Attitudes towards contact lenses: a comparative study of teenagers and their parents.

PURPOSE: This two-phase survey aimed to identify the beliefs and attitudes that create a barrier towards contact lens (CL) fitting among adolescents (aged 12-18 years) and their parents attending eye care practitioner clinics in Italy (phase 1) and Iberia (Spain and Portugal; phase 2). In phase 2, the sample was further focused, by limiting it to those adolescents who did not already wear CL. METHODS: The extent to which CL satisfy aesthetic, visual, and practical needs and their effectiveness, safety, and comfort in the general population and in adolescents was rated by respondents on a 5-point Likert scale. RESULTS: In phase 1, approximately one-third of adolescents (n=146) and parents (n=114) were CL wearers. Most adolescents (77.5% of 223) and parents (66.2% of 230) expressed a high interest in CL use in phase 2 despite none of the adolescents currently wearing CL. Parents, but not adolescents, perceived that CL were significantly less safe in adolescents than in the general population (p<0.05) in phases 1 and 2. Across both phases, adolescents and parents agreed that CL met an aesthetic need in adolescents (p<0.05 versus general population). Among 50% of parents (mainly mothers), significant concerns regarding CL were difficulties following instructions and taking care of the CL and potential eye damage, which, in mothers, led them to show less agreement towards CL use by the adolescent (p<0.001). CONCLUSIONS: These findings highlight an essential need for improved education on CL use in the population.

Marinobacter lacisalsi sp. nov., a moderately halophilic bacterium isolated from the saline-wetland wildfowl reserve Fuente de Piedra in southern Spain.

A Gram-negative, non-spore-forming, motile, moderately halophilic, aerobic, rod-shaped bacterium, designated strain FP2.5(T), was isolated from the inland hypersaline lake Fuente de Piedra, a saline-wetland wildfowl reserve located in the province of Málaga in southern Spain. Strain FP2.5(T) was subjected to a polyphasic taxonomic study. It produced colonies with a light-yellow pigment. Strain FP2.5(T) grew at salinities of 3-15 % (w/v) and at temperatures of 20-40 degrees C. The pH range for growth was 5-9. Strain FP2.5(T) was able to utilize various organic acids as sole carbon and energy source. Its major fatty acids were C(16 : 0), C(18 : 1)omega9c and C(16 : 1)omega9c. The DNA G+C content was 58.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FP2.5(T) appeared to be a member of the genus Marinobacter and clustered closely with the type strains of Marinobacter segnicrescens, Marinobacter bryozoorum and Marinobacter gudaonensis (levels of 16S rRNA gene sequence similarity of 98.1, 97.4 and 97.2 %, respectively). However, DNA-DNA relatedness between the new isolate and the type strains of its closest related Marinobacter species was low; levels of DNA-DNA relatedness between strain FP2.5(T) and M. segnicrescens LMG 23928(T), M. bryozoorum DSM 15401(T) and M. gudaonensis DSM 18066(T) were 36.3, 32.1 and 24.9 %, respectively. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, strain FP2.5(T) is considered to represent a novel species of the genus Marinobacter, for which the name Marinobacter lacisalsi sp. nov. is proposed. The type strain is FP2.5(T) (=CECT 7297(T)=LMG 24237(T)).