Effect of different application strategies of universal adhesive used for immediate and delayed dentin sealing on the microtensile bond strength of self-adhesive resin cement to dentin with and without aging.

Background: Bond strength of indirect restorations is a very important issue that should be given special attention. Immediate dentin sealing (IDS) technique has been suggested in recent years. The aim of the present study was to investigate the effect of different strategies of universal adhesive application for immediate and delayed dentin sealing (DDS) on the microtensile bond strength (µTBS) of self-adhesive resin cement with and without aging. Material and Methods: In this experimental study, 24 healthy human third molars were selected. After exposing the occlusal dentin, the teeth were randomly divided into two groups of 12 based on the All-Bond Universal adhesive application strategy (etch-and-rinse or self-etch). Each group was further subdivided into two (n=6) based on IDS or DDS technique. Then composite blocks were cemented on the occlusal surface with self-adhesive resin cement. After cutting the samples into 1 mm2 cross-sections, half of the samples of each subgroup were subjected to µTBS test after one week, and the other half were tested under µTBS after 10,000 thermal cycles. Data were analyzed using three-way ANOVA (P<0.05). Results: µTBS was significantly affected by all three factors of bond strategy, sealing technique and aging. There was also a significant interaction between the three factors. Conclusions: Immediate dentin sealing improved µTBS. The etch-and-rinse strategy resulted in higher µTBS, while aging led to a decrease in µTBS. Key words:Adhesives, dental bonding, dentin, universal, sealing.

Effect of at-home and in-office bleaching on microleakage of class V composite resin restorations using different types of universal adhesives: An in vitro study.

Background: When bleaching agents contact dental structures, they act on restorative materials and adhesive interfaces. This study investigated the effect of "at-home" and "in-office" bleaching on the microleakage of composite resin restorations performed with different universal adhesives in self-etch and etch-and-rinse modes. Methods: Class V cavities were prepared in 132 premolars. The samples were divided into four groups (n=33). All Bond Universal adhesive was used in the first and second groups, and G-Premio Bond adhesive was used in the third and fourth groups. The total-etch mode was used in the first and third groups, and the self-etch mode was used in the second and fourth groups. The samples were divided into three subgroups (n=11). In the first subgroup, home bleaching was used, and in the second subgroup, office bleaching was used. In the third subgroup, bleaching was not performed. The specimens were examined under a stereomicroscope for microleakage. Ordinal regression analysis was applied (P<0.05). Results: The adhesive type, application method, and margin type significantly affected microleakage (P<0.05). The amount of microleakage in All Bond Universal adhesive was significantly higher than in G-Premio Bond adhesive. The chance of microleakage in the self-etch mode was almost twice as high as in the etch-and-rinse mode. The bleaching method did not significantly affect microleakage (P>0.05). Conclusion: Based on the results of the microleakage test, bleaching after composite resin restorations did not significantly affect the microleakage of Class V restorations.

Effect of intraradicular reinforcement strategies on the fracture strength of endodontically treated anterior teeth with overflared canals.

BACKGROUND: This study is done to evaluate the effect of different reinforcement techniques of overflared root canals with fiber posts on the fracture resistance of endodontically treated anterior teeth. It also introduces a new technique for simultaneous reinforcement and cementation of fiber posts using dual-cured core build-up composite resin. MATERIAL AND METHODS: The crowns of 48 single-rooted maxillary anterior teeth were cut and randomly divided into 4 groups (n=12) based on root reinforcement techniques after root canal treatment and post space preparation: Group 1: self-adhesive resin cement (SARC), Group2: dual-cure composite resin (DCC), Group3: Composite resin reinforcement (CRR), Group 4: Direct anatomical post (DAP). Then a core was made for the roots. The periodontal ligament was simulated using a thin layer of PVC impression material. A universal testing machine applied a compressive force to the lingual surface at an angle of 135 ° and 1 mm/min speed. Data were analyzed using One-Way ANOVA and a post-hoc Tukey test. Fracture patterns were analyzed using the Chi-square test (p<0.05). RESULTS: The difference between the control and DAP group was statistically significant (p=0.01), but there were no significant differences between other groups (p>0.05). Regarding fracture patterns, there were no significant differences between groups. CONCLUSIONS: All reinforcement techniques increased the fracture strength compared to the control group. However, DAP was the only group that had a statistically significant difference. CRR and DCC presented intermediate values without significant differences. Key words:Fiber post, endodontically treated teeth, root canals, reinforcement, fracture strength.

