RESUMO
In the vertebrate olfactory system, sensory neurons with common odorant specificities project to specific glomeruli in the olfactory bulb. How do olfactory sensory neurons find their glomerular targets? To address this question, we have visualized the genesis of the peripheral olfactory system in living zebrafish embryos. Dye labelings reveal that a primordial yet stereotyped map of glomeruli is apparent during embryogenesis. By labeling a small number of cells with an ectopically expressed green fluorescent protein reporter, we can observe the dynamic growth behaviors of individual olfactory neuron growth cones as they project to their glomeruli. We find that olfactory axons extend directly to their partner glomeruli, suggesting that these cells' growth cones rely upon pathfinding cues to reach their targets.
Assuntos
Neuritos/fisiologia , Bulbo Olfatório/citologia , Bulbo Olfatório/embriologia , Neurônios Receptores Olfatórios/citologia , Vias Aferentes/citologia , Vias Aferentes/embriologia , Vias Aferentes/fisiologia , Animais , Animais Geneticamente Modificados , Axônios/fisiologia , Compostos de Boro , Carbocianinas , Corantes Fluorescentes , Genes Reporter , Proteínas de Fluorescência Verde , Proteínas Luminescentes , Microscopia de Vídeo , Neuritos/química , Plasticidade Neuronal/fisiologia , Bulbo Olfatório/fisiologia , Neurônios Receptores Olfatórios/fisiologia , Neurônios Receptores Olfatórios/ultraestrutura , Plasmídeos , Peixe-ZebraRESUMO
Strain AK127 is a developmental mutant of Dictyostelium discoideum that was isolated by restriction enzyme-mediated integration (REMI). Mutant cells aggregate normally but are unable to proceed past the loose aggregate stage. The cloned gene, lagC (loose aggregate C), encodes a novel protein of 98 kD that contains an amino-terminal signal sequence and a putative carboxy-terminal transmembrane domain. The mutant strain AK127 shows no detectable lagC transcript upon Northern analysis, indicating that the observed phenotype is that of a null allele. Expression of the lagC cDNA in AK127 cells complements the arrest at the loose aggregate stage, indicating that the mutant phenotype results from disruption of the lagC gene. In wild-type cells, lagC mRNA is induced at the loose aggregate stage and is expressed through the remainder of development. lagC- null cells aggregate but then disaggregate and reaggregate to form small granular mounds. Mature spores are produced at an extremely low efficiency (< 0.1% of wild type), appearing only after approximately 72 hr, whereas wild-type strains produce mature spores by 26 hr. lagC- null cells accumulate reduced levels of transcripts for the prestalk-enriched genes rasD and CP2 and do not express the DIF-induced prestalk-specific gene ecmA or the cAMP-induced prespore-specific gene SP60 to significant levels. In chimeric organisms resulting from the coaggregation of lagC- null and wild-type cells, cell-type-specific gene expression is rescued in the lagC- null cells; however, lagC- prespore cells are localized to the posterior of the prespore region and do not form mature spores, suggesting that LagC protein has both no cell-autonomous and cell-autonomous functions. Overexpression of lagC from an actin promoter in both wild-type and lagC- cells causes a delay at the tight aggregate stage, the first stage requiring LagC activity. These results suggest that the LagC protein functions as a nondiffusible cell-cell signaling molecule that is required for multicellular development.
Assuntos
Comunicação Celular/genética , Dictyostelium/crescimento & desenvolvimento , Genes de Protozoários/genética , Proteínas de Membrana/genética , Proteínas de Protozoários , Transdução de Sinais/genética , Actinas/biossíntese , Actinas/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Diferenciação Celular/genética , DNA Complementar/genética , Proteínas de Ligação a DNA/biossíntese , Proteínas de Ligação a DNA/genética , Dictyostelium/citologia , Dictyostelium/genética , Proteínas Fúngicas/biossíntese , Proteínas Fúngicas/genética , Histocitoquímica , Proteínas de Membrana/biossíntese , Dados de Sequência Molecular , Morfogênese/genética , Mosaicismo , Mutação , RNA Mensageiro/biossíntese , Proteínas de Ligação a RNA , Proteínas Recombinantes de Fusão , Análise de Sequência de DNA , Fatores de Transcrição/biossíntese , Fatores de Transcrição/genética , Proteínas ras/biossíntese , Proteínas ras/genéticaRESUMO
We constructed a partial Sau3A Dictyostelium genomic DNA library in a shuttle vector that replicates extrachromosomally in Dictyostelium cells. This library was used to complement Dictyostelium strain HPS400, which lacks thymidylate synthase activity and requires exogenous thymidine for growth. We have used a modified high-frequency transformation protocol that allows the introduction of the library into a sufficient number of Dictyostelium cells to select complementing plasmids. Using this approach, we have isolated a gene (Thy1) that complements the thymidine growth requirement of HPS400. The gene encodes a 1.2-kilobase RNA and the derived amino acid sequence shows no homology to thymidylate synthase, a protein highly conserved throughout evolution, or any other protein sequence in the data base examined. Thy1 provides an important selectable marker for transforming Dictyostelium cells. In addition, this work suggests that it will be possible to isolate genes that are essential for developmental processes in Dictyostelium by complementation.
