Effects of three physical exercise modalities on respiratory function of older adults with Parkinson's disease: A randomized clinical trial.

INTRODUCTION: Deficits in respiratory function of patients with Parkinson's disease contribute to aspiration pneumonia, one of the main causes of mortality in this population. The aim of this study was to evaluate the effects of functional training, bicycle exercise, and exergaming on respiratory function of elderly with Parkinson's disease. METHODS: A randomized clinical trial with single blinding was conducted in a public reference outpatient clinic for the elderly. The participants were randomly assigned to three groups. Group 1 was submitted to functional training (n = 18); group 2 performed bicycle exercise (n = 20), and group 3 trained with Kinect Adventures exergames (n = 20). The sessions performed lasted 8 weeks with a frequency of three 50-min sessions per week. The primary outcome was the forced expiratory volume in the first second; and the secondary outcomes were forced vital capacity, peak expiratory flow, and maximum inspiratory and expiratory pressures. RESULTS: The interventions performed did not improve the forced expiratory volume in the first second, forced vital capacity, and peak expiratory flow. However, group 2 improved (p = 0.03) maximum expiratory pressure (from 65.5cmH2O to 73.1cmH2O) (effect size 0.47), and group 3 increased (p = 0.03) maximum inspiratory pressure (from -61.3cmH2O to -71.6cmH2O) (effect size 0.53). CONCLUSIONS: No effect was found on lung volume, forced respiratory flow and capacity of the participants with Parkinson's disease submitted to three different modalities of motor training. However, bicycle exercise and exergaming have improved expiratory and inspiratory muscle strength, respectively. NCT02622737.

Functionalization with Polyphenols of a Nano-Textured Ti Surface through a High-Amino Acid Medium: A Chemical-Physical and Biological Characterization.

The study aimed to identify an effective mechanism of adsorption of polyphenols on a nano-textured Ti surface and to evaluate the osteogenic differentiation on it. The source of polyphenols was a natural extract from red grape pomace. A chemical etching was used to form an oxide layer with a nanoscale texture on Ti; this layer is hydrophilic, but without hydroxyl groups with high acidic-basic chemical reactivity. The samples were characterized by electron and fluorescence microscopies, UV-Vis spectroscopy, contact angle measurements, zeta potential titration curves, and Folin-Ciocâlteu test. The presence of an adsorbed layer of polyphenols on the functionalized surface, maintaining redox ability, was confirmed by several tests. Consistent with the surface features, the adsorption was maximized by dissolving the extract in a high-amino acid medium, with respect to an inorganic solution, exploiting the high affinity of amino acids for polyphenols and for porous titanium surfaces. The osteogenic differentiation was assessed on an osteoblastic cell line by immunofluorescence, cell viability, expression of key osteoblast markers, and extracellular matrix mineralization. The surfaces functionalized with the extract diluted in the range 1 × 10-5-1 mg/mL resulted in having a greater osteogenic activity for the highest concentration, with lower values of cell viability; higher expression of alkaline phosphatase, bone sialoprotein, and collagen; and lower levels of osteopontin. In conclusion, the functionalization of a nano-textured Ti surface with polyphenols can potentially favor the osteogenic activity of osseointegrated implants.

CHRONIC PANCREATIC DISEASE IN THE LESSER KUDU (TRAGELAPHUS IMBERBIS): A REPORT OF 16 CASES IN THE UNITED STATES.

