Application of Absorption and Scattering Properties Obtained through Image Pre-Classification Method Using a Laser Backscattering Imaging System to Detect Kiwifruit Chilling Injury.

Kiwifruit chilling injury (CI) damage occurs after long-term exposure to low temperature. A non-destructive approach to detect CI injury was tested in the present study, using a laser backscattering image (LBI) technique calibrated with 56 liquid phantoms for providing absorption coefficient (µa) and reduced scattering coefficient (µs'). Calibration of LBI resulted in a true-positive (TP) classification of 91.5% and 65.6% of predicted µs' and µa, respectively. The optical properties of 'SunGold™'and 'Hayward' kiwifruit were analysed at 520 nm with a two-step protocol capturing pre-classification according to the LBI parameters used in the calibration and estimation with the Farrell equation. Severely injured kiwifruit showed white corky tissue and water soaking, reduced soluble solids content and firmness measured destructively. Non-destructive classification results for 'SunGold™' showed a high percentage of TP for severe CI of 92% and 75% using LBI parameters directly and predicted µa and µs' after pre-classification, respectively. The classification accuracy for severe CI 'Hayward' kiwifruit with LBI parameter was low (58%) and with µa and µs' decreased further (35%), which was assumed to be due to interference caused by the long trichomes on the fruit surface.

Measuring ethylene in postharvest biology research using the laser-based ETD-300 ethylene detector.

BACKGROUND: Ability to measure ethylene is an important aspect of postharvest management, as knowledge of endogenous ethylene production is used in assessing physiological status, while response of crops to exogenous ethylene informs efforts needed to control unwanted ripening. An ethylene monitoring device with a laser-based photoacoustic detector, ETD-300, was recently developed by Sensor Sense B.V., Nijmegen, The Netherlands. In terms of performance, the ETD-300 is superior to all other current ethylene measurement devices, with a sensitivity of 0.3 nL L-1, a response time of 5 s, and an ability to monitor ethylene in real time. Although the ETD-300 is relatively easy to operate, the performance and correctness of the data obtained depends on the choice of settings, which depends on the application. RESULTS: This article provides a description of different ways in which the ETD-300 can be used in postharvest research for monitoring ethylene production and ethylene presence in an environment. We provided guidelines on selecting the appropriate method (Continuous Flow, Stop and Flow, and Sample methods), and operational curves for deciding on suitable combination of free volume, flow rates, and period for the different measurement methods. CONCLUSIONS: Using these guidelines and operational curves, ETD-300 users can considerably reduce the measurement effort by limiting trial and error in establishing appropriate methodologies for their application. The guidelines also comment on accurate use of the ETD-300, as using the inappropriate settings could lead to erroneous measurements. Although these methodologies were developed primarily for postharvest application, they can be applied in other plant science research.