RESUMO
Tobacco plants, heterozygous for two independent loci involved in the chlorophyll parenchyma differentiation, allow the genotoxic effects of the atmosphere of the industrial estate South of Toulouse to be estimated. Somatic spots of green cellular colonies on yellow-green background, were counted to calculate the cellular rates of reversion. Two experiments were carried out in 1981, and in 1997. A general decrease of genotoxic effects was observed. These observations were interpreted as being due to a general decrease of the air pollution evaluated by the development of the concentrations of three toxic gases before and after the implementation of cleanup devices. The results obtained demonstrate the efficiency of this bio-indicator, which is easy to use and capable of integrating, in situ, genotoxic variations throughout the duration of plants' growth.
