[The induction and elimination of cytogenetic disorders in monkey lymphocytes following thiophosphamide action]. / Induktsiia i éliminatsiia tsitogeneticheskikh narushenii v limfotsitakh obez'ian posle vozdeistviia tiofosfamidom.

The dynamics of sister chromatid exchanges and chromosome aberrations in lymphocyte of monkey has been investigated after a thiophosphamide exposure. The process of induction and elimination of cytogenetic damages was described by the mathematical model. Developing the model in detail will allow to make a cytogenetic prognosis of remote consequences of mutagenic exposure.

[Comparison of the cytogenetic effects of cyclophosphamide on monkey lymphocytes in vivo and in vitro]. / Sravnenie tsitogeneticheskikh éffektov pri deistvii tsiklofosfamida na limfotsity obez'ian in vivo i in vitro.

The comparative in vivo and in vitro study of chromosomal aberrations and SCE induced by cyclophosphamide (CP) in macaca rhesus lymphocytes was performed. The dose of mutagenic exposure for quantitative estimation of effects was determined as a product of concentration of alkylating CP metabolites on the exposure time. The mutagenic effect caused by the same doses of CP (CP metabolites) appeared similar in vivo and in vitro. This suggests that the results obtained in adequate in vitro mutagen-testing experiments may be quantitatively extrapolated for the in vivo conditions.

[Incidence of chromosome aberrations in the bone marrow of monkeys and their progeny after exposure to aflatoxin B1]. / Chastota aberratsii khromosom v kostnom mozge obez'ian i ikh potomstva posle vozdeistviia aflatoksinom B1.

A mutagenic effect of aflatoxin B1 (AFB1) was studied in somatic cells of primates. It is shown that AFB1 elevates the chromosome aberration frequency in bone marrow cells of two monkey species: Macaca mulatta and Papio hamadryas. Genome mutations were found in these experimental primates simultaneously with chromosome aberrations. The AFB1-induced chromosome aberrations were revealed at least during two years of the study. The fact of the chromosome aberration frequency increase was established in Macaca babies born from females who received AFB1 in pregnancy.

[Frequency of radiation-induced exchanges in easily identifiable monkey chromosomes]. / Chastota indutsirovannykh radiatsiei obmenov v legko identifitsi-ruemykh khromosomakh obez'ian.

Frequency of asymmetric exchanges of readily identifiable chromosomes 1, 2, 3, 4, 8, 9 ad 20 was studied in primary kidney cell cultures of monkeys. Macaca mulatta and Papio hamadryas irradiated with 200 R of X-rays at G0. The chromosomes studied enter asymmetric exchange in accordance with their lengths of mitosis. The frequency of exchanges is independent of the quantity of late-replicating heterochromation in chromosomes. The radiosensitivity of chromosomes was identical in the two monkey species.

[Comparative study of the radiosensitivity of monkey chromosomes in cellular irradiation in vivo and in vitro]. / Sravnitel'noe izuchenie radiochuvstvitel'nosti khromosom obez'ian pri obluchenii kletok in vivo i in vitro.

Chromosome radiosensitivity was studied in the primary culture of peripheral blood lymphocytes and epithelial kidney cells of Macaca mulatta monkeys after gamma-irradiation in vivo and in vitro. The results of the experiments show that there are no differences in aberration yields produced in both types of somatic cells under gamma-irradiation in vivo and in vitro.

[Identification of monkey chromosomes by differential staining with Romanovsky--Giemza stain. III. Cercopithecus aethiops]. / Identifikatsiia khromosom ob'ezian metodom differentsial'nogo okrashivaniia rastvorom Romanovskogo--Gimza. Soobshchenie III. Cercopithecus aethiops.

The distribution of the G-bands in chromosomes of bone marrow cells of Cercopithecus aethiops was studied by means of differentail staining with the Romanovsky - Gimsa dye. All the homologous chromosome pairs were identified and morphometric parameters were detected. The comparison of C. aethiops karyotypes with those of Macacca mulatta has shown that they are different in numbers and in the character of banding pattern of the most chromosomes. Both species revealed 12 pairs of chromosomes similar in their morphology and parameters.

[Frequency of radiation-induced exchanges in simian marker chromosomes]. / Chastota indutsirovannykh radiatsiei obmenov v markernykh khromosomakh obeziian

The symmetric and asymmetric exchange frequencies of marked (nucleolus forming) chromosomes were studied in the lymphocytes and epithelial kidney cells irradiated by X-rays at Go, both in vivo and in vitro. Symmetric and asymmetric exchange frequencies were found to be equal. In both the types of Macaca mulatta cells, the exchange frequency in the long arm appeared to be higher than theoretically expected. The increased exchange in the long arm is thought to be due to a greater quantity of late replicating heterochromatin in it. The short arm of marked chromosome of epithelial kidney cells enters the exchange in accordance to its length in mitosis, but exchange number in the short arm chromosome in lymphocytes is lower than in epithelial cells. This difference is caused by different functioning of the nucleolus forming heterochromatin.

[Identification of simian chromosomes by a method of differential staining with Romanovskii--giemsa solution. II. Papio hamadryas]. / Identifikatsiia khromosom obez'ian metodom differentisial'nogo okrashivaniia rastvorom Romanovskogo--Gimza. Soobshchenie II. Papio hamadryas

By means of differential staining wiyh the Romanovsky-- Gimsa dye the distribution of G-bands was studied in chromosomes of bone marrow cells and of cultured peripheral blood lymphocytes in Papio hamadryas. All the homologous chromosome pairs were identified. It was shown, as compared Papio hamadryas karyotoypes with that of Macaca mulatta, that patterns of most chromosomes differentiation were similar. Certain differences between these species were observed in the character of differentiation of chromosomes 1 and 5 and also in parameters of chromosomes 2 and 21 (X).

[Identification of simian chromosomes by the differential staining method with Romanovskii-Giemza stain. II. Papio hamadryas]. / Identifikatsiia khromosom obez'ian metodom differentsial'nogo okrashivaniia rastvorom Romanovskogo-Gimza. Soobshchenie II. Papio hamadryas

By means of differential staining with the Romanovsky - Gimsa dye the distribution of G-bands was studied in chromosomes of bone marrow cells and of cultured peripheral blood lymphocytes in Papio hamadryas. All the homologous chromosome pairs were identified. It was shown, as compared Papio hamadryas karyotypes with that of Macaca mulatta, that patterns of most chromosomes differentiation were similar. Certain differences between these species were observed in the character of differentiation of chromosomes 1 and 5 and also in parameters of chromosomes 2 and 21 (X).