Comparison of the effect of preheating on the flexural strength of giomer and nanohybrid composite resin.

Background. Considering the increased use of preheating and novel resin-based materials to restore teeth, the present study investigated the impact of preheating on the flexural strength of a giomer and compared it with a nanohybrid composite resin. Methods. Two restorative materials (Beautifil II giomer and Alpha III nanohybrid composite resin) were used. Thirty rod-shaped samples (adding up to 60 samples) were prepared from the materials above and divided into two subgroups: with and without preheating (n=15). Before sample preparation, the giomer and nanohybrid composite resin tubes were preheated at 68ºC for 15 minutes in the preheating subgroups. In the subgroups without preheating, the tubes were kept in a room at 25ºC. Then the flexural strength was compared between the two groups with two-way ANOVA at a significance level of P<0.05. Results. The results showed significantly higher flexural strength in the preheated subgroups than in the non-preheated subgroups (P<0.001). In addition, the mean flexural strength values were significantly higher in the giomer groups than in the nanohybrid composite resin groups (P<0.001). Conclusion. Preheating increased the studied materials' flexural strengths significantly. The flexural strength of the giomer restorative material was higher than that of the nanohybrid composite resin, irrespective of preheating.

The effects of different surface contaminators on the shear bond strength of a universal adhesive system to dentin: an experimental study.

Background. Contamination of dentin surface is one of the common problems in restorative dentistry. The aim was to investigate the effects of different surface contaminators on the dentin shear bond strength (SBS) of universal adhesive system (UAS) applied in etch-and-rinse (ER) and self-etch (SE) strategies. Methods. One hundred forty-four maxillary anterior sound human teeth were divided into six groups based on the types of surface contaminators: no surface contaminator (control) and experimental groups contaminated with blood, saliva, aluminium chloride (ALC), ferric sulphate (FS), and caries disclosing agent (CDA). Then, each group was further subdivided into two, based on the application strategy of UAS (ER and SE). After applying the adhesive according to the manufacturer's instructions, and bonding cylindrical composite samples, the SBS was measured. The data were analysed using two-way ANOVA, Tukey's HSD test and t test (P < 0.05). Results. The SBS in all contaminated groups, except for CDA, was significantly lower in both ER and SE strategies compared to control group (P < 0.05). A comparison between the application strategies revealed that ER and SE were only significantly different in the FS contaminated group (P < 0.05). Conclusion. All tested contaminators, except CDA, significantly decreased SBS of UAS in both ER and SE strategies.

The effect of pre-heating on monomer elution from bulk-fill resin composites.

BACKGROUND: The present study was aimed to evaluate the effect of pre-heating of bulk -fill resin composites on monomer elution from them. MATERIAL AND METHODS: Three different types of resin composites were used including Tetric N-Ceram Bulk Fill, X-tra Fill and X-tra Base. 10 cylindrical samples were prepared from each resin composites. Before light curing, 5 samples were pre-heated until reaching 68˚C, then 5 other samples were polymerized at room temperature. After 24 hours, release of UDMA, TEGDMA and BIS-GMA monomers were measured by High-Performance Liquid Chromatography analysis. Data analysis was performed by two-way ANOVA test, Games-Howell and Sidak post hoc tests. RESULTS: Pre-heating did not have any statistically significant effect on the mean values of UDMA, TEGDMA and Bis-GMA elution (p>0.05). The greatest amount of released Bis-GMA and UDMA was obtained from Tetric N-Ceram Bulk-fill composite. The greatest amount of released TEGDMA was obtained from X-tra Fill composite. X-tra Base composite showed the lowest amount of monomer release (P<0.001). CONCLUSIONS: Pre-heating did not have any effect on monomer release from bulk-fill resin composites. Moreover, the amount and the type of monomers released from various bulk-fill resin composites were not similar. Key words:Bulk fill composite resin, elution, HPLC, residual monomer, temperature.

Effect of preheating on the cytotoxicity of bulk-fill composite resins.