A review of archival cases at Northwest ZooPath from 1995-2018 identified 16/96 (17%; eight females, eight males) lesser kudus (Tragelaphus imberbis) with chronic pancreatic disease (CPD) from three institutions, all of which originated from an initial founder stock of 12 animals. Ages at time of death or euthanasia ranged from 2 to 132 mo (average age = 69 mo). All cases had varying degrees of pancreatic acinar cell depletion, ductular hyperplasia, and fibrosis. Serum fructosamine, glucose, insulin, and insulin to glucose ratio collected close to time of death from 13 affected animals were not significantly different from controls (n = 19). Of these analytes, receiver operating characteristic analysis identified fructosamine as the best-performing analyte with an area under the curve 0.671 (95% confidence interval = 0.480-0.828; sensitivity = 58.3%, specificity = 84.2%; cutoff point >352 µmol/L) in the diagnosis of CPD. With a 15% prevalence, there was a positive predictive value of 41% and a negative predictive value of 92%, indicating that the probability of false positives is high, but of false negatives is low. An etiologic agent was not identified by histology (n = 16), transmission electron microscopy (n = 1), or enterovirus PCR (n = 2). Serum zinc and hepatic heavy metal analyses were judged to be within normal limits. Chronic pancreatic disease is considered an important cause of morbidity and mortality in the lesser kudu; serum chemistry analysis warrants further investigation in its use for diagnosis. The etiopathogenesis is not understood, but the absence of obvious causes, the occurrence also in very young animals, and the inbred lineage of lesser kudus in the United States suggest a genetic basis for this disease.

Ruptured popliteal artery aneurysm in a patient with a clinical diagnosis of Marfan syndrome.

The popliteal artery is the main site of occurrence of peripheral aneurysms. Acute presentations constitute a potential threat to limb viability and to life, especially in the event of rupture. Rupture is a rare event, but one that demands an immediate intervention decision to achieve a satisfactory treatment outcome. The gold standard treatment is conventional surgery, effecting repair by interposition of a great saphenous vein graft. Studies conducted in recent decades have found associations between Marfan Syndrome and peripheral aneurysms. This report presents a case of a ruptured left popliteal artery aneurysm successfully treated in an 82-year-old patient clinically diagnosed with previously unknown Marfan syndrome.

Fechamento de comunicação buco-sinusal utilizando o corpo adiposo bucal: Relato de Caso Clínico / Closure of oroantral communications using buccal fat pad: technique report

Introdução: As comunicações buco-sinusais (CBS) são complicações cirúrgicas relativamente frequentes na prática odontológica, relacionadas à extração dos molares superiores. As CBS permitem a passagem de alimentos e outras substâncias para o interior do seio maxilar, propiciando, assim, quadros infecciosos conhecidos como Sinusites. O tratamento das CBS é desafiador. Objetivo: Relatar um caso clínico de fístula bucossinusal tratada pela técnica de fechamento com o corpo adiposo bucal. Relato de caso: paciente do gênero masculino, 59 anos, leucoderma, sem alterações de ordem sistêmica, compareceu ao ambulatório de Pós-graduação da Universidade do Sagrado Coração relatando como queixa principal o fato de "sair líquidos pelo nariz" durante alimentação e havia iniciado após realizar exodontia de raiz residual do dente 16. O mesmo relatou que após a exodontia foi realizada uma tentativa de fechamento da fístula buco-sinusal através do reposicionamento do retalho vestibular e não foi obtido sucesso. Após 45 dias de condicionamento da mucosa do seio maxilar através de irrigações com soro fisiológico e clorexidina a 0,12% foi proposto ao paciente e realizado o fechamento da fístula buco-sinusal através da utilização do corpo adiposo bucal. Conclusão: atualmente para se alcançar o alto índice de sucesso no fechamento de comunicações busco-sinusais utilizamos esta técnica descrita, por ser um procedimento simples e que porprociona resultados satisfatórios ao paciente.

Introduction: Bucosinusal communications are relatively frequent surgical complications in dental practice. Although several conditions are related to this type of communication, the exodontia of upper posterior teeth presents as the main etiological factor. They are diagnosed through fistulas, which communicate the buccal and sinus cavities, thus allowing the passage of food and other substances into the maxillary sinus, thus providing infectious pictures known as Sinusites. Objective: through the report of a clinical case of buccosalusal fistula, we will focus on the success of the closure of buccomasinal communication with Bichat Adipose Body. Technique report: male patient, 59 years old, leucoderma, with no systemic alterations, who attended the University of the Sacred Heart Postgraduation outpatient clinic reporting as a main complaint the fact of "getting fluids through the nose" during feeding and that this had started after performing a residual root exodon of element 16. It also reported that after the exodontia, an attempt was made to close the buccosinusal fistula through the repositioning of the vestibular flap and no success was achieved. After 45 days of conditioning the maxillary sinus mucosa through irrigations with saline solution and 0.12% chlorhexidine, the patient was proposed and the buccosinusal fistula was closed using the Bichat Adipose Body. Conclusion: Today, in order to achieve the high success rate in the closure of buscossinusal communications, we use the Bichat Adipose Body, because it is a simple procedure and provides satisfactory results for the patient.

