RESUMO
Screening of poorly growing colonies following EMS treatment of Caulobacter crescentus culture revealed several mutants with altered cell morphology. One of these (CB15-UW.5) grew as elongated, helically twisted cells which were more susceptible to autolysis than the wild type. The mutant cells were found to have higher transglycosylase activity, this being reflected by shorter chain length of their murein. A second difference between the murein of the mutant and the parent was lower trimer content in the former, although in both cases the degree of crosslinkage was similar.
Assuntos
Caulobacter crescentus/citologia , Caulobacter crescentus/genética , Mutação , Proteínas de Bactérias/análise , Soluções Tampão , Caulobacter crescentus/efeitos dos fármacos , Caulobacter crescentus/enzimologia , Caulobacter crescentus/crescimento & desenvolvimento , Membrana Celular/química , Quelantes/farmacologia , Ácido Edético/farmacologia , N-Acetil-Muramil-L-Alanina Amidase/metabolismo , Peptidoglicano/metabolismo , Fosfolipídeos/análise , Trometamina/farmacologiaRESUMO
Two F- mutants deficient in conjugation with F-donors have been characterized. They map at about 83 minut position, show resistance to T3 and T7 bacteriophages, and form mating aggregates in the liquid medium with lowered efficiency. Mutants have no detectable alterations in their outer membrane protein composition.