Background. Due to the effect of pre-heating on the degree of conversion of composite resins and the possible effect on cytotoxicity, the effect of pre-heating of bulk-fill composite resins was investigated on cytotoxicity in this study. Methods. In this study, three different types of composite resin were used, including Tetric N-Ceram Bulk-Fil, Xtrafil, and Xtrabase. From each composite resin, 10 cylindrical samples (5 mm in diameter and 4 mm in height) were prepared, with five samples preheated to 68°C, and the other five samples polymerized at room temperature (25°C). Twenty-four hours after polymerization, cytotoxicity was assessed by MTT assay on human fibroblasts. Statistical analysis of data was carried out with two-way ANOVA and Sidak Post-Hoc. The significance level of the test was determined at 0.05. Results. There was no statistically significant difference between the mean percentage of cytotoxicity in terms of pre-heating (P>0.05), but the cytotoxicity of the studied composite resins was significantly different (P<0.001). The cytotoxicity of Tetric N-Ceram Bulk-fil composite resin was higher than that of the two other composite resins. Conclusion. Pre-heating of bulk-fill composite resin did not affect their cytotoxicity. In addition, the cytotoxicity of different bulk-fill composite resins was not the same.

Surface Roughness of Different Composite Resins after Application of 15% Carbamide Peroxide and Brushing with Toothpaste: An In-Vitro Study.

OBJECTIVES: This study aimed to evaluate the effect of toothbrushing after application of 15% carbamide peroxide (CP) on the surface roughness of three types of composite resins. MATERIALS AND METHODS: Twenty samples, measuring 4 mm in height and diameter, were fabricated of three composite resins namely microfilled (MF) Heliomolar HB, nanohybrid (NH) IPS Empress Direct and microhybrid (MH) Tetric Ceram HB. After polishing, the initial surface roughness was measured with a profilometer. The tray technique was used to apply 15% CP gel for 6 hours. Then, cleaning was carried out with an Oral-B electric toothbrush for 3 minutes in a tank containing a freshly mixed toothpaste. These procedures were repeated for 21 days. Then, the surface roughness was measured again and compared with the initial values. A mixed-design ANOVA model was used for the analysis of data (P<0.05). RESULTS: The baseline roughness was significantly lower in MF compared to the NH and MH composites (P<0.001). Roughness increased in all study groups during the intervention period; however, this increase was not significant in the MH group (P=0.17). Furthermore, the increase in roughness in MF was smaller than that in NH (P<0.001) and MH (P=0.02) groups. CONCLUSION: The effect of intervention was more pronounced on NH and MH groups. Surface roughness changes were minor in MF composite resin.

Effect of bleaching protocols on surface roughness and biofilm formation on silorane-based composite resin.

BACKGROUND: Knowledge about the effect of bleaching on behavior of composite resins is important to find a suitable composite resin for restoration of teeth undergoing bleaching. This study aimed to assess the effect of different bleaching protocols on surface roughness and biofilm formation on a silorane-based composite resin. MATERIALS AND METHODS: In this in vitro experimental study, 60 silorane-based composite resin samples measuring 3 mm in thickness and 6 mm in diameter were fabricated and polished. They were then randomly divided into four groups (n = 15). In Group 1, samples were stored in distilled water as control. Samples in Groups 2, 3, and 4 were subjected to bleaching with 15% carbamide peroxide, 35% hydrogen peroxide, and 35% hydrogen peroxide activated by light, respectively. Surface roughness was measured using a profilometer. Streptococcus mutans cultured in brain-heart infusion broth was used for the assessment of biofilm formation on the samples. The bacterial colonies were counted using the pure-plate technique. Data were analyzed using one-way ANOVA and post hoc Tukey's tests. Regression model was used to assess the association between surface roughness and biofilm formation (P < 0.05). RESULTS: The mean surface roughness of the four groups was not significantly different (P = 0.11); however, a significant difference was noted in the mean biofilm formation among the groups (P = 0.00). CONCLUSION: Bleaching decreased biofilm formation. The lowest biofilm formation was noted in the group subjected to light-activated 35% hydrogen peroxide. Increased surface roughness enhanced biofilm formation to a certain level; excessive roughness did not increase biofilm formation.

Effect of Relining with Different Composite Resins on the Push-out Bond Strength of Anatomical Fiber Posts to Root Canal Dentin.

