Hemicelluloses for fuel ethanol: A review.

Hemicelluloses currently represent the largest polysaccharide fraction wasted in most cellulosic ethanol pilot and demonstration plants around the world. The reasons are based on the hemicelluloses heterogeneous polymeric nature and their low fermentability by the most common industrial microbial strains. This paper will review, in a "from field to fuel" approach the various hemicelluloses structures present in lignocellulose, the range of pre-treatment and hydrolysis options including the enzymatic ones, and the role of different microbial strains on process integration aiming to reach a meaningful consolidated bioprocessing. The recent trends, technical barriers and perspectives of future development are highlighted.

Limits to sustained energy intake XII: is the poor relation between resting metabolic rate and reproductive performance because resting metabolism is not a repeatable trait?

Many studies have investigated the consequences of individual variation in resting metabolic rate at thermoneutrality (RMRt) on reproductive performance. Despite strong theoretical reasons for expecting such an association, results have generally been disappointing. A fundamental assumption of these studies is that RMRt is a repeatable trait. We examined repeatability of RMRt in female MF1 mice over short (15 days apart; N=238) and long intervals (110 days apart; N=33). In the long-term experiment, after the first RMRt measurement, females were separated in two groups: the first was kept virgin (N=16); the second was allowed to breed (N=17) and measured 15 days after they had weaned their pups. We also examined the association between RMRt and reproduction. We used Pearson's correlation (r) and intraclass correlation coefficients (rho) to estimate repeatability. There was a strong effect of body mass on RMRt for all measurements. Over the short interval, repeatability was significant for body mass (r=0.86; rho=0.86), RMRt (r=0.68; rho=0.68,) and residual-RMRt (r=0.58; rho=0.58). Over long intervals, repeatability of residual-RMRt was high in virgin females (r=0.59; rho=0.60), but not in the breeders (r=0.38; rho=0.39); body mass was repeatable only for non-breeders measured by r (r=0.55). There was no significant correlation between RMRt or residual-RMRt and litter size or litter mass. In conclusion, RMRt and residual-RMRt are highly repeatable traits in virgin MF1 female mice. The lack of association between non-reproductive RMRt and reproductive performance in MF1 mice does not come about because of its poor repeatability.

Supplementation requirements of brewery's spent grain hydrolysate for biomass and xylitol production by Debaryomyces hansenii CCMI 941.

The effect of nutrient supplementation of brewery's spent grain (BSG) hydrolysates was evaluated with respect to biomass and xylitol production by Debaryomyces hansenii. For optimal biomass production, supplementation of full-strength BSG hydrolysates required only phosphate (0.5 g l(-1) KH(2)PO(4)), leading to a biomass yield and productivity of 0.60 g g(-1) monosaccharides and 0.55 g l(-1 )h(-1), respectively. Under the conditions studied, no metabolic products other than CO(2) and biomass were identified. For xylitol production, fourfold and sixfold concentrated hydrolysate-based media were used to assess the supplementation effects. The type of nutrient supplementation modulated the ratio of total polyols/total extracellular metabolites as well as the xylitol/arabitol ratio. While the former varied from 0.8 to 1, the xylitol/arabitol ratio reached a maximum value of 2.6 for yeast extract (YE)-supplemented hydrolysates. The increase in xylitol productivity and yield was related to the increase of the percentage of consumed xylose induced by supplementation. The best xylitol yield and productivity were found for YE supplementation corresponding to 0.55 g g(-1) and 0.36 g l(-1 )h(-1), respectively. In sixfold concentrated hydrolysates, providing that the hydrolysate was supplemented, the levels of xylitol produced were similar or higher than those for arabitol. Xylitol yield exhibited a further increase in the sixfold hydrolysate supplemented with trace elements, vitamins and minerals to 0.65 g g(-1), albeit the xylitol productivity was somewhat lower. The effect of using activated charcoal detoxification in non-supplemented versus supplemented sixfold hydrolysates was also studied. Detoxification did not improve polyols formation, suggesting that the hemicellulose-derived inhibitor levels present in concentrated BSG hydrolysates are well tolerated by D. hansenii.

Inbreeding in the greater white-toothed shrew, Crocidura russula.

We combined mark-and-recapture studies with genetic techniques of parentage assignment to evaluate the interactions between mating, dispersal, and inbreeding, in a free-ranging population of Crocidura russula. We found a pattern of limited and female-biased dispersal, followed by random mating within individual neighborhoods. This results in significant inbreeding at the population level: mating among relatives occurs more often than random, and F(IT) analyses reveal significant deficits in heterozygotes. However, related mating partners were not less fecund, and inbred offspring had no lower lifetime reproductive output. Power analyses show these negative results to be quite robust. Absence of phenotypic evidence of inbreeding depression might result from a history of purging: local populations are small and undergo disequilibrium gene dynamics. Dispersal is likely caused by local saturation and (re)colonization of empty breeding sites, rather than inbreeding avoidance.

A physiological and enzymatic study of Debaryomyces hansenii growth on xylose- and oxygen-limited chemostats.

