Histological findings in equine testes one year after standing laparoscopic peritoneal flap hernioplasty.

In order to know reproduction-related complications due to standing laparoscopic peritoneal flap hernioplasty, histological characteristics of the testicles from five stallions one year after surgery were compared with seven testicles from four healthy stallions. Moreover, the daily sperm output (DSO) was determined before (T0) and one year after surgery (T12). DSO did not show significant differences between T0 and T12. The diameter of the seminiferous tubules was significantly decreased in the samples from the hernioplasty group. The percentage of tubules with full spermatogenesis was smaller in the hernioplasty group, but the difference was not significant. It can be concluded that standing laparoscopic peritoneal flap hernioplasty produced mild histological changes in the testicular parenchyma, epididymis and pampiniform plexus after one year follow-up.

Development of a technique for standing hand-assisted laparoscopic splenectomy in five horses.

OBJECTIVES: To develop an experimental standing hand-assisted laparoscopic splenectomy (HALS) technique, report the associated peri-operative complications and document the short-term surgical outcomes. METHODS AND RESULTS: Five healthy 300-470 kg horses that underwent standing HALS. Spleens of different weights (2.25-7.0 kg) were removed using this technique. The main complication during surgery was difficulty sectioning the gastrosplenic ligament. The postoperative complications included adhesions of the colon to the nephrosplenic ligament stump and incisional discharge in two horses. CONCLUSIONS: Standing HALS is a feasible experimental procedure for medium-sized horses, which avoids rib excision and general anaesthesia, but requires further development.

Standing laparoscopic peritoneal flap hernioplasty of the vaginal rings does not modify the sperm production and motility characteristics in intact male horses.

Laparoscopic hernioplasty techniques have been developed in the recent years to avoid the recurrence of inguinal hernias and to spare the testicles for breeding purposes in stallions. However, there have been no previous comprehensive and systematic studies of the reproductive outcomes and prognoses for stallions after inguinal hernioplasty. Therefore, the objective of this study was to assess the possible effects of one of these techniques (standing laparoscopic peritoneal flap hernioplasty) on the sperm production and motility characteristics of six healthy stallions that received this procedure based on 1-year follow-ups. There were no significant differences in the measured sperm variables (assessments based on the DSO, MOT, PMOT, VSL, VCL and VAP) during 1-year follow-ups.

Intra and postoperative assessment of re-sterilised Ligasure Atlas for orchidectomies in horses: clinical study.

Horses (n=35) underwent orchidectomy in a single institution with a re-sterilised LSA as sole means of haemostasis. During the surgery, the gross quality of the seal, the stickiness of the forceps to the tissues, bleeding/oozing from the stump and the need for a subsequent application in already severed spermatic cord were assessed for haemostasis quality. After surgery, physical parameters (appearance of the mucous membranes, quality of the peripheral pulse, heart rate, respiratory rate, rectal temperature and blood dripping from the incisions), haematology or packed cell volume and total protein were monitored to assess signs of bleeding or any other condition. For cleanliness and asepsis assessment, signs of surgical infection were recorded. Complications during surgery were mild degree of sticking of the LSA forceps to the tissues and dulling of the blade. There was no need to reapply LSA a second time except in one horse. This means a haemostasis complication rate of 2.85 per cent of the horses. No postoperative bleeding was detected. Only two horses with fever had associated signs of surgical site infection. This means an infection rate of 5.71 per cent of the horses.

Lymphoma in a rabbit: histopathological and immunohistochemical findings.

Lymphoma is a very common lymphoid neoplasm in domestic animals, but few naturally occurring cases have been reported in rabbits. It presents at different sites within rabbits and, although the macroscopic pattern tends to be similar, different cell populations may be involved. This report describes a case of spontaneous lymphoma ocurring in a two-and-a-half-year-old pet Dutch dwarf rabbit. T and B lymphocyte infiltrates were observed in skin, lung, kidney, liver, intestine and lymph nodes, in each case affecting one or more tissue structures. The diagnosis, based on microscopic and immunocytochemical findings, was multicentric, T cell-rich B cell lymphoma with skin involvement.

Diagnostico citológico de la microaspiración alimentaria / Cytodiagnosis of food microaspiration

Durante 6 meses, de mayo a octubre de 1984, se estudiaron 64 lactantes de 4 a 24 meses de edad con broncopatía obstructiva recidivante (BOR) y un grupo control de 10 niños sanos con el objeto de evaluar en ellos la incidencia del fenómeno microaspirativo mediante un método citológico. Este método diagnostica etiológicamente la microaspiración mediante la determinación de elementos grasos tingibles fagocitados por los macrófagos alveolares y puestos de manifiesto por dos parámetros correlativos: a) un nuevo método de tinción celular con adecuada fidelidad morfológica, y b) una mayor cantidad de muestras y extendidos exploratorios de cada paciente, permitiendo, de este modo, una eliminación más fácil de falsos positivos y mejor individualización de falsos negativos. Los datos obtenidos por medio de la técnica fueron procesados estadísticamente con la prueba de significación de chi cuadrado (X**2) con el objeto de realizar un estudio exploratorio-descriptivo del método, mediante la comparación de las puntuaciones medias obtenidas por nuestras, extendidos y macrófagos. Sus resultados son altamente satisfactorios con rechazo de la hipótesis nula a un nivel de confianza entre el 98,6% según se consideren muestras o extendidos respectivamente. De los 64 pacientes se encontró microaspiración en 24 de ellos (37,5%), lo que denota su alta incidencia en pacientes afectados de BOR. En el grupo control no se registraron fenómenos microaspirativos (AU)

Diagnostico citológico de la microaspiración alimentaria / Cytodiagnosis of food microaspiration

Durante 6 meses, de mayo a octubre de 1984, se estudiaron 64 lactantes de 4 a 24 meses de edad con broncopatía obstructiva recidivante (BOR) y un grupo control de 10 niños sanos con el objeto de evaluar en ellos la incidencia del fenómeno microaspirativo mediante un método citológico. Este método diagnostica etiológicamente la microaspiración mediante la determinación de elementos grasos tingibles fagocitados por los macrófagos alveolares y puestos de manifiesto por dos parámetros correlativos: a) un nuevo método de tinción celular con adecuada fidelidad morfológica, y b) una mayor cantidad de muestras y extendidos exploratorios de cada paciente, permitiendo, de este modo, una eliminación más fácil de falsos positivos y mejor individualización de falsos negativos. Los datos obtenidos por medio de la técnica fueron procesados estadísticamente con la prueba de significación de chi cuadrado (X**2) con el objeto de realizar un estudio exploratorio-descriptivo del método, mediante la comparación de las puntuaciones medias obtenidas por nuestras, extendidos y macrófagos. Sus resultados son altamente satisfactorios con rechazo de la hipótesis nula a un nivel de confianza entre el 98,6% según se consideren muestras o extendidos respectivamente. De los 64 pacientes se encontró microaspiración en 24 de ellos (37,5%), lo que denota su alta incidencia en pacientes afectados de BOR. En el grupo control no se registraron fenómenos microaspirativos