Introduction: Relining fiber posts using composite resins helps the posts adapt to the anatomical space of the root canals. The aim of this study was to evaluate the effect of composite resin type on the push-out bond strength of anatomical fiber posts to intra canal dentin. Methods and Materials: Forty-eight bovine upper incisors were prepared for post space after root canal therapy and randomly divided in to six groups. Five different types of composite resin (GrandioSO, GrandioSO Heavy Flow, X-tra Fil, X-tra base and everX Posterior) were used for relining the prepared fiber posts. In the control group, the prepared post was cemented into the post space without relining. Cervical, middle and apical cross-sections were achieved from each root and push-out test was conducted at a crosshead speed of 0.5 mm/min. One sample from each group was scanned using a micro-CT scanner which provided views from the apical, middle and coronal thirds. Data were analyzed using Kolmogorov-Smirnov test, repeated measures ANOVA and post hoc tests using SPSS 17 (P<0.05). Results: Statistical analysis showed significant differences in terms of mean push-out bond strengths between different composite resin types and cross-sections (P<0.001). The mean push-out bond strength of the samples relined with Grandio SO composite resin (15.48±2.32) and X-tra Fil Bulk-fill composite resin (14.09±1.98) were significantly higher than that of other groups (P<0.05). The unrelined group had a mean push-out bond strength (5.94±1.45) which was significantly lower than that in other groups (P<0.05). In addition, there was a relationship between cross-sections and composite resin types (P<0.05). Conclusion: This in vitro study showed that the composite resins used for relining can affect the push-out bond strength to intra canal dentin based on their physical and mechanical properties.

Effect of surface preparation with Nd:YAG and Er,Cr:YSGG lasers on the repair bond strength of lithium disilicate glass ceramic to a silorane-based composite resin.

Background. This study was undertaken to evaluate the repair bond strength of lithium disilicate glass ceramic to a silorane-based composite resin after surface preparation with Nd:YAG and Er,Cr:YSGG lasers. Methods. A total of 102 lithium disilicate glass ceramic samples (IPS e.max Press), measuring 5 mm in diameter and 4 mm in thickness, were randomly assigned to 6 groups (n=17): group 1, no surface preparation (control); group 2, acid etching with 9.5% hydrofluoric acid (HF); group 3, surface preparation with 4.5-W Nd:YAG laser; group 4, surface preparation with 6-W Nd:YAG laser; group 5, surface preparation with 1.5-W Er,Cr:YSGG laser; and group 6, surface preparation with 6-W Er,Cr:YSGG laser. After preparation of surfaces and application of silane, all the samples were repaired with the use of a silorane-based composite resin, followed by storage in distilled water at a temperature of 37°C for 24 hours and thermocycling. Finally, the samples were subjected to a shearing bond strength test; the fracture modes were determined under a stereomi-croscope. Results. There were significant differences between the HF group and the other groups (P=0.000). Two-by-two comparisons of the other groups revealed no significant differences (P>0.05). Conclusion. Use of HF proved the most effective surface preparation technique to increase the repair bond strength between lithium disilicate glass ceramic and silorane-based composite resin; compared to the control group.

Effect of smear layer thickness and pH of self-adhesive resin cements on the shear bond strength to dentin.

CONTEXT: There are concerns in relation to the bonding efficacy of self-adhesive resin cements to dentin covered with the smear layer. AIMS: This study aims to evaluate the effect of smear layer thickness and different pH values of self-adhesive resin cements on the shear bond strength to dentin. MATERIALS AND METHODS: The dentin on the buccal and lingual surfaces of 48 sound human premolars were abraded with 60- and 600-grit silicon carbide papers to achieve thick and thin smear layers, respectively. The samples were divided into three groups (n = 16) based on the cement pH: Rely-X Unicem (RXU) (pH < 2); Clearfil SA Luting (CSL) (pH = 3); and Speed CEM (SPC) (pH = 4.5). In each group, composite resin blocks were bonded to the buccal and lingual surfaces. After 24 h, the shear bond strength values were measured in MPa, and the failure modes were evaluated under a stereomicroscope. STATISTICAL ANALYSIS: Data were analyzed with two-way ANOVA and post hoc least significant difference tests (P < 0.05). RESULTS: Cement pH had a significant effect on the shear bond strength (P = 0.02); however, the smear layer thickness had no significant effect on the shear bond strength (P > 0.05). The cumulative effect of these variables was not significant, either (P = 0.11). CONCLUSION: The shear bond strengths of SPC and CSL self-adhesive resin cements were similar and significantly lower than that of RXU. The smear layer thickness was not a determining factor for the shear bond strength value of self-adhesive resin cements.

