[New principles for obtaining protective antigens from deep cutlures of enterobacteria]. / Novyi printsip polucheniia protektivnykh antigenov iz glubinnykh kul'tur énterobakterii

A model of S. typhi submerged culture was used; application of the principle of sparing extraction of surface protective antigens by the action of sublethal concentrations of two damaging agents differing by the mechanism of inhibition of the processes of vital activity of the microorganisms was founded. The principle is based on regularities characteristic of cells of bacterial pupulations: a) nonhomogeneity of individual cells of the population by the resistance to the damaging agents, and b) independence of distribution in the population cells of resistance to different damaging agents. The following typhoid antigens were obtained by combined action of sublethal concentrations of hydrogen peroxide (0.25%) and formalin (0.1%): antigens with a full antigenic structure (inducing formation of O-, Vi- and H-antibodies), of low toxicity (V50 - 0.65+/-19 mg) and highly immunogenic (AU50- 0.45+/-0.25 mug) in experiments on mice.

[Reactivity and immunogenicity of the Sonne dysentery detergent monovaccine]. / Reaktogennost' i immunogennost' dizenteriinoi detergentnoi monovaktsiny Zonne

The authors elaborated a method of isolation under industrial conditions of Shigella sonnei surface antigens by the treatment of the culture with a detergent (sodium laurylsulfate). The antigens obtained possessed a much lesser toxicity in comparison with the tryptic ones. A method of preparation of a detergent Sonne monovaccine with an increased antigen content (1.3 mg/ml) was described. Experimental series of the preparation were tested on 38 volunteers. A lower reactogenicity of the detergent vaccine (in comparison with the tryptic) and its marked immunological efficacy was demonstrated.

[Construction of new types of chemical vaccines for the prevention of intestinal infections, and their qualitative assessment. IV. Physical properties and immunological characteristics of the experimental series of chemical vaccines presented for comparative study]. / Konstruirovanie noykh tipov khimicheskikh vaktsin dlia profilaktiki kishechnykh infektsii i ikh kachestvennaia otsenka. Soobshchenie IV. Fizicheskie svoistva i immunollgicheskaia kharakteristika eksperimental'nykh serii khimicheskikh vaktsin, predstavlennykh dlia sravnitel'nogo izucheniia

The authors present the results of study of physico-chemical properties of the chemical sorbed monovaccines from the antigens of the causative agents of typhoid fever, paratyphoid A and B and Sonne and Flexner dysentery obtained by various methods; the results of investigation of their reactogenic properties and immunological activity of limited groups of volunteers also given. The vaccines from the antigens obtained by sparing methods were less reactogenic than the rest experimental and control vaccines obtained from the tryptic antigens. The majority of the experimental vaccines caused a significant increase of the specific antibody titres in the sera of the vaccinated persons: typhoid vaccines--of the O-, VI- and H-antibodies, paratyphoid B vaccines--of the O- and H-antibodies; the control vaccines from the tryptic antigens failed to induce the H-antibody synteisis in the vaccinated persons. Experimental dysentery monovaccines induced a greater increase in the antibody titres than the control vaccines.