Rotura de aneurisma de artéria poplítea em paciente clinicamente diagnosticado com síndrome de Marfan / Ruptured popliteal artery aneurysm in a patient with a clinical diagnosis of Marfan syndrome

Resumo A artéria poplítea é o principal local para a ocorrência de aneurismas periféricos. Suas formas de apresentação agudas são potencialmente ameaçadoras à viabilidade do membro e à vida, dentre as quais destacamos a sua rotura. Apesar de ser um evento raro, sua rotura demanda rápida proposta de intervenção para satisfatório desfecho terapêutico. O tratamento padrão-ouro é o cirúrgico convencional e se dá pela interposição de veia safena magna. Trabalhos feitos nas últimas décadas vêm encontrando associações entre a síndrome de Marfan e aneurismas periféricos. Este relato apresenta um caso de um aneurisma de artéria poplítea esquerda roto tratado com sucesso em um paciente de 82 anos diagnosticado clinicamente como portador de síndrome de Marfan previamente desconhecida.

Abstract The popliteal artery is the main site of occurrence of peripheral aneurysms. Acute presentations constitute a potential threat to limb viability and to life, especially in the event of rupture. Rupture is a rare event, but one that demands an immediate intervention decision to achieve a satisfactory treatment outcome. The gold standard treatment is conventional surgery, effecting repair by interposition of a great saphenous vein graft. Studies conducted in recent decades have found associations between Marfan Syndrome and peripheral aneurysms. This report presents a case of a ruptured left popliteal artery aneurysm successfully treated in an 82-year-old patient clinically diagnosed with previously unknown Marfan syndrome.

Análise da mobilidade toracoabdominal de idosos com doença de parkinson, submetidos ao treinamento funcional, bicicleta estacionária e exergame: um ensaio clínico randomizado / Analysis of the thoracoabdominal mobility of elderly parkinson's disease that have done stationary bicycle, functional training and exergaming: a randomized controlled trial

Introdução: idosos com doença de Parkinson (DP) apresentam mobilidade toracoabdominal reduzida, o que os predispõe a complicações respiratórias, como a pneumonia aspirativa. Objetivo: avaliar a mobilidade toracoabdominal em idosos com doença de Parkinson, antes e após realizarem treino funcional, bicicleta estacionária e exergame. Metodologia: 58 idosos foram randomizados em três grupos: um que realizou treino funcional (GF) n=18, outro que fez bicicleta estacionária (GB) n=20 e o terceiro que realizou o exergame (GE) n=20. A mobilidade foi mensurada pela cirtometria, por avaliador cego, em três níveis: axilar, xifoideano e umbilical, antes e após as intervenções. Resultados: não foram encontradas diferenças estatisticamente significativas no coeficiente de amplitude nos níveis axilar e xifoide e umbilical nos três grupos. No nível umbilical, entretanto, houve um discreto acréscimo, comparando antes e após as intervenções GF de 2,1(4,52) cm para 3,1(4,27) cm; GB de 1,1(3,16) cm para 1,7(4,68) cm e GE de 0,5 (3,29) cm para 1,0 (3,68) cm. Conclusão: os três programas de exercícios propostos não foram eficazes na melhora da mobilidade torácica no nível axilar e xifoide e parecem provocar uma pequena melhora na mobilidade abdominal, no contexto respiratório.

