Effects of temperature on metabolic rate during metamorphosis in the alfalfa leafcutting bee.

Spring conditions, especially in temperate regions, may fluctuate abruptly and drastically. Environmental variability can expose organisms to temperatures outside of their optimal thermal ranges. For ectotherms, sudden changes in temperature may cause short- and long-term physiological effects, including changes in respiration, morphology, and reproduction. Exposure to variable temperatures during active development, which is likely to occur for insects developing in spring, can cause detrimental effects. Using the alfalfa leafcutting bee, Megachile rotundata, we aimed to determine if oxygen consumption could be measured using a new system and to test the hypothesis that female and male M. rotundata have a thermal performance curve with a wide optimal range. Oxygen consumption of M. rotundata pupae was measured across a large range of temperatures (6-48°C) using an optical oxygen sensor in a closed respirometry system. Absolute and mass-specific metabolic rates were calculated and compared between bees that were extracted from their brood cells and those remaining in the brood cell to determine whether pupae could be accurately measured inside their brood cells. The metabolic response to temperature was non-linear, which is an assumption of a thermal performance curve; however, the predicted negative slope at higher temperatures was not observed. Despite sexual dimorphism in body mass, sex differences only occurred in mass-specific metabolic rates. Higher metabolic rates in males may be attributed to faster development times, which could explain why there were no differences in absolute metabolic rate measurements. Understanding the physiological and ecological effects of thermal environmental variability on M. rotundata will help to better predict their response to climate change.

Thermal history of alfalfa leafcutting bees affects nesting and diapause incidence.

Variable spring temperatures may expose developing insects to sublethal conditions, resulting in long-term consequences. The alfalfa leafcutting bee, Megachile rotundata, overwinters as a prepupa inside a brood cell, resuming development in spring. During these immobile stages of development, bees must tolerate unfavorable temperatures. In this study, we tested how exposure to low temperature stress during development affects subsequent reproduction and characteristics of the F1 generation. Developing male and female M. rotundata were exposed to either constant (6°C) or fluctuating (1 h day-1 at 20°C) low temperature stress for 1 week, during the pupal stage, to mimic a spring cold snap. Treated adults were marked and released into field cages, and reproductive output was compared with that of untreated control bees. Exposure to low temperatures during the pupal stage had mixed effects on reproduction and offspring characteristics. Females treated with fluctuating low temperatures were more likely to nest compared with control bees or those exposed to constant low temperature stress. Sublethal effects may have contributed to low nesting rates of bees exposed to constant low temperatures. Females from that group that were able to nest had fewer, larger offspring with high viability, suggesting a trade-off. Interestingly, offspring of bees exposed to fluctuating low temperatures were more likely to enter diapause, indicating that thermal history of parents, even during development, is an important factor in diapause determination.