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1.
Bone ; 152: 116088, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34175502

RESUMO

The outcomes of animal experiments can be influenced by a variety of factors. Thus, precise reporting is necessary to provide reliable and reproducible data. Initiatives such as the ARRIVE guidelines have been enrolled during the last decade to provide a road map for sufficient reporting. To understand the sophisticated process of bone regeneration and to develop new therapeutic strategies, small rodents, especially mice, are frequently used in bone healing research. Since many factors might influence the results from those studies, we performed a systematic literature search from 2010 to 2019 to identify studies involving mouse femoral fracture models (stable fixation) and evaluated the reporting of general and model-specific experimental details. 254 pre-selected publications were systematically analyzed, showing a high reporting accuracy for the used mouse strain, the age or developmental stage and sex of mice as well as model-specific information on fixation methods and fracturing procedures. However, reporting was more often insufficient in terms of mouse substrains and genetic backgrounds of genetically modified mice, body weight, hygiene monitoring/immune status of the animal, anesthesia, and analgesia. Consistent and reliable reporting of experimental variables in mouse fracture surgeries will improve scientific quality, enhance animal welfare, and foster translation into the clinic.


Assuntos
Modelos Animais de Doenças , Fraturas do Fêmur , Animais , Regeneração Óssea , Fraturas do Fêmur/diagnóstico por imagem , Humanos , Camundongos , Dor , Manejo da Dor , Publicações Periódicas como Assunto/normas
2.
Sci Rep ; 11(1): 10918, 2021 05 25.
Artigo em Inglês | MEDLINE | ID: mdl-34035397

RESUMO

While the use of local anesthesia as part of multimodal pain management is common practice in human and veterinarian surgery, these drugs are not applied routinely in rodent surgery. Several recommendations on the use of local anesthesia exist, but systematic studies on their efficacy and side effects are lacking. In the present study, male and female C57BL/6J mice were subjected to a sham vasectomy or a sham embryo transfer, respectively. We tested whether a mixture of subcutaneously injected Lidocaine and Bupivacaine in combination with systemic Paracetamol applied via drinking water results in superior pain relief when compared to treatment with local anesthesia or Paracetamol alone. We applied a combination of methods to assess behavioral, emotional, and physiological changes indicative of pain. Voluntary Paracetamol intake via drinking water reached the target dosage of 200 mg/kg in most animals. Local anesthesia did not lead to obvious side effects such as irregular wound healing or systemic disorders. No relevant sex differences were detected in our study. Sevoflurane anesthesia and surgery affected physiological and behavioral measurements. Surprisingly, Paracetamol treatment alone significantly increased the Mouse Grimace Scale. Taken together, mice treated with a combination of local anesthesia and systemic analgesia did not show fewer signs of post-surgical pain or improved recovery compared to animals treated with either local anesthesia or Paracetamol.


Assuntos
Acetaminofen/administração & dosagem , Bupivacaína/administração & dosagem , Transferência Embrionária/efeitos adversos , Lidocaína/administração & dosagem , Vasectomia/efeitos adversos , Acetaminofen/farmacologia , Animais , Comportamento Animal/efeitos dos fármacos , Bupivacaína/farmacologia , Água Potável/administração & dosagem , Água Potável/química , Sinergismo Farmacológico , Feminino , Injeções Subcutâneas , Laparotomia/efeitos adversos , Lidocaína/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Manejo da Dor/métodos , Resultado do Tratamento
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