Introduction: elderly with Parkinson's disease (PD), have reduced thoracoabdominal mobility, which predisposes to respiratory complications such as aspiration pneumonia. Objective: to assess thoracoabdominal mobility in elderly with PD before and after performing functional training, stationary bike and exergaming. Methodology: fifty eight (58) elderly people were randomized into three groups: functional training (GF) n = 18, stationary bike (GB) n = 20 and exergaming (GE) n = 20. Mobility was measured by cirtometry, by blind assessor, in three levels: axillary, xiphoid and umbilical; before and after the interventions. Results: no statistically significant differences were found in the amplitude coefficient at axillary and xiphoid and umbilical levels in the three groups. At the umbilical level, however, there was a slight increase comparing before and after GF interventions from 2.1 (4.52) cm to 3.1 (4.27) cm; GB of 1.1 (3.16) cm to 1.7 (4.68) cm and GE of 0.5 (3.29) cm to 1.0 (3.68) cm. Conclusion: the three exercise programs proposed were not effective in improving axillary and xiphoid thoracic mobility and appear to cause a slight improvement in abdominal mobility in the respiratory context.

Predictors of adverse drug reactions associated with ribavirin in direct-acting antiviral therapies for chronic hepatitis C.

PURPOSE: To identify factors associated with the development of adverse drug reactions (ADR) in ribavirin therapeutic regimens. METHODS: A multicenter, prospective study was conducted in three public health hospitals in Rio de Janeiro between November 2015 and March 2018. Inclusion criteria were defined by patient follow-up at pharmaceutical consultation at the time of drug dispensing as those who used sofosbuvir in combination with simeprevir, daclatasvir, and/or ribavirin. All patients were invited to participate in the study during the first interview. Adverse drug reactions were reported according to the treatment regimen and frequency of occurrence. Statistical analysis was used to compare adverse reactions between treatments and their associated factors. RESULTS: A total of 405 patients were included in the study (mean age 59.6 ± 9.6 years); 61.0% were female, 88.1% were infected with genotype 1, and 65.4% were cirrhotic. The most prescribed treatment was the combination of sofosbuvir, daclatasvir, and ribavirin (55.3%). The majority of patients reported at least one ADR during treatment (83.2%), of which fatigue, anemia, and headache were the most common. Being female (OR = 1.86, [1.08-3.20]) and use of ribavirin (OR: 2.39; 95% CI [1.38-4.13]) were predictors for the development of ADR, which was also associated with development of anemia (OR: 10.28; 95% CI: [5.78-18.30]). Treatment efficacy was 98.1%. CONCLUSIONS: Direct-acting antiviral has been shown to be safe and effective. Therefore, use of ribavirin is questionable due to associated adverse reactions and similar efficacy to other treatments.

Not Just Par for the Course: 73 Quaternary Germanides RE4 M2 XGe4 ( RE = La-Nd, Sm, Gd-Tm, Lu; M = Mn-Ni; X = Ag, Cd) and the Search for Intermetallics with Low Thermal Conductivity.

A total of 73 new quaternary rare-earth germanides RE4 M2 XGe4 ( RE = rare-earth metal; M = Mn-Ni; X = Ag, Cd) were prepared through reactions of the elements. The solid solution Nd4Mn2Cd(Ge1- ySi y)4 was also prepared under the same conditions and found to be complete over the entire range. All of these compounds adopt the monoclinic Ho4Ni2InGe4-type structure (space group C2/ m, a = 14.2-16.7 Å, b = 4.0-4.6 Å, c = 6.8-7.5 Å, ß = 106-109°), as revealed by powder X-ray diffraction analysis and single-crystal X-ray diffraction analysis on selected members. The structure determination of Nd4(Mn0.78(1)Ag0.22(1))2Ag0.83(1)Ge4 disclosed disorder of Mn and Ag atoms within the tetrahedral site and Ag deficiencies within the square planar site. Within the solid solution Nd4Mn2Cd(Ge1- ySi y)4, the end-members and two intermediate members were structurally characterized; as the Si content increases, the Cd sites become less deficient and the individual [Mn2 Tt2] layers contract but become further apart from each other. Electronic band structure calculations confirm that the Ag-Ge or Cd-Ge bonds are the weakest in the structure and thus prone to distortion. Thermal property measurements confirm expectations from machine-learning predictions that these quaternary germanides should exhibit low thermal conductivity, which was found to be <10 W m-1 K-1 for Nd4Mn2AgGe4.

