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1.
Nutrients ; 9(3)2017 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-28300770

RESUMO

Due to the functional properties and physiological effects often associated with chemically modified starches, significant interest lies in their development for incorporation in processed foods. This study investigated the effect of transglycosylated cornstarch (TGS) on blood glucose, insulin, and serum metabolome in the pre- and postprandial phase in growing pigs. Eight jugular vein-catheterized barrows were fed two diets containing 72% purified starch (waxy cornstarch (CON) or TGS). A meal tolerance test (MTT) was performed with serial blood sampling for glucose, insulin, lipids, and metabolome profiling. TGS-fed pigs had reduced postprandial insulin (p < 0.05) and glucose (p < 0.10) peaks compared to CON-fed pigs. The MTT showed increased (p < 0.05) serum urea with TGS-fed pigs compared to CON, indicative of increased protein catabolism. Metabolome profiling showed reduced (p < 0.05) amino acids such as alanine and glutamine with TGS, suggesting increased gluconeogenesis compared to CON, probably due to a reduction in available glucose. Of all metabolites affected by dietary treatment, alkyl-acyl-phosphatidylcholines and sphingomyelins were generally increased (p < 0.05) preprandially, whereas diacyl-phosphatidylcholines and lysophosphatidylcholines were decreased (p < 0.05) postprandially in TGS-fed pigs compared to CON. In conclusion, TGS led to changes in postprandial insulin and glucose metabolism, which may have caused the alterations in serum amino acid and phospholipid metabolome profiles.


Assuntos
Aminoácidos/química , Insulina/sangue , Metabolismo dos Lipídeos , Metaboloma , Amido/química , Aminoácidos/sangue , Animais , Glicemia/metabolismo , Dieta , Lisofosfatidilcolinas/metabolismo , Masculino , Modelos Animais , Fosfatidilcolinas/metabolismo , Período Pós-Prandial , Esfingomielinas/metabolismo , Suínos , Ureia/sangue
2.
J Vet Emerg Crit Care (San Antonio) ; 26(3): 373-83, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-27078857

RESUMO

OBJECTIVE: To evaluate the properties of RBC concentrate harvested after processing fresh whole blood units from healthy dogs with an automated cell salvage device. DESIGN: Prospective, in vitro, experimental study. SETTING: University teaching hospital. ANIMALS: Sixteen healthy, privately owned dogs of various breeds. INTERVENTIONS: Fresh canine whole blood collected in bags with citrate phosphate dextrose adenine solution was processed with an automated cell salvage device and analyzed in vitro. Laboratory values determined before (baseline, from a catheter sample) and after processing RBCs (procRBCs) included a complete blood count, selected blood chemistry analytes, erythrocyte osmotic resistance, whole blood viscosity, RBC aggregation, and RBC deformability. MEASUREMENTS AND MAIN RESULTS: Total recovery of RBCs was 80% ± 12%. Hematocrit of the procRBCs yielded by the device was 77% ± 3.7% (mean ± standard deviation). Gross morphology of the RBCs remained unchanged. The mean corpuscular volume, erythrocyte osmotic resistance, RBC deformability, RBC aggregation, and the activity of lactate dehydrogenase showed minor but statistically significant changes from baseline. No differences in the concentrations of free hemoglobin were observed. Whole blood viscosity was less in the procRBCs. Seventy-seven percent (mean) of the platelets were washed out, while a mean of 57% of the leukocytes remained in the procRBCs. CONCLUSIONS: Although processing canine blood with this automated cell salvage device leads to slight changes in some properties of RBCs, most of these changes are comparable to changes seen in human blood after processing. Present data indicate that the use of this cell salvage device does not induce changes in canine RBC concentrate that would preclude its use for transfusion.


