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ABSTRACT Nanobiotechnology is one of the most promising areas in modern nanoscience and technology. Metallic nanoparticles have found uses in many applications in different fields, such as catalysis, photonics, electronics, medicine and agriculture. Synthesized nanoparticles through chemical and physical methods are expensive and have low biocompatibility. In the present study, silver nanoparticles have been synthesized from Megaphrynium macrostachyum (Benth. & Hook. f.) Milne-Redh., Marantaceae, leaf extract. Megaphrynium macrostachyum is a plant with large leaves found in the rainforest of West and Central Africa. Synthetic optimizations following factors such as incubation time, temperature, pH, extract and silver ion concentration during silver formation are discussed. UV–visible spectra gave surface plasmon resonance for synthesized silver nanoparticles based Megaphrynium macrostachyum peaks at 400–450 nm. X-ray diffraction revealed the average size of pure crystallites composed from Ag and AgCl.
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Background:Health care acquired infections are a major cause of morbidity and mortality in hospitals. There is very little documentation of this important problem in our resource limited setting. The aim of our study was to identify bacterial reservoirs and microorganisms with the potential of nosocomial infections in our health care environment Methods:Bacteriological samples from the air; surfaces; equipment; personnel and patients from six units of the Douala General Hospital; Cameroon were collected for culture according to standardised collection; culture and germs identification techniques. of all collected samples were contaminated with pathogenic bacteria. These included 83.3 of air samples and 100; 54.1and 48.7of specimens respectively. The same bacteria found on the hands of nursing personnel were same as on both surfaces and equipment. The intensive care and neonatal units were the most contaminated with 100 positive cultures. The sterilization unit was bacteria free
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Camarões , Progressão da Doença , Reservatórios de Doenças , Meio Ambiente e Saúde Pública , Hospitais GeraisRESUMO
Pseudomonas aeruginosa is a germ of hospitalism responsible for nosocomial infections; it is naturally resistant to many antibiotics and has a high susceptibility to the acquisition of acquiring new resistance. The observation of strains highly resistant to antibiotics; has led us to look for possible alternative therapeutics. This study was a descriptive and cross-sectional one; conducted from October 2010 to March 2011. All patients hospitalized for at least 48 hours and showing sign of infection were included after obtaining their consent. Forty nine of 150 samples were positive to the cultivation of Pseudomonas aeruginosa showing a prevalence of 32.66%. For the antibiotic susceptibility; we obtain amikacin 57.14%; netilmicin 59.20%; ceftazidime 52.60%; imipenem 33%; colistin 97.95%; and ciprofloxacin 51%. Seven strains were resistant to all antibiotics tested other than colistin. One strain was resistant to colistin. Colistin retains high sensitivity to Pseudomonas aeruginosa. However; there are some strains multiresistant to antibiotics
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Camarões , Colistina , Resistência a Medicamentos , Pseudomonas aeruginosaRESUMO
We report one case of rare facial entomophtoromycosis which occured in a young male patient of 16 years of age, in Cameroon. The clinical presentation was a central mass of the face. We show the interest of the diagnosis of this rare fungal infection through biopsy and its medical treatment.