RESUMO
Purified native F1 antigen from Yersinia pestis was used to assess controlled-release vaccine delivery systems in poly(lactide-co-glycolide) (PLG) microparticles and liposomes. Antigen encapsulated in PLG microparticles induced high serum titres when injected i.p. in mice: mucosal IgA was also detected. Mice immunized with F1 in Alhydrogel or PLGs were protected against subcutaneous challenge with Y. pestis. F1 antigen surface-labelled onto liposome vesicles stimulated high serum titres in Balb/c mice and also induced a mucosal response: F1-labelled liposomes protected mice against challenge with up to 1 x 10(5) organisms. These findings indicate that a significant immune response is induced by immunizing with F1 formulated in PLGs and liposomes and that protection was achieved after only one dose.
Assuntos
Proteínas de Bactérias/imunologia , Vacinas Bacterianas/imunologia , Yersiniose/prevenção & controle , Yersinia pestis/imunologia , Administração Oral , Hidróxido de Alumínio , Animais , Anticorpos Antibacterianos/biossíntese , Proteínas de Bactérias/administração & dosagem , Composição de Medicamentos , Estudos de Avaliação como Assunto , Feminino , Imunoglobulina A/biossíntese , Injeções Intramusculares , Injeções Intraperitoneais , Lipossomos , Camundongos , Camundongos Endogâmicos BALB C , Poliglactina 910 , Yersiniose/imunologiaAssuntos
Proteínas de Bactérias/imunologia , Ativadores de Plasminogênio/imunologia , Yersinia pestis/imunologia , Anticorpos Antibacterianos/sangue , Anticorpos Monoclonais , Proteínas de Bactérias/química , Proteínas de Bactérias/isolamento & purificação , Cromatografia de Afinidade , Humanos , Técnicas In Vitro , Peso Molecular , Peste/diagnóstico , Peste/imunologia , Ativadores de Plasminogênio/química , Ativadores de Plasminogênio/isolamento & purificaçãoAssuntos
Antígenos de Bactérias/isolamento & purificação , Proteínas de Bactérias/isolamento & purificação , Yersinia pestis/imunologia , Animais , Biotecnologia , Humanos , Concentração de Íons de Hidrogênio , Camundongos , Peste/imunologia , Peste/prevenção & controle , Vacina contra a Peste/isolamento & purificaçãoRESUMO
We report on the specificity of a monoclonal antibody which reacts with autoclaved extracts of four species of enterococci but does not react to the same extent with similar extracts from two non-enterococcal group D streptococci. The monoclonal antibody also reacts specifically with purified lipoteichoic acid from Streptococcus faecalis but not significantly with purified lipoteichoic acid from the non-enterococcal species Streptococcus bovis and Streptococcus equinus. The specific antigen detected with this antibody could correlate with the definition of the enterococcus subgroup of the streptococci which would provide further evidence that this sub-group is taxonomically distinct from the other group D streptococci.
Assuntos
Anticorpos Monoclonais , Enterococcus/imunologia , Streptococcus/imunologia , Anticorpos Antibacterianos , Especificidade de Anticorpos , Antígenos de Bactérias , Enterococcus/classificação , Ensaio de Imunoadsorção Enzimática , Lipopolissacarídeos/imunologia , Especificidade da Espécie , Streptococcus/classificação , Ácidos Teicoicos/imunologiaRESUMO
A comparative study of methods to enumerate sulphite-reducing Clostridium spores and Group D faecal streptococci in oysters demonstrated that pour plate solid agar techniques gave higher counts than liquid broth most probable number procedures. Reinforced clostridial broth with supplements to detect sulphite reduction was compared with pour plates of egg yolk-free tryptose sulphite cycloserine agar incubated at 37 degrees C for 24 h. Azide dextrose broth was compared with pour plates using Slanetz and Bartley (SB) agar or KF-streptococcus agar at 37 degrees C. Most probable number procedures used for both groups of organisms gave excessive numbers of improbable tube combinations. For enumeration of Group D faecal streptococci, a pour plate technique using SB agar incubated at 37 degrees C for 48 h is recommended.