[The effect of hydrodynamic conditions on the interferon-synthesizing activity of swine splenocytes in the production of alpha-interferon]. / Vliianie gidrodinamicheskikh uslovii na interferonsinteziruiushchuiu aktivnost' splenotsitov svinei pri poluchenii al'fa-interferona.

Parameters of physical constituents of the agitation process and mass-exchange characteristics of the suggested bioreactor for suspension cultivation of pig splenocytes are determined. The effect of hydrodynamic conditions on viability and interferon-synthesizing activity of cells in production of alpha-interferon of pigs has been studied. The relation existing between interferonogenesis processes proceeding under suspension and under stationary biosynthesis has been analyzed by the pair correlation method.

[The isolation and purification of porcine interferon-gamma]. / Poluchenie i ochistka svinogo gamma-interferona.

Porcine immune interferon (IF-gamma) was generated by stimulation with bacterial lectins. Physicochemical analysis showed porcine IF-gamma to be a glycoprotein with an isoelectric point around 8.4-8.8 and an apparent molecular weight of 28000 to 30000. A purification process used in this work included sequential steps of precipitation with ammonium sulphate and chromatographic separation on GPG-glass, con A-Sepharose and biogel P-100. This purification process gave a preparation of IF-gamma with specific activity of around 7 x 10(4) units per mg of protein.