RESUMO
A brief survey of gas and liquid chromatographic methods for analysis, purification and quantitation of natural insect juvenile hormones and one commercially used analoh, altosid (methoprene) insect growth regulator, includes references to the most recent literature.
Assuntos
Cromatografia Gasosa , Hormônios Juvenis/análise , Metoprene , Especificidade da Espécie , Relação Estrutura-AtividadeRESUMO
Residues of isopropyl (2E,4E)-11-methoxy-3,7,11-trimethyl-2,4-dodecadienoate (Altosid) insect growth regulator are determined in waters, soils, plants, milk, eggs, fish, shellfish, poultry and cattle tissues, blood, urine, and feces. Acetonitrile is the primary extraction solvent for all samples. Residues are extracted by high-speed blending followed by vacuum filtration. Fatty extracts are subjected to cold-temperature precipitation and filtration. Samples are cleaned up by petroleum ether partitioning and Florisil and neutral alumina chromatography. The concentrated eluants are analyzed by gas-liquid chromatography (GLC) on columns of differing polarity, using hydrogen flame ionization detectors. The identity of suspected residues is confirmed by additional GLC and by mass fragmentography. The lower limits of detection were: water samples, 0.0004-0.001 ppm; soils, blood, and urine, 0.001 ppm; forage grasses, forage legumes, and rice foliage, 0.005 ppm; and milk, eggs, fish, shellfish, poultry and cattle tissues, and feces, 0.010 ppm.
Assuntos
Poluentes Ambientais/análise , Acetonitrilas , Animais , Bovinos , Cromatografia Gasosa , Ovos/análise , Fezes/análise , Produtos Pesqueiros/análise , Espectrometria de Massas , Métodos , Metoprene/sangue , Metoprene/urina , Leite/análise , Resíduos de Praguicidas/análise , Extratos Vegetais/análise , Produtos Avícolas/análise , Solo/análise , Extratos de Tecidos/análise , Poluentes da Água/análiseRESUMO
Two juvenile hormones are isolated from organ cultures of corpora allata of the tobacco hornworm moth, Manduca sexta Johannson, and are purified by high-resolution liquid chromatography. These are identified as methyl (2E, 6E)-(10R)-10,11-epoxy-3,7,11-trimethyl-2,6-dodecadienoate, a new natural hormone, and methyl (2E,6E) - (10R,11S) - 10,11 - epoxy - 3,7,11 - trimethyl-2,6-tridecadienoate. [(14)C]Methionine is incorporated only into their methoxycarbonyl group. Details of the in vitro techniques and the chemical proof of structures are presented. The significance of the occurrence of a new juvenile hormone and the new techniques used for production, isolation, and identification are discussed.