The Effects of Functional Training, Bicycle Exercise, and Exergaming on Walking Capacity of Elderly Patients With Parkinson Disease: A Pilot Randomized Controlled Single-blinded Trial.

OBJECTIVES: To compare the effects of functional training, bicycle exercise, and exergaming on walking capacity of elderly with Parkinson disease (PD). DESIGN: A pilot randomized, controlled, single-blinded trial. SETTING: A state reference health care center for elderly, a public reference outpatient clinic for the elderly. PARTICIPANTS: Elderly individuals (≥60 years of age; N=62) with idiopathic PD (stage 2 to 3 of modified Hoehn and Yahr staging scale) according to the London Brain Bank. INTERVENTION: The participants were randomly assigned to three groups. Group 1 (G1) participated in functional training (n=22); group 2 (G2) performed bicycle exercise (n=20), and group 3 (G3) trained with Kinect Adventures (Microsoft, Redmond, WA) exergames (n=20). MAIN OUTCOME MEASURES: The primary outcome measure was the 6-minute walk test (6MWT); secondary outcome measures were the 10-m walk test (10MWT), sitting-rising test (SRT), body mass index, Parkinson Disease Questionnaire-39, World Health Organization Disability Assessment Schedule 2.0 (WHODAS 2.0), and 15-item Geriatric Depression Scale. RESULTS: All groups showed significant improvements in 6MWT (G1 P=.008; G2 P=.001; G3 P=.005), SRT (G1 P<.001; G2 P=.001; G3 P=.003), and WHODAS 2.0 (G1 P=.018; G2 P=.019; G3 P=.041). Only G3 improved gait speed in 10MWT (P=.11). G1 (P=.014) and G3 (P=.004) improved quality of life. No difference was found between groups. CONCLUSIONS: Eight weeks of exergaming can improve the walking capacity of elderly patients with PD. Exergame training had similar outcomes compared with functional training and bicycle exercise. The three physical exercise modalities presented significant improvements on walking capacity, ability to stand up and sit, and functionality of the participants.

Using the spirometry to indicate respiratory exercises for elderly with Parkinson's disease / O uso da espirometria na indicação de exercícios respiratórios para idosos com Doença de Parkinson

Abstract Introduction: Respiratory dysfunction is the main cause of death in Parkinson's disease (PD) patients and bronchoaspiration pneumonia is the most common clinical respiratory complication. Objective: To assess respiratory function of elderly with PD in mild to moderate phase of the disease. Methods: A cross-sectional study was carried. Elderly in 2 to 3 PD Hoehn & Yahr stage have participated. A single researcher has evaluated maximal inspiratory pressure (MIP), maximal expiratory pressure (MEP), thoracoabdominal amplitude, forced vital capacity (FVC) and expiratory volume in the first second (FEV1). Results: Sixty elderly have participated and their all spirometry and manovacuometry parameters presented significant differences (p < 0.05) comparing with predicted values, except for FVC (p = 0.25). Only umbilical level did not reach normal values on cirtometry parameters. Patients classified as a restrictive disorder presented significant decrease in thoracic expandability. However the participants classified as an obstructive disorder showed significant decrease in expiratory muscle strength and peak expiratory flows. Conclusion: Elderly in mild or moderate phase of PD presented reduction in respiratory parameters. Spirometry showed to be an important tool to evaluate respiratory function and to indicate the modality of respiratory exercise. Our results suggest the indication of thoracic flexibility exercises for patient with PD classified as restrictive disorder and strength exercise of respiratory muscles for those classified as obstructive disorder.

