RESUMO
We describe a computational light-sheet microscope designed for hyperspectral acquisition at high spectral resolution. The fluorescence light emitted from the full field-of-view is focused along the entrance slit of an imaging spectrometer using a cylindrical lens. To acquire the spatial dimension orthogonal to the slit of the spectrometer, we propose to illuminate the specimen with a sequence of structured light patterns and to solve the image reconstruction problem. Beam shaping is obtained simply using a digital micromirror device in conjunction with a traditional selective plane illumination microscopy setup. We demonstrate the feasibility of this method and report the first results in vivo in hydra specimens labeled using two fluorophores.
RESUMO
Tunable femtosecond light sources in the short wave and middle wave infrared regions are of utmost importance for various applications ranging from multiphoton microscopy, mid-infrared supercontinuum generation to high-harmonic generation. We report on an all-fusion-spliced fiber laser emitting 80 fs pulses up to 2.9 µm with 35 kW peak power. The laser is based on Raman self-frequency shift effect from 1560 nm up to 3000 nm in germanium-doped fibers fabricated by the widespread modified chemical vapor deposition process.
RESUMO
A novel hyperspectral single pixel system was used to compare different compressive basis patterns for intensity imaging, lifetime imaging, and FRET quantification. Six popular basis patterns were compared experimentally in a phantom containing two fluorescent dyes. The basis patterns that performed best for lifetime quantification were used to measure FRET occurrence in well-plate samples with varying acceptor-donor ratios. The ABS-WP approach using Haar patterns and the compressive sensing approach with Hadamard Ranked patterns displayed the best overall performances at a 50% compression ratio.
