Assuntos
Aciclovir/farmacologia , Antibióticos Antineoplásicos/farmacologia , Antimetabólitos/farmacologia , Herpesvirus Humano 1/efeitos dos fármacos , Herpesvirus Humano 2/efeitos dos fármacos , Ácido Micofenólico/farmacologia , Efeito Citopatogênico Viral/efeitos dos fármacos , Sinergismo Farmacológico , Humanos , Replicação Viral/efeitos dos fármacosRESUMO
The interferon reaction of leucocytes (IRL) in the newborns and their mothers were examined in vitro. The ability of leucocytes, obtained from the blood of the umbilical cord of newborns, to synthesize interferon was established in 48.78% of the investigated newborns while in their mothers the relative part was 70.73%. The mean geometrical titre of the leucocytic interferon was lower than that of the parturients (30.90-1.02 against 41.69-1.70).
Assuntos
Sangue Fetal/imunologia , Interferon Tipo I/sangue , Leucócitos/imunologia , Adulto , Células Cultivadas , Feminino , Humanos , Imunidade Inata/imunologia , Recém-Nascido , Valores de ReferênciaRESUMO
DNA free antiherpes subunit vaccines were prepared from diploid human embryonic lung cells infected either with type 1 or type 2 herpes simplex viruses (HSV). Virion and membrane-bound virus-specific glycoproteins were solubilized with Nonidet P-40 and separated by ultracentrifugation. The antigenic properties of the vaccine were tested in guinea pigs. Antibody response was followed by virus neutralization and complement fixation. The vaccine itself was low-immunogenic, however its immunogenicity has considerably increased by usage of suitable adjuvants. In virus neutralization test higher antibody titre was found against homologous virus. The antibody response was related to protein content and to the frequency of vaccination.
Assuntos
Anticorpos Antivirais/biossíntese , Simplexvirus/imunologia , Vacinas Virais/imunologia , Adjuvantes Imunológicos , Animais , Antígenos de Superfície/imunologia , Antígenos Virais/imunologia , Testes de Fixação de Complemento , DNA Viral/isolamento & purificação , Fibroblastos , Cobaias , Humanos , Testes de Neutralização , Simplexvirus/genéticaRESUMO
The thymidine kinase inducing ability of 104 strains of herpes simplex virus was studied comparatively. A pronounced relationship was established between induction of the enzyme and the serotype of the strains. As a rule, the strains of serotype 2 are weaker inducer of dThd- and dCyd-kinase activity than serotype 1 strains. A certain parallelism exists between induction of both enzymes, however the activity of the thymidine kinase increases after infection with herpes simplex virus 4--5 times more than that of the dCyd-kinase. Adaptation of the strains to cell cultures only slightly modifies the inducing ability of the herpes simplex virus strains. The thymidine kinase activity induced by HSV-1 and HSV-2 differ from each other and are different from the cell enzyme with respect to their thermal stability at 40 degrees C. These differences are expressed more clearly in the presence of 480 muM dThdMP during inactivation. dThdMP stabilizes the type 1 but not the type 2 enzyme.