Assuntos
Remoção de Componentes Sanguíneos/instrumentação , Preservação de Sangue/veterinária , Doenças do Cão/terapia , Transfusão de Eritrócitos/veterinária , Eritrócitos/citologia , Animais , Automação , Remoção de Componentes Sanguíneos/veterinária , Cães , Estudos Prospectivos
3.
PLoS One ; 10(6): e0130553, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26076487

RESUMO

Developing host digestion-resistant starches to promote human health is of great research interest. Chemically modified starches (CMS) are widely used in processed foods and although the modification of the starch molecule allows specific reduction in digestibility, the metabolic effects of CMS have been less well described. This short-term study evaluated the impact of enzymatically modified starch (EMS) on fasting and postprandial profiles of blood glucose, insulin and lipids, and serum metabolome in growing pigs. Eight jugular-vein catheterized pigs (initial body weight, 37.4 kg; 4 months of age) were fed 2 diets containing 72% purified starch (EMS or waxy corn starch (control)) in a cross-over design for 7 days. On day 8, an 8-hour meal tolerance test (MTT) was performed with serial blood samplings. Besides biochemical analysis, serum was analysed for 201 metabolites through targeted mass spectrometry-based metabolomic approaches. Pigs fed the EMS diet showed increased (P<0.05) immediate serum insulin and plasma glucose response compared to pigs fed the control diet; however, area-under-the-curves for insulin and glucose were not different among diets. Results from MTT indicated reduced postprandial serum triglycerides with EMS versus control diet (P<0.05). Likewise, serum metabolome profiling identified characteristic changes in glycerophospholipid, lysophospholipids, sphingomyelins and amino acid metabolome profiles with EMS diet compared to control diet. Results showed rapid adaptations of blood metabolites to dietary starch shifts within 7 days. In conclusion, EMS ingestion showed potential to attenuate postprandial raise in serum lipids and suggested constant alteration in the synthesis or breakdown of sphingolipids and phospholipids which might be a health benefit of EMS consumption. Because serum insulin was not lowered, more research is warranted to reveal possible underlying mechanisms behind the observed changes in the profile of serum lipid metabolome in response to EMS consumption.


Assuntos
Carboidratos da Dieta/farmacologia , Metaboloma/efeitos dos fármacos , Período Pós-Prandial/fisiologia , Amido/metabolismo , Triglicerídeos/sangue , Ração Animal , Animais , Glicemia/análise , Dieta , Digestão/fisiologia , Insulina/sangue , Metabolismo dos Lipídeos , Masculino , Amido/química , Amido/farmacologia , Suínos
5.
Anesth Analg ; 110(1): 181-7, 2010 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-19910629

RESUMO

BACKGROUND: We investigated the effects of 4 h of posttraumatic hyperventilation on neurocognitive performance, motor function, and coordination as well as lesion volume in rats subjected to focal traumatic brain injury. METHODS: After a 14-day training period with various neurocognitive tests including hole-board, beam walk, and beam balance, 21 male Sprague-Dawley rats (369 +/- 15 g) were anesthetized with halothane, tracheally intubated, their lungs mechanically ventilated, and subjected to controlled cortical impact (1.75 mm depth, diameter 5 mm, 4 m/s). They were then randomized to either normoventilation (n = 10; PaCO(2) = 38-42 mm Hg) or hyperventilation (n = 11; PaCO(2) = 28-32 mm Hg) and ventilated for 4 h, respectively. Posttraumatic performance in the behavioral and motor tests was evaluated for 20 days. Rats were then decapitated under deep anesthesia, and their brains frozen and sliced to evaluate lesion volume. RESULTS: Hyperventilated animals performed significantly worse in explicit memory tests compared with normoventilated rats over time. Both groups showed deficits in advanced motor function and coordination (evaluated by beam walk and beam balance) initially, with a significantly worse performance of hyperventilated compared with normoventilated animals. However, there was no difference between groups by the end of the study. On Day 20 after injury, lesion volume was significantly larger with hyperventilated (69.7 +/- 13.0 mm(3)) compared with normoventilated animals (48.3 +/- 15.6 mm(3)). CONCLUSIONS: Although hyperventilation enhanced histologic damage, there was no long-term adverse neurocognitive effect from 4 h of posttraumatic hyperventilation (PaCO(2) = 28-32 mm Hg) in rats.


