[Effect of the antioxidant, 4-methyl-2,6 di-tert-butylphenol on the mitotic division of epithelial cells of the small-intestinal crypts of mice depending on the degree of differentiation]. / Vliianie antioksidanta 4-metil-2,6-ditretbutilfenola na mitoticheskoe delenie epitelial'nykh kletok krypt tonkoi kishki myshei v zavisimosti ot stepeni differentsirovki.

Antioxidants increase the number of mitoses in epithelial cell populations being on differentiation, examined from various parts of small intestinal crypts of mice. 2 hours after antioxidant injection a considerable increase in mitotic index (MI) is found, with maximal value (by 3 times) seen for cells from higher crypt region where more differentiated cells occur. The increase of MI is observed also in later period, 14, 21--24 hours after antioxidant administration. The observed effect may be explained by the influence of antioxidants on the reserve pool of cells. However, it does not seem unlikely that the increase of MI after antioxidant injection may be due to the influence of antioxidants on the longevity of individual stages of mitotic cycle. Similar evidence was provided earlier by the authors for the effect of antioxidants on the mitotic division of liver cells of normal mice.

[Effect of an antioxidant (4-methyl-2,6-ditertburylphenol) on mitotic division of mouse liver cells]. / Vliianie antioksidanta (4-metil-2,6-ditretbutilfenola) na mitoticheskoe delenie kletok pecheni myshei.

The highest increase in the mitotic index (MI) reticuloendothelial cells observed in the experiment after antioxidant treatment coincided in time with the maximal MI value of these cells in the untreated animals. However, the maximal increase of MI in hepatocytes took place when the minimal value of MI was registered in cells of the control animals. A significant increase in MI occurred both in hepatocytes and reticuloendothelial cells within the two first hours after antioxidant injection, but at this very time a more evident stimulation was observed for the hepatocytes. Antioxidant doses stimulating cell division produce similar effect on reticuloendothelial cells and hepatocytes, just reported here, as do tireoid ans steroid hormones.