In-Orbit Attitude Determination of the UVSQ-SAT CubeSat Using TRIAD and MEKF Methods.

Ultraviolet and infrared sensors at high quantum efficiency on-board a small satellite (UVSQ-SAT) is a CubeSat dedicated to the observation of the Earth and the Sun. This satellite has been in orbit since January 2021. It measures the Earth's outgoing shortwave and longwave radiations. The satellite does not have an active pointing system. To improve the accuracy of the Earth's radiative measurements and to resolve spatio-temporal fluctuations as much as possible, it is necessary to have a good knowledge of the attitude of the UVSQ-SAT CubeSat. The attitude determination of small satellites remains a challenge, and UVSQ-SAT represents a real and unique example to date for testing and validating different methods to improve the in-orbit attitude determination of a CubeSat. This paper presents the flight results of the UVSQ-SAT's attitude determination. The Tri-Axial Attitude Determination (TRIAD) method was used, which represents one of the simplest solutions to the spacecraft attitude determination problem. Another method based on the Multiplicative Extended Kalman Filter (MEKF) was used to improve the results obtained with the TRIAD method. In sunlight, the CubeSat attitude is determined at an accuracy better than 3° (at one σ) for both methods. During eclipses, the accuracy of the TRIAD method is 14°, while it reaches 10° (at one σ) for the recursive MEKF method. Many future satellites could benefit from these studies in order to validate methods and configurations before launch.

Processing environment monitoring in low moisture food production facilities: Are we looking for the right microorganisms?

Processing environment monitoring is gaining increasing importance in the context of food safety management plans/HACCP programs, since past outbreaks have shown the relevance of the environment as contamination pathway, therefore requiring to ensure the safety of products. However, there are still many open questions and a lack of clarity on how to set up a meaningful program, which would provide early warnings of potential product contamination. Therefore, the current paper aims to summarize and evaluate existing scientific information on outbreaks, relevant pathogens in low moisture foods, and knowledge on indicators, including their contribution to a "clean" environment capable of limiting the spread of pathogens in dry production environments. This paper also outlines the essential elements of a processing environment monitoring program thereby supporting the design and implementation of better programs focusing on the relevant microorganisms. This guidance document is intended to help industry and regulators focus and set up targeted processing environment monitoring programs depending on their purpose, and therefore provide the essential elements needed to improve food safety.

Detection of non-O157 Shiga toxin-producing Escherichia coli in 375 grams of beef trim enrichments across multiple commercial PCR detection platforms.

Although serotype O157:H7 remains the pathogenic Shiga toxin-producing Escherichia coli (STEC) of primary concern worldwide, some focus in the United States has shifted to six particular non-O157 STEC serogroups (O26, O45, O103, O111, O121, and O145). Some of these serogroups have also emerged as concerns elsewhere around the world, including Europe. The objective of this work was to compare commercial detection methods with the U.S. Department of Agriculture (USDA) reference method for detection of non-O157 STEC in 375 g of beef trim using a limit of detection study design. Overall, the commercial platforms performed well, showing similar levels of sensitivity for detection of presumptive positives for O45, O26, O103, and O121 (PCR screen results only). For O111, one method that utilizes an integrated immunomagnetic separation and PCR approach was more sensitive than a PCR-only screen approach. Additionally, one commercial method showed more presumptive and confirmed positives overall. Use of an immunomagnetic separation tool, such as antibody-coated beads, aided considerably with the confirmation procedures and is an important step when confirming suspect samples. A secondary goal of this study was to evaluate isolation and International Organization for Standardization confirmation protocols used in Europe compared with strategies provided by the USDA Microbiology Laboratory Guidebook (MLG). Generally, results from the USDA confirmation plates (modified Rainbow agar) were better than the European Union confirmation plates (MacConkey agar with or without rhamnose). In summary, detection of non-O157 STEC in 375 g of beef trim can be performed by any of the three methods on the market evaluated in the study.