Assuntos
Lesões Encefálicas/patologia , Lesões Encefálicas/psicologia , Córtex Cerebral/lesões , Cognição/fisiologia , Hiperventilação/psicologia , Desempenho Psicomotor/fisiologia , Anestesia , Animais , Ansiedade/psicologia , Gasometria , Córtex Cerebral/patologia , Comportamento Exploratório/fisiologia , Hemodinâmica/fisiologia , Masculino , Aprendizagem em Labirinto , Memória/fisiologia , Ratos , Ratos Sprague-Dawley
6.
Vet Anaesth Analg ; 35(3): 249-55, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18282254

RESUMO

OBJECTIVE: To characterize the pharmacokinetics of fentanyl and the tolerance of foals to the drug following a single application of a commercially available transdermal system (TS). STUDY DESIGN: Prospective experimental study. ANIMALS: Six (two male, four female) foals aged 4-8 days, weighing 56-74 kg. METHODS: After placement of a jugular sampling catheter, one fentanyl TS (FTS) containing 10.2 mg fentanyl, released at 100 microg hour(-1), was applied for 72 hours. Blood samples were withdrawn over the course of 90 hours for fentanyl plasma analysis. Before and after the study, weight, complete blood count and blood chemistry values were obtained. During the study, tolerance and safety were monitored by physical examination and assessment of behavior. RESULTS: Fentanyl was detected as early as 20 minutes after FTS placement. Peak plasma concentrations were variable (0.1-28.7 ng mL(-1)), were reached after 14.3 +/- 7.6 hours (mean +/- SD), and returned to baseline concentrations 12 hours after FTS removal. All foals satisfactorily tolerated the FTS application and no significant adverse effects were observed. Rectal temperature increased above 38.5 degrees C (max. 39.0 degrees C) in all foals, although this did not correlate with fentanyl plasma concentrations. Results of hematological and biochemical analyses were within reference ranges. CONCLUSION AND CLINICAL RELEVANCE: Our data show that 100 microg hour(-1) fentanyl administered by an FTS results in time-related but variable plasma concentrations in foals. The FTS was easy to apply and was well tolerated.


Assuntos
Analgésicos Opioides/sangue , Analgésicos Opioides/farmacocinética , Fentanila/sangue , Fentanila/farmacocinética , Cavalos/sangue , Administração Cutânea , Analgésicos Opioides/administração & dosagem , Analgésicos Opioides/efeitos adversos , Animais , Feminino , Fentanila/administração & dosagem , Fentanila/efeitos adversos , Masculino , Absorção Cutânea
7.
Anesth Analg ; 104(4): 898-903, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17377103

RESUMO

BACKGROUND: The effect of sevoflurane on the neuroregenerative potential after neuronal injury is unclear. We investigated the effect of low and high concentrations of sevoflurane on endogenous neurogenesis after cerebral ischemia. METHODS: Anesthetized and ventilated rats were randomized to four different treatment groups. Groups 1 and 2: 1.4% sevoflurane; Groups 3 and 4: 2.8% sevoflurane. In Groups 1 and 3, no cerebral ischemia was induced (sham-operated). In Groups 2 and 4, 10 min of forebrain ischemia was induced by bilateral carotid artery occlusion plus hemorrhagic hypotension. Physiological variables were maintained constant. Bromodeoxyuridine was given as a marker of neurogenesis. After 28 days brains were perfused. Histopathological damage of the hippocampus was evaluated in hematoxylin and eosin (HE) stained sections using the HE-index (0 = no damage; 1 = 1%-10% damage; 2 = 11%-50% damage; 3 = 51%-100% damage). Immunohistochemistry was used to detect bromodeoxyuridine-positive neurons. Eight untreated rats were investigated as naive controls (Group 5). RESULTS: In neither sham-operated group was histopathological damage or change in neurogenesis observed compared to naive controls. In rats anesthetized with 1.4% sevoflurane, cerebral ischemia caused mild neuronal damage (HE-index of 0.64 +/- 0.84) and increased neurogenesis by 60% when compared with respective sham-operated animals; with 2.8% sevoflurane, the HE-index was 1.22 +/- 1.14, and the number of newly generated neurons increased by 230% when compared with respective sham-operated animals. CONCLUSION: The present data suggest that high concentrations of sevoflurane stimulate neurogenesis in the dentate gyrus after cerebral ischemia.