The effect of changing growth rate and oxygen transfer rate (OTR) on Debaryomyces hansenii physiology was studied using xylose-limited and oxygen-limited chemostat cultures, respectively, and complemented with enzymatic assays. Under xylose-limited chemostat (oxygen-excess), neither ethanol nor xylitol was produced over the entire range of dilution rate ( D). The maximal volumetric biomass productivity was 2.5 g x l(-1) x h(-1) at D =0.25 h(-1) and cell yield was constant at all values of D. The respiratory rates and xylose consumption rate increased linearly with growth rate but, above 0.17 h(-1), oxygen consumption rate had a steeper increase compared to carbon dioxide production rate. Enzymatic analysis of xylose metabolism suggests that internal fluxes are redirected as a function of growth rate. For values of D up to 0.17 h(-1), the xylose reductase (XR) titre is lower than the xylitol dehydrogenase (XDH) titre, whereas above 0.17 h(-1) XR activity is about twice that of XDH and the NADPH-producing enzymes sharply increase their titres indicating an internal metabolic flux shift to meet higher NADPH metabolic requirements. Moreover, the enzymes around the pyruvate node also exhibited different patterns if D was above or below 0.17 h(-1). Under oxygen-limited chemostat (xylose-excess) the metabolism changed drastically and, due to oxidative phosphorylation limitation, cell yield decreased to 0.16 g g(-1) for an OTR of 1.4 mmol l(-1) h(-1) and xylitol became the major extracellular product along with minor amounts of glycerol. The enzymatic analysis revealed that isocitrate dehydrogenase is not regulated by oxygen, whereas XR, XDH and the NADPH-producing enzymes changed their levels according to oxygen availability.

Variation in mandible shape in Thrichomys apereoides (Mammalia: Rodentia): geometric analysis of a complex morphological structure.

The model of development and evolution of complex morphological structures conceived by Atchley and Hall in 1991 (Biol. Rev. 66:101-157), which establishes that changes at the macroscopic, morphogenetic level can be statistically detected as variation in skeletal units at distinct scales, was applied in combination with the formalism of geometric morphometrics to study variation in mandible shape among populations of the rodent species Thrichomys apereoides. The thin-plate spline technique produced geometric descriptors of shape derived from anatomical landmarks in the mandible, which we used with graphical and inferential approaches to partition the contribution of global and localized components to the observed differentiation in mandible shape. A major pattern of morphological differentiation in T. apereoides is attributable to localized components of shape at smaller geometric scales associated with specific morphogenetic units of the mandible. On the other hand, a clinical trend of variation is associated primarily with localized components of shape at larger geometric scales. Morphogenetic mechanisms assumed to be operating to produce the observed differentiation in the specific units of the mandible include mesenchymal condensation differentiation, muscle hypertrophy, and tooth growth. Perspectives for the application of models of morphological evolution and geometric morphometrics to morphologically based systematic biology are considered.

A cytochrome c peroxidase from Pseudomonas nautica 617 active at high ionic strength: expression, purification and characterization.

Cytochrome c peroxidase was expressed in cells of Pseudomonas nautica strain 617 grown under microaerophilic conditions. The 36.5 kDa dihaemic enzyme was purified to electrophoretic homogeneity in three chromatographic steps. N-terminal sequence comparison showed that the Ps. nautica enzyme exhibits a high similarity with the corresponding proteins from Paracoccus denitrificans and Pseudomonas aeruginosa. UV-visible spectra confirm calcium activation of the enzyme through spin state transition of the peroxidatic haem. Monohaemic cytochrome c(552) from Ps. nautica was identified as the physiological electron donor, with a half-saturating concentration of 122 microM and allowing a maximal catalytic centre activity of 116,000 min(-1). Using this cytochrome the enzyme retained the same activity even at high ionic strength. There are indications that the interactions between the two redox partners are mainly hydrophobic in nature.

Controle de qualidade externo do eritrograma: uma experiência em laboratórios de análises clínicas do Estado do Paraná / Quality control in hematology: an experience in clinical laboratories of Paraná State

Em experiência piloto de controle de qualidade externo para os valores do eritrograma, foi distribuída amostra constituída de eritrócitos humanos suspensos em meio de conservação (LEONART et all, Rev. Bras. Anál. Clín., 21:111-3, 1989) a 10 laboratórios de análises clínicas da Secretaria de Saúde do Estado do Paraná e a 4 outros laboratórios conceituados, incluindo os do Curso de Farmácia e do Hospital de Clínicas da Universidade Federal do Paraná. Os profissionais dos laboratórios participantes empregaram diferentes metodologias para a determinação de eritrócitos, hemoglobina, volume globular, volume corpuscular médio, hemoglobina corpuscular média e concentração de hemoglobina corpuscular média, seguindo instruções preconizadas para o manuseio da amostra. Os laboratórios demonstraram bom desempenho e os dados obtidos tiveram boa reprodutibilidade intra e interlaboratoriais. Estes resultados sugerem a viabilidade da amostra e do sistema empregado para implantação em Programas de Controle de Qualidade Interlaboratoriais.