Growth of adenocarcinoma from canine pleural fluid on aerobic bacterial culture.

We report a case of canine adenocarcinoma with multi-organ metastasis in which colonies of adenocarcinoma cells grew upon aerobic bacterial culture of pleural effusion. Stained agar colonies were highly similar to rare suspicious cells seen on cytologic examination of the pleural effusion, as well as rare cells seen on cytologic examination of pancreatic and gastric wall fine-needle aspirates. Cells from colonies growing on agar media were mildly immunoreactive for cytokeratin. Histologic examination of tissues obtained at autopsy revealed pancreatic adenocarcinoma with vascular invasion and nodal, gastric, pulmonary, and pleural metastasis.

Impact of disinfectant-filled foot mats on mechanical transmission of bacteria in a veterinary teaching hospital.

OBJECTIVE: To evaluate the efficacy of disinfectant-filled foot mats at reducing tracking of Salmonella enterica and overall bacterial contamination on floors in a veterinary teaching hospital. DESIGN: Prospective study. Samples-Bacteria collected from floors before and after placement of disinfectant-filled foot mats. PROCEDURES: Foot mats filled with a phenolic-based disinfectant were placed at key transition areas in common-use corridors between the large animal hospital (LAH) and small animal hospital in a veterinary medical teaching hospital. Microbiological samples were collected for total bacterial counts and for the presence of S enterica at 14 designated sample sites in the veterinary medical teaching hospital. Samples were collected at regular intervals for 7 months before mat placement and for 13 months after mat placement. RESULTS: Median numbers of aerobic bacteria isolated before and after disinfectant mat placement were not significantly different for most sites sampled. For 3 of the 4 transition areas between the LAH and connecting common-use corridor, there was a significant difference in median bacterial counts on either side of the threshold. This difference was significant regardless of whether a disinfectant mat was present or not. Salmonella enterica isolates were cultured from several sites in the LAH and sites outside the LAH, irrespective of the presence of a disinfectant mat. CONCLUSIONS AND CLINICAL RELEVANCE: -Disinfectant-filled mats may not be uniformly effective in reducing the bacterial load on floors or in reducing mechanical tracking of S enterica from contaminated areas in a veterinary teaching hospital. Further studies are needed to determine effective measures to reduce mechanical transmission of bacteria on footwear in veterinary hospitals.