Resumo Introdução: Disfunções respiratórias são as principais causas de morte em pacientes com Doença de Parkinson (DP) e a pneumonia por bronco-aspiração é a complicação respiratória mais comum. Objetivo: Avaliar a função respiratória de idosos com doença de Parkinson (DP) em fase leve a moderada da doença. Métodos: Foi realizado um estudo transversal. Participaram idosos nos estágios 2 a 3 da classificação de Hoehn & Yahr. Um único pesquisador avaliou a pressão inspiratória máxima (PIM), pressão expiratória máxima (PEM), amplitude toracoabdominal, capacidade vital forçada (CVF) e volume expiratório forçado no primeiro segundo (VEF1). Resultados: Sessenta idosos participaram e todos os seus parâmetros de espirometria e manovacuometria apresentaram diferenças significativas (p < 0,05) em comparação com os valores previstos, exceto a CVF (p = 0,25). Somente o nível umbilical não atingiu valores normais nos parâmetros da cirtometria. Pacientes classificados como distúrbios restritivos apresentaram diminuição significativa na capacidade de expansão torácica. No entanto, os participantes classificados como transtornos obstrutivos mostraram diminuição significativa na força muscular expiratória e dos fluxos expiratórios máximos. Conclusão: Os idosos em fase leve ou moderada da DP apresentaram redução nos parâmetros respiratórios. A espirometria mostrou ser uma ferramenta importante para avaliar a função respiratória e para indicar a modalidade do exercício respiratório. Nossos resultados sugerem a indicação de exercícios de flexibilidade torácica para pacientes com DP classificados como distúrbios restritivos e exercícios de força da musculatura respiratória para aqueles classificados como desordem obstrutiva.

The effects of functional training and stationary cycling on respiratory function of elderly with Parkinson disease: a pilot study / Os efeitos do treino funcional e da bicicleta estacionária na função respiratória de idosos com Doença de Parkinson: um estudo piloto

Abstract Introduction: Respiratory function changes in patients with Parkinson's disease (PD) contribute to aspiration pneumonia, the main cause of mortality in PD population. Objective: To compare the effects of functional training and cycle exercise in respiratory function of elderly with PD. Methods: Twenty participants were randomised into two groups. Experimental group (EG) (n = 10) performed cycle exercise and control group (CG) (n = 10) was trained with functional training. A blinded researcher evaluated maximal inspiratory pressure (MIP), maximal expiratory pressure (MEP), thoracoabdominal amplitude, forced vital capacity (FVC) and forced expiratory volume in the first second (FEV1) before and after interventions. Results: Both groups did not improve significantly respiratory function. Only CG improved significantly FVC (p = 0.01). No differences were found between groups. Conclusion: 8-week exercise programs are not able to improve the respiratory function of elderly in 2 to 3 PD Hoehn & Yahr stage. Both functional training and cycle exercise do not to improve respiratory function in elderly with PD.

Resumo Introdução: Mudanças na função respiratória em pacientes com Doença de Parkinson (DP) contribuem para a pneumonia aspirativa, principal causa de mortalidade nessa população. Objetivo: Comparar os efeitos do treino funcional e do exercício em bicicleta estacionária na função respiratória de idosos com Doença de Parkinson (DP). Métodos: 20 participantes foram randomizados em 2 grupos. O grupo experimental (GE) (n = 10) realizou exercício em bicicleta estacionária e o grupo controle (GC) (n = 10) realizou o treino funcional. Um pesquisador cegado avaliou a pressão inspiratória máxima (PIM), a pressão expiratória máxima (PEM), a amplitude toracoabdominal, a capacidade vital forçada (CVF) e o volume expiratório forçado no primeiro segundo (VEF1) antes e após as intervenções. Resultados: Ambos os grupos não melhoraram significativamente a função respiratória. Apenas o GC melhorou de forma significante a CVF (p = 0.01). Nenhuma diferença foi encontrada entre os grupos. Conclusão: Programas de exercício com duração de 8 semanas não são capazes de melhorar a função respiratória de idosos com DP nos estágios 2 a 3 de Hoehn & Yahr. Ambos grupos que realizaram o treino funcional e exercício em bicicleta estacionária não melhora a função respiratória de idosos com DP.

