Protein structure of the venom in nine species of snake: from bio-compounds to possible healing agents.

Due to its various structures in bio-compounds, snake venom is the indisputable result of evolutionary stages of molecules with an increasingly complex structure, high specificity, and of great importance for medicine because of their potential. The present study proposed an underpinning examination of venom composition from nine species of venomous snakes using a useful and replicable methodology. The objective was the extension of the evaluation of protein fractions in the field up to 230 kDa to permit possible identification of some fractions that are insufficiently studied. The gel capillary electrophoresis method on the chip was performed using an Agilent 2100 bioassay with the 80 and 230-LabChip Protein kits. Interpretation of electrophoresis was performed using the Protein 2100 expert (Agilent) test software as follows: a) Protein 80 (peak size scale): 1.60, 3.5, 6.50, 15.00, 28.00, 46.00, 63.00, 95.00 kDa; b) Protein 230 (peak size scale): 4.50, 7.00, 15.00, 28.00, 46.00, 63.00, 95.00, 150.00, 240.00 kDa. The screening revealed the presence of compounds with a molecular weight greater than 80 kDa, in the case of Vipera aspis and Vipera xantina palestinae. For V. aspis, a 125 kDa molecular weight pro-coagulant protein was identified, known as being involved in the reduction of plasma clotting time without any direct activity in the fibrinogen coagulation process. The samples examined on the Protein 230-LabChip electrophoresis chip can be considered as a novelty with possible uses in medicine, requiring further approaches by advanced proteomics techniques to confirm the intimate structural features and biological properties of snake venoms.

Protein structure of the venom in nine species of snake: from bio-compounds to possible healing agents

Due to its various structures in bio-compounds, snake venom is the indisputable result of evolutionary stages of molecules with an increasingly complex structure, high specificity, and of great importance for medicine because of their potential. The present study proposed an underpinning examination of venom composition from nine species of venomous snakes using a useful and replicable methodology. The objective was the extension of the evaluation of protein fractions in the field up to 230 kDa to permit possible identification of some fractions that are insufficiently studied. The gel capillary electrophoresis method on the chip was performed using an Agilent 2100 bioassay with the 80 and 230-LabChip Protein kits. Interpretation of electrophoresis was performed using the Protein 2100 expert (Agilent) test software as follows: a) Protein 80 (peak size scale): 1.60, 3.5, 6.50, 15.00, 28.00, 46.00, 63.00, 95.00 kDa; b) Protein 230 (peak size scale): 4.50, 7.00, 15.00, 28.00, 46.00, 63.00, 95.00, 150.00, 240.00 kDa. The screening revealed the presence of compounds with a molecular weight greater than 80 kDa, in the case of Vipera aspis and Vipera xantina palestinae. For V. aspis, a 125 kDa molecular weight pro-coagulant protein was identified, known as being involved in the reduction of plasma clotting time without any direct activity in the fibrinogen coagulation process. The samples examined on the Protein 230-LabChip electrophoresis chip can be considered as a novelty with possible uses in medicine, requiring further approaches by advanced proteomics techniques to confirm the intimate structural features and biological properties of snake venoms.

Cloud Quantum Computing of an Atomic Nucleus.

We report a quantum simulation of the deuteron binding energy on quantum processors accessed via cloud servers. We use a Hamiltonian from pionless effective field theory at leading order. We design a low-depth version of the unitary coupled-cluster ansatz, use the variational quantum eigensolver algorithm, and compute the binding energy to within a few percent. Our work is the first step towards scalable nuclear structure computations on a quantum processor via the cloud, and it sheds light on how to map scientific computing applications onto nascent quantum devices.

Efficiency of four currently used decontamination conditionings in Romania against Aspergillus and Candida strains.

INTRODUCTION: Efficacy of four commercial biocidal products (noted A to D), using manufacturers' recommendations, and a contact time of 30minutes, were evaluated in the purpose of standard SR EN1657: 2006 adapted. METHODS: Were used four strains, two as reference: Aspergillus brasiliensis (niger) (ATCC 16404) and Candida albicans (ATCC 10231), and two isolates: Aspergillus flavus and respectively Aspergillus fumigatus. The inoculum plates containing Malt Extract Agar (MEA) were incubated 48h for C. albicans and 72hours for Aspergillus spp. The standard SR EN1657: 2006 adapted was conducted in two phases: the test cultures preparation and the method validation. Method validation included: the control of experimental conditions and of neutralizant solution, and the method verification. RESULTS: Results revealed that three from the four tested products (A, B and D) had exerted biocidal effect on the studied strains at the recommended concentrations, the registered CFU values being reduced by more than 4 log10, conversely in the case of the product (C), applied against A. fumigatus at the recommended concentration of 2%, the biocidal effect was not detected, fact confirmed also by the CFU's value (3.59 log10). The biocide retested at a greater concentration (of 5%), showed a biocidal effect against A. fumigatus after 30minutes, the CFU value being reduced, by more than 5.29 log10, evidencing the resistance emergence of A. fumigatus under the repeated pressure of biocides. CONCLUSION: It is re-confirming that merely the "chemical" defense measures to defuse the fungi's strategies become unproductive.

