[Investigation of the prevalence of visceral leishmaniasis by the microculture method and IFAT in dogs in Kirikkale.]. / Kirikkale'deki Köpeklerde Mikrokültür Yöntemi ve IFAT ile Visseral Leishmaniosisin Prevalansinin Arastirilmasi.

This study was carried out in order to compare the seroprevalence of visceral leishmaniasis in dogs from different areas of Kirikkale between 2006-2008 using the microculture method (MCM) which is a new method, and the indirect fluorescent antigen test (IFAT). All of the blood collected from total of 50 dogs was found to be negative by MCM. Only one male Beagle strain dog (3 years old) was found to be seropositive at 1/128 titers (2%) for anti-Leishmania infantum IgG antibodies by IFAT.

The prevalence of Dirofilaria immitis in dogs in Kirikkale.

Heartworm infection is one of the most important parasitic diseases in dogs. The aim of the present study was to determine the prevalence of heartworm in dogs in Kirikkale. Blood samples of 172 dogs were collected. Microfilariae were detected in 10 (5.8%). dogs with the Knott technique. The anal and excretory pores stained with acid phosphates showed that the microfilariae belonged to Dirofilaria immitis. Microfilariae were found in dogs aged between 2-10 years, and especially in Siberian huskies, setters, pointers and crossbred sheepdogs. The serum samples of 142 dogs were analyzed using the commercial ELISA test kit for detection of anti-D. immitis antibody. The occult infection rate was found to be 27.46% in dogs. According to these results, Kirikkale region has a high risk for heartworm infection.