Effect of finishing and polishing methods on surface roughness of autopolymerized acrylic resins / Efeito de métodos de acabamento e polimento sobre a rugosidade superficial de resinas acrílicas autopolimerizáveis

ABSTRACT Objective : To evaluate the surface roughness of two makes of autopolymerized acrylic resin (Classic Dencor® and Duralay®) with two different methods of finishing and polishing (conventional and using the Dhpro® finishing & polishing kit). Methods : A total of 20 specimens (10 of each make) was obtained using Zetalabor®--Zhermak condensation silicone molds. After polymerization, 20 blocks of resin were divided in two and subjected to two types of finishing and polishing, one at each end. The types of finishing and polishing were as follows: conventional (lathe) and using the Dhpro® finishing & polishing kit. Once finished and polished, the specimens were subjected to surface roughness testing using a roughness meter and were analyzed via the Student's t-test. Results : There is a statistically significant difference between the forms of polishing, unrelated to the brand of acrylic resin. Conventional polishing achieved a level of 0.12 µm for both resins and the DhPro® polishing presented roughness a little above 0.2 µm. Conclusion : Conventional polishing is superior to the DhPro® kit, as it achieves lower levels of roughness. There is no significant difference between the acrylic resins in terms of surface roughness after the finishing and polishing processes.

RESUMO Objetivo: Avaliar a rugosidade superficial de duas marcas de resina acrílica autopolimerizável (Clássico Dencor® e Duralay®) com dois diferentes métodos de acabamento e polimento (convencional e através do kit de acabamento e polimento da Dhpro®). Métodos: Um total de 20 corpos de prova (10 de cada marca) foi obtido através de moldes em Silicona de Condensação Zetalabor® - Zhermak. Após a polimerização, os 20 blocos de resina foram divididos ao meio e submetidos a dois tipos de acabamento e polimento, um em cada extremo. Os tipos de acabamento e polimento foram: convencional (em torno mecânico) e através do kit de acabamento e polimento da Dhpro®. Após acabados e polidos, os corpos de prova foram submetidos ao ensaio de rugosidade superficial através de um Rugosímetro e analisados por meio do teste "t" de Student. Resultados: Existe diferença significativamente estatística entre os polimentos, não relacionada à marca da resina acrílica. O polimento convencional atingiu um nivel de 0,12 µm em ambas as resinas e o polimento da DhPro® apresentou rugosidade um pouco acima de 0,2 µm. Conclusão: O polimento convencional é superior ao kit DhPro®, por atingir níveis de rugosidade mais baixos. Não existe diferença significativa entre as resinas acrílicas quanto à rugosidade superficial após os processos de acabamento e polimento.

Redox non-innocent bis(2,6-diimine-pyridine) ligand-iron complexes as anolytes for flow battery applications.

Diiminepyridines are a well-known class of "non-innocent" ligands that confer additional redox activity to coordination complexes beyond metal-centred oxidation/reduction. Here, we demonstrate that metal coordination complexes (MCCs) of diiminepyridine (DIP) ligands with iron are suitable anolytes for redox-flow battery applications, with enhanced capacitance and stability compared with bipyridine analogs, and access to storage of up to 1.6 electron equivalents. Substitution of the ligand is shown to be a key factor in the cycling stability and performance of MCCs based on DIP ligands, opening the door to further optimization.

HI-bone: a scoring system for identifying phenylisothiocyanate-derivatized peptides based on precursor mass and high intensity fragment ions.

Peptide sequence matching algorithms used for peptide identification by tandem mass spectrometry (MS/MS) enumerate theoretical peptides from the database, predict their fragment ions, and match them to the experimental MS/MS spectra. Here, we present an approach for scoring MS/MS identifications based on the high mass accuracy matching of precursor ions, the identification of a high intensity b1 fragment ion, and partial sequence tags from phenylthiocarbamoyl-derivatized peptides. This derivatization process boosts the b1 fragment ion signal, which turns it into a powerful feature for peptide identification. We demonstrate the effectiveness of our scoring system by implementing it on a computational tool called "HI-bone" and by identifying mass spectra of an Escherichia coli sample acquired on an Orbitrap Velos instrument using Higher-energy C-trap dissociation. Following this strategy, we identified 1614 peptide spectrum matches with a peptide false discovery rate (FDR) below 1%. These results were significantly higher than those from Mascot and SEQUEST using a similar FDR.