Surveys on therapeutic effects of "halotherapy chamber with artificial salt-mine environment" on patients with certain chronic allergenic respiratory pathologies and infectious-inflammatory pathologies.

Halotherapy (HT), derived from speleotherapy in salt mines, is also a drug-free therapeutic method. HT effects vary depending on the therapeutic method and the structure of halotherapy environment. The purpose of this article is to show the HT effects of "halotherapy chamber with artificial salt-mine environment" of the National Institute of Rehabilitation, Physical Medicine and Balneoclimatology (INRMFB), on patients with bronchial asthma and other chronic, infectious-inflammatory and allergic respiratory diseases, describing the clinical effects on certain nonspecific resistance factors, on markers of inflammatory processes and on certain immunological changes. Patients were clinically assessed, with the application of hematologic investigations, analysis of nonspecific resistance to infection and of inflammatory process markers, immunologic assessments, analysis of sodium and potassium concentrations, of mineralocorticoid function and other biochemical tests. For the experimental HT therapy performed in the "halotherapy chamber with artificial salt-mine environment" of INRMFB, 15 patients suffering from bronchial asthma, allergic rhinitis, chronic bronchitis, chronic obstructive bronchopneumopathy were selected, based on specific medical indications and contraindications and applying ethical principles, as well as 4 patients with similar pathologies for the control group, who underwent in-home drug treatment. After the specific halotherapy treatment on patients with bronchial asthma, chronic bronchitis and chronic obstructive bronchopneumopathy, which also showed other chronic, infectious-inflammatory and allergic respiratory pathologies, triggering of anti-inflammatory (and also anti allergic) mechanisms and healing effects on inflammatory process were noted. Data acquired also proved the halo therapeutic effect causing the reduction of sensitiveness of body in patients with bronchial asthma.

Hole-doped semiconductor nanowire on top of an s-wave superconductor: a new and experimentally accessible system for Majorana fermions.

Majorana fermions were envisioned by Majorana in 1935 to describe neutrinos. Recently, it has been shown that they can be realized even in a class of electron-doped semiconductors, on which ordinary s-wave superconductivity is proximity induced, provided the time reversal symmetry is broken by an external Zeeman field above a threshold. Here we show that in a hole-doped semiconductor nanowire the threshold Zeeman field for Majorana fermions can be very small for some magic values of the hole density. In contrast to the electron-doped systems, smaller Zeeman fields and much stronger spin-orbit coupling and effective mass of holes allow the hole-doped systems to support Majorana fermions in a parameter regime which is routinely realized in current experiments.

[Conditioning factors in the quality of health education for workers in public and collective food catering services]. / Facteurs de conditionnement de la qualité de l'acte éducationnel chez les travailleurs des unités d'alimentation publique et collective.

The authors started from the assumption that "expectations" relating to hygienic work habits of workers in the food catering industry were a function of their health education. Research was conducted in three major food catering units both before and after completion of health education courses. In order to assess workers' awareness, they were asked to fill in a questionnaire pertaining to their sanitary information and their approaches to washing up, cleaning and disinfection. Samples taken from tableware, tools and workers' hands were tested microbiologically. Indices computed to measure the efficiency of the health education courses and the standards of microbial safety, as well as the chi 2 test confirmed the non-stochastic character of the positive findings in experimental units vs. controls.

Study on free and total urinary hydroxyproline in old men.

The present study deals with urinary free and total hydroxyproline (HOP) in a group of adults between 63-93 years old, admitted in a sanatorium for geriatries. The average value of the free HOP on the whole group was of 1.28 mg/24 hours (0.52-2.10) and that of the total HOP was of 16.54 mg/24 hours (10.7-27.94). The free HOP does not show differences as compared to the adults; but the total one decreases in old people. The data obtained are discussed and compared with the literature.