Assuntos
Anestésicos Inalatórios/farmacologia , Isquemia Encefálica/fisiopatologia , Giro Denteado/efeitos dos fármacos , Éteres Metílicos/farmacologia , Regeneração Nervosa/efeitos dos fármacos , Prosencéfalo/fisiopatologia , Animais , Isquemia Encefálica/patologia , Proliferação de Células/efeitos dos fármacos , Giro Denteado/patologia , Giro Denteado/fisiopatologia , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Hipocampo/efeitos dos fármacos , Hipocampo/fisiopatologia , Masculino , Neurônios/efeitos dos fármacos , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Sevoflurano
8.
Anesth Analg ; 103(6): 1527-33, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17122234

RESUMO

BACKGROUND: Propofol is commonly used to sedate patients after traumatic brain injury. However, the dose-dependent neuroprotective effects of propofol after head trauma are unknown. We compared histopathological damage after 6 h of electroencephalogram-targeted high- and low-dose propofol infusion in rats subjected to controlled cortical impact (CCI). METHODS: Animals were randomly assigned to CCI/propofol with electroencephalogram burst-suppression-ratio 1%-5% (CCI/lowprop), CCI/propofol with burst-suppression-ratio 30%-40% (CCI/highprop), control group CCI/1.0 vol % halothane (CCI/halo), or sham group with halothane anesthesia (SHAM/halo). Brain slices were stained with kresyl violet (KV) and hematoxylin/eosin (HE) to evaluate lesion volume, number of eosinophilic cells, and activation of caspase-3 in the hippocampus. RESULTS: Lesion volume (mm3) and number of eosinophilic cells in the hippocampus did not differ significantly [lesion volumes: CCI/lowprop 31.55 +/- 14.66 (KV) and 53.77 +/- 8.62 (HE); CCI/highprop 33.81 +/- 10.57 (KV) and 52.30 +/- 11.55 (HE); CCI/halo 36.42 +/- 17.06 (KV) and 57.95 +/- 8.49 (HE)]. Activation of caspase-3 occurred in the ipsilateral hippocampus in all CCI-groups. CONCLUSION: Despite different levels of cortical neuronal function, there were no relevant differences in the short-term histopathological damage. These results challenge the view that the neuroprotective effect of propofol relates to the suppression of cerebral metabolic demand.


Assuntos
Lesões Encefálicas/patologia , Encéfalo/patologia , Eletroencefalografia/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Propofol/farmacologia , Animais , Lesões Encefálicas/fisiopatologia , Caspase 3/metabolismo , Relação Dose-Resposta a Droga , Eosinófilos/patologia , Halotano/farmacologia , Infusões Intravenosas , Contagem de Leucócitos , Masculino , Ratos , Ratos Sprague-Dawley
9.
Anesth Analg ; 103(1): 173-9, table of contents, 2006 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16790648

RESUMO

We investigated the long-term effects of sevoflurane on histopathologic injury and key proteins of apoptosis in a rat hemispheric ischemia/reperfusion model. Sixty-four male Sprague-Dawley rats were randomly assigned to Group 1 (fentanyl and N2O/O2; control) and Group 2 (2.0 vol% sevoflurane and O2/air). Ischemia (45 min) was produced by unilateral common carotid artery occlusion plus hemorrhagic hypotension (mean arterial blood pressure 40 mm Hg). Animals were killed after 1, 3, 7, and 28 days. In hematoxylin and eosin-stained brain sections eosinophilic hippocampal neurons were counted. Activated caspase-3 and the apoptosis-regulating proteins Bax, Bcl-2, Mdm-2, and p53 were analyzed by immunostaining. No eosinophilic neurons were detected in sevoflurane-anesthetized rats over time, whereas 9%-38% of the hippocampal neurons were eosinophilic (days 1-28) in control animals. On days 1 and 3, the concentration of Bax was 140%-200% larger in fentanyl/N2O-anesthetized animals compared with sevoflurane. Bcl-2 was 100% less in control animals during the first 3 days. Activated caspase-3 was detected in neurons of both groups (0.75%-2.2%). These data support a sustained neuroprotective potency of sevoflurane related to reduced eosinophilic injury after cerebral ischemia/reperfusion.


Assuntos
Anestésicos Inalatórios/farmacologia , Proteínas Reguladoras de Apoptose/metabolismo , Isquemia Encefálica/metabolismo , Isquemia Encefálica/patologia , Hipocampo/patologia , Éteres Metílicos/farmacologia , Neurônios/patologia , Fármacos Neuroprotetores/farmacologia , Traumatismo por Reperfusão/metabolismo , Animais , Velocidade do Fluxo Sanguíneo , Isquemia Encefálica/fisiopatologia , Caspase 3 , Caspases/metabolismo , Contagem de Células , Circulação Cerebrovascular , Ativação Enzimática , Hipocampo/metabolismo , Imuno-Histoquímica , Masculino , Neurônios/metabolismo , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/patologia , Traumatismo por Reperfusão/fisiopatologia , Traumatismo por Reperfusão/prevenção & controle , Sevoflurano
10.
Anesthesiology ; 101(4): 912-7, 2004 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-15448524

RESUMO

BACKGROUND: Propofol reduces neuronal damage from cerebral ischemia when investigated for less than 8 postischemic days. This study investigates the long-term effects of propofol on neuronal damage and apoptosis-related proteins after cerebral ischemia and reperfusion. METHODS: Male Sprague-Dawley rats were randomly assigned as follows: group 1 (n = 32, control): fentanyl and nitrous oxide-oxygen; group 2 (n = 32, propofol): propofol and oxygen-air. Ischemia (45 min) was induced by carotid artery occlusion and hemorrhagic hypotension. Pericranial temperature and arterial blood gases were maintained constant. After 1, 3, 7, and 28 postischemic days, brains were removed, frozen, and sliced. Hippocampal eosinophilic cells were counted. The amount of apoptosis-related proteins Bax, p53, Bcl-2, and Mdm-2 and neurons positive for activated caspase-3 were analyzed. RESULTS: In propofol-anesthetized rats, no eosinophilic neurons were detected, whereas in control animals, 16-54% of hippocampal neurons were eosinophilic (days 1-28). In control animals, the concentration of Bax was 70-200% higher after cerebral ischemia compared with that in animals receiving propofol over time. Bcl-2 was 50% lower in control animals compared with propofol-anesthetized rats during the first 3 days. In both groups, a maximal 3% of the hippocampal neurons were positive for activated caspase-3. CONCLUSIONS: These data show sustained neuroprotection with propofol. This relates to reduced eosinophilic and apoptotic injury. Activated caspase-3-dependent apoptotic pathways were not affected by propofol. This suggests the presence of activated caspase-3-independent apoptotic pathways.


Assuntos
Apoptose/efeitos dos fármacos , Isquemia Encefálica/patologia , Neurônios/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Propofol/farmacologia , Animais , Caspase 3 , Caspases/fisiologia , Masculino , Neurônios/patologia , Proteínas Proto-Oncogênicas c-bcl-2/fisiologia , Ratos , Ratos Sprague-Dawley , Reperfusão
11.
J Neurosurg Anesthesiol ; 15(3): 200-8, 2003 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12826967