The effect of repeated preheating of dimethacrylate and silorane-based composite resins on marginal gap of class V restorations.

Background. One of the problems with composite resin restorations is gap formation at resin‒tooth interface. The present study evaluated the effect of preheating cycles of silorane- and dimethacrylate-based composite resins on gap formation at the gingival margins of Class V restorations. Methods. In this in vitro study, standard Class V cavities were prepared on the buccal surfaces of 48 bovine incisors. For restorative procedure, the samples were randomly divided into 2 groups based on the type of composite resin (group 1: di-methacrylate composite [Filtek Z250]; group 2: silorane composite [Filtek P90]) and each group was randomly divided into 2 subgroups based on the composite temperature (A: room temperature; B: after 40 preheating cycles up to 55°C). Marginal gaps were measured using a stereomicroscope at ×40 and analyzed with two-way ANOVA. Inter- and intra-group comparisons were analyzed with post-hoc Tukey tests. Significance level was defined at P < 0.05. Results. The maximum and minimum gaps were detected in groups 1-A and 2-B, respectively. The effects of composite resin type, preheating and interactive effect of these variables on gap formation were significant (P<0.001). Post-hoc Tukey tests showed greater gap in dimethacrylate compared to silorane composite resins (P< 0.001). In each group, gap values were greater in composite resins at room temperature compared to composite resins after 40 preheating cycles (P<0.001). Conclusion. Gap formation at the gingival margins of Class V cavities decreased due to preheating of both composite re-sins. Preheating of silorane-based composites can result in the best marginal adaptation.

Effect of different concentrations of specific inhibitor of matrix metalloproteinases on the shear bond strength of self-adhesive resin cements to dentin.

BACKGROUND: Considering the probability of chemical and enzymatic reactions between matrix metalloproteinases (MMPs) in the dentin structure and their specific inhibitors, the aim of the present study was to evaluate the effect of different concentrations of specific inhibitor of MMPs (galardin) on the shear bond strength of self-adhesive resin cements to dentin. MATERIAL AND METHODS: Forty-eight sound human premolars were mounted in self-cured acrylic resin after removal of the enamel on the buccal and lingual surfaces. The dentin surfaces achieved were polished and prepared with 600-grit silicon carbide paper. The samples were divided into 3 groups (n=16) based on the concentration of galardin used (with no galardin, galardin at a high concentration and galardin at a low concentration). In addition, 96 composite resin blocks, measuring 3 mm in height and diameter, were prepared. The composite resin blocks were bonded to the buccal and lingual surface dentin with Rely-X Unicem (RXC) and Speed CEM (SPC) self-adhesive resin cements, respectively, according to manufacturers' instructions. After 24 hours of storage in distilled water at 37°C, the shear bond strength values were determined in MPa and fracture modes were evaluated under a stereomicroscope. Data were analyzed with two-way ANOVA and post-hoc Bonferroni test (α=0.05). RESULTS: The shear bond strength of galardin at high concentration was significantly higher than that in the control group and galardin at a low concentrations (P<0.001). In addition, galardin at a low concentration exhibited higher shear bond strength compared to the control group (P=0.005). Furthermore, higher shear bond strength values were reported with the use of RXC compared to SPC (P<0.001). CONCLUSIONS: Irrigation with galardin increased the shear bond strength of self-adhesive resin cements to dentin and this increase had a direct relationship with the concentration of galardin in the solution. Key words:N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide, Matrix metalloproteinase inhibitors, Dentin, Extracellular matrix, Luting agents, Dental Bonding.

Effect of different bleaching strategies on microhardness of a silorane-based composite resin.

Background. Dentists' awareness of the effects of bleaching agents on the surface and mechanical properties of restorative materials is of utmost importance. Therefore, this in vitro study was undertaken to investigate the effects of different bleaching strategies on the microhardness of a silorane-based composite resin. Methods. Eighty samples of a silorane-based composite resin (measuring 4 mm in diameter and 2 mm in thickness) were prepared within acrylic molds. The samples were polished and randomly assigned to 4 groups (n