to evaluate the long-term patency rate and complications associated with early use of the autogenous forearm arteriovenous fistula (AFAVF) in patients needing urgent hemodialysis. METHODS: The clinical data of all patients undergoing AFAVFs for hemodialysis access between June 1996 and June 2016 were retrospectively evaluated. The primary and secondary patencies were estimated using the life table method. RESULTS: A total of 104 AFAVFs were created for 102 patients. All patients had entered hemodialysis when fistulas were constructed. The mean time to the first cannulation of the AFAVF was 17.33 ± 4.60 (5-27 days). Four AFAVFs (3.8%) became occluded within 30 days of creation of the access, and five AFAVFs (4.8%) had hematomas after cannulation. There were no cases of infection of the wound or steal syndrome or prolonged arm edema. The primary patency rate was 77.81% at 1 year, 73.05% at 2 years, 64.64% at 3 years, 60.75% at 5 years and 47.48% at 10 years. The secondary patency rate was 96.78% at 1 year, 95.18% at 5 years and 85.81% at 10 years. CONCLUSIONS: In this study, the patency rates following the early use of the AFAVFs were not inferior to the previously reported patency rates in the literature. For patients entering hemodialysis with an inserted central catheter, the early use of the AFAVFs decreases the complications associated with catheters.