RESUMO

This study investigated the effects of hypothermia on apoptosis-regulating proteins in a rat model of incomplete cerebral ischemia. Twenty-seven fasted male Sprague-Dawley rats (300-420 g) were anesthetized, intubated, and mechanically ventilated with 2.0% isoflurane and N(2)O/O(2) (FiO(2) = 0.33). Catheters were inserted and cerebral blood flow velocity was measured using bilateral laser Doppler flowmetry. At the end of preparation, the administration of isoflurane was replaced by fentanyl (25 microg. kg(-1). h(-1)). Animals were randomly assigned to one of the following groups: group 1 (n = 9, normothermia), normothermia (37.5 degrees C) during ischemia; group 2 (n = 9, hypothermia), 34 degrees C pericranial temperature during ischemia; and group 3 (n = 9, sham-operated animals), normothermia, no cerebral ischemia. Ischemia (30 minutes) was produced by unilateral common carotid artery occlusion plus hemorrhagic hypotension (mean arterial blood pressure 30-35 mm Hg). Arterial blood gas tensions and pH were maintained constant. Four hours after 30 minutes of incomplete cerebral ischemia, the brains were removed for determination of the expression of the apoptosis-regulating proteins Bax, Bcl-2, p53, and Mdm-2 using immunofluorescence and Western blot analysis. Four hours after cerebral ischemia there was a significant increase in the expression of the pro-apoptotic protein Bax in normothermic animals compared with hypothermic (85-260%) and sham-operated animals (60-190%). The proteins Bcl-2, p53, and Mdm-2 showed no statistically significant differences between the groups or between the hemispheres. In conclusion, hypothermia during ischemia decreased Bax protein expression that is associated with programed cell death. This suggests that neuroprotection seen with hypothermia may be related to a reduction of pro-apoptotic events.


Assuntos
Apoptose/fisiologia , Isquemia Encefálica/genética , Regulação da Expressão Gênica/fisiologia , Hipotermia Induzida , Proteínas Proto-Oncogênicas c-bcl-2 , Proteínas Proto-Oncogênicas/biossíntese , Traumatismo por Reperfusão/genética , Animais , Western Blotting , Isquemia Encefálica/patologia , Imunofluorescência , Hemodinâmica/fisiologia , Fluxometria por Laser-Doppler , Masculino , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/patologia , Fatores de Tempo , Proteína X Associada a bcl-2
12.
Anesth Analg ; 96(2): 524-31, table of contents, 2003 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-12538207

RESUMO

In this study, we investigated whether the neuroprotection previously seen with dexmedetomidine or S(+)-ketamine involves regulation of proapoptotic (Bax and p53) and antiapoptotic (Bcl-2 and Mdm-2) proteins. Rats were anesthetized with isoflurane. After surgical preparation of isoflurane was discontinued, animals were randomly assigned to receive fentanyl and nitrous oxide (N(2)O)/oxygen plus 100 microg/kg of dexmedetomidine intraperitoneally 30 min before ischemia (n = 8), 1 mg x kg(-1) x min(-1) of S(+)-ketamine and oxygen/air (n = 8), or fentanyl and N(2)O/oxygen (n = 8; control group). In all three treatment groups, incomplete cerebral ischemia (30 min) was induced by unilateral carotid artery occlusion and hemorrhagic hypotension to a mean arterial blood pressure of 30-35 mm Hg. Four hours after the start of reperfusion, the brains were removed, and the expression of apoptosis-regulating proteins was determined by using immunofluorescence and Western blot analysis. The results were compared with sham-operated animals (n = 8). After cerebral ischemia/reperfusion, the relative protein concentration of Bax was increased by 110% in control animals compared with the dexmedetomidine- and S(+)-ketamine-treated rats and by 140% compared with the sham-operated animals. In animals treated with dexmedetomidine, the expression of Bcl-2 and Mdm-2 was larger compared with control (68% and 210%, respectively) or sham-operated (110% and 180%, respectively) animals. Therefore, it is possible that the neuroprotective properties of dexmedetomidine and S(+)-ketamine seen in previous studies involve ultra-early modulation of the balance between pro- and antiapoptotic proteins.


Assuntos
Agonistas de Receptores Adrenérgicos alfa 2 , Agonistas alfa-Adrenérgicos/farmacologia , Apoptose/efeitos dos fármacos , Apoptose/genética , Isquemia Encefálica/genética , Dexmedetomidina/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Ketamina/farmacologia , Receptores de N-Metil-D-Aspartato/antagonistas & inibidores , Traumatismo por Reperfusão/genética , Animais , Gasometria , Western Blotting , Química Encefálica/efeitos dos fármacos , Isquemia Encefálica/patologia , Circulação Cerebrovascular/efeitos dos fármacos , Imunofluorescência , Hemodinâmica/efeitos dos fármacos , Masculino , Inibidores de Proteases/farmacologia , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/patologia
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