Species Richness of Papilionidae Butterflies (Lepidoptera: Papilionoidea) in the Hengduan Mountains and Its Future Shifts under Climate Change.

The family of Papilionidae (Lepidoptera: Papilionoidea) is a group of butterflies with high ecological and conservation value. The Hengduan Mountains (HMDs) in Southwest China is an important diversity centre for these butterflies. However, the spatial distribution pattern and the climate vulnerability of Papilionidae butterflies in the HDMs remain unknown to date. The lack of such knowledge has already become an obstacle in formulating effective butterfly conservation strategies. The present research compiled a 59-species dataset with 1938 occurrence points. The Maxent model was applied to analyse the spatial pattern of species richness in subfamilies Parnassiinae and Papilioninae, as well as to predict the response under the influence of climate change. The spatial pattern of both subfamilies in the HDMs has obvious elevation prevalence, with Parnassiinae concentrated in the subalpine to alpine areas (2500-5500 m) in western Sichuan, northwestern Yunnan and eastern Tibet, while Papilioninae is concentrated in the low- to medium-elevation areas (1500-3500 m) in the river valleys of western Yunnan and western Sichuan. Under the influence of climate change, both subfamilies would exhibit northward and upward range shifts. The majority of Parnassiinae species would experience drastic habitat contraction, resulting in lower species richness across the HDMs. In contrast, most Papilioninae species would experience habitat expansion, and the species richness would also increase significantly. The findings of this research should provide new insights and a clue for butterfly diversity and climatic vulnerability in southwestern China. Future conservation efforts should be focused on species with habitat contraction, narrow-ranged distribution and endemicity with both in situ and ex situ measures, especially in protected areas. Commercialised collecting targeting these species must also be regulated by future legislation.

Checklist of Yunnan Papilionidae (Lepidoptera: Papilionoidea) with nomenclatural notes and descriptions of new subspecies.

A checklist of the Papilionidae of Yunnan is presented, with nomenclatural and taxonomic changes made. In the nomenclatural section, the junior homonym Papilio bootes nigricans Rothschild, 1895 is replaced by Papilio bootes nigricauda Lamas & Cotton nom. nov., Chilasa (Cadugoides) epycides muhabbet Koak, 2005 is synonymised with Papilio epycides camilla Rousseau-Decelle, 1947 syn. nov., Graphium cloanthus nyghmat Koak & Kemal, 2000 is placed as a junior objective synonym syn. nov. of Graphium cloanthus clymenus (Leech, 1893), and Papilio astorion Westwood, 1842 is shown to have priority over Papilio varuna White, 1842, thus the valid species name is Atrophaneura astorion (Westwood, 1842) comb. nov. In the main checklist, five new subspecies are described: Parnassius cephalus haba Hu & Cotton ssp. nov., Lamproptera curius hsinningae Hu, Zhang & Cotton ssp. nov., Lamproptera curius yangtzeanus Hu & Cotton ssp. nov., Graphium macareus vadimi Cotton & Hu ssp. nov., and Papilio krishna benyongi Hu & Cotton ssp. nov. The First Reviser Principle under the ICZN Code is invoked to solve four taxonomic problems, and 18 names are synonymised with explanations, notably Papilio machaon venchuanus Moonen, 1984 syn. nov., which is synonymised with Papilio machaon schantungensis Eller, 1936. Byasa genestieri (Oberthr, 1918) stat. nov. is separated from Byasa latreillei (Donovan, 1826), and Papilio everesti Riley, 1927 stat. nov. and P. verityi Fruhstorfer, 1907 stat. nov. are separated from Papilio machaon Linnaeus, 1758 as species. Taxa that require further confirmation of their presence in Yunnan and those that do not occur in Yunnan are enumerated.

Complete mitochondrial genomes of two insular races of Pazala swordtails from Taiwan, China (Lepidoptera: Papilionidae: Graphium).

The mitogenomes of two insular subspecies of Pazala, G. (P.) eurous asakurae and G. (P.) mullah chungianus from Taiwan Island, are reported. Both mitogenomes are circular, 15,228 bp and 15,240 bp in length respectively, and consist of 37 genes, including 13 PCGs, 22 tRNAs, and two rRNAs. The Bayesian phylogenetic tree containing the focal taxa and 31 other Papilioninae members clustered them with G. (P.) mullah (Alphéraky, 1897) and then G. (P.) parus (Nicéville, 1900) inside tribe Leptocircini, which agrees with their taxonomic positions. The findings of this study would benefit future understanding of phylogeography and conservation of subgenus Pazala.

Revision of Pazala Moore, 1888: The Graphium (Pazala) alebion and G. (P.) tamerlanus Groups, with Notes on Taxonomic and Distribution Confusions (Lepidoptera: Papilionidae).

Three Graphium species belonging to two species groups of the subgenus Pazala, the alebion and tamerlanus groups, were examined in molecular and morphological studies, and their female genitalia are reported for the first time. Their relationship with other species groups within the subgenus is assessed and their divergence times are estimated. We find that G. (P.) alebion is the first lineage to diverge within Pazala in the early Miocene (20 Ma) and that G. (P.) tamerlanus and G. (P.) parus are sister species and diverged from each other in the late Miocene (7 Ma). A revision of the four recognised taxa belonging to three species is presented, and historical misidentification of these taxa and their geographic ranges are explained.

Spatial Distribution of Pollinating Butterflies in Yunnan Province, Southwest China with Resource Conservation Implications.

Pollinating butterflies are an important asset to agriculture, which still depends on wild resources. Yunnan Province in Southwest China is a region with typical montane agriculture, but this resource is poorly investigated. From literature reference and specimen examination, the present study identified 554 species of pollinating butterflies (50.8% of the total butterflies) from Yunnan, with family Nymphalidae possessing the least number of pollinators (80 species, 16.0%), while the remaining four families are pollinator-rich (>73%). Tropical lowlands and mountain-valley areas possess higher species richness than those with plain terrains. The species richness of pollinating butterflies in Yunnan does not simply decline with the increase of latitude, nor is significantly different between West and East Yunnan. Zonation of pollinating butterflies using the parsimony analysis of endemicity (PAE) identified nine distribution zones and ten subzones. Most areas of endemism (AOE) are found in lowlands or mountain-valley areas, complexity of terrains, climates, and vegetation types are believed to be the main causes of such endemicity. The potential pollinating service of these butterflies could be great to montane agriculture with expanding areas of cash crops and fruit horticulture. Conservation strategies for pollinating butterflies may consist of preserving habitats and establishing butterfly-friendly agriculture based on local traditions.

Revision of Pazala Moore, 1888: The Graphium (Pazala) mandarinus (Oberthür, 1879) Group, with Treatments of Known Taxa and Descriptions of New Species and New Subspecies (Lepidoptera: Papilionidae).

The previously recognised closely related species Graphium (Pazala) mandarinus (Oberthür, 1879) and G. (P.) sichuanica (Koiwaya, 1993) are shown to comprise seven species as a result of both molecular and morphological analysis. Molecular dating analysis is also performed on the mandarinus group in order to investigate the divergence time of the taxa. Two taxa, G. (P.) garhwalica (Katayama, 1988) stat. nov. and G. (P.) paphus (de Nicéville, 1886) stat. nov., are raised from subspecific to specific status; G. (P.) hoeneanus Cotton Hu nom. nov., stat. rev. is separated from sichuanica at species level; and two previously unrecognised new species, G. (P.) daiyuanae Hu, Zhang Cotton sp. nov. and G. (P.) confucius Hu, Duan Cotton sp. nov. are described from Vietnam and China respectively, the latter being sympatric with nominate G. (P.) mandarinus. The identity of the lectotype of G. (P.) mandarinus is confirmed and a lectotype is designated for the taxon Papilio Glycerion Gray, 1831. A new subspecies of G. (P.) mandarinus is described from western Yunnan and northern Myanmar, G. (P.) mandarinus stilwelli Cotton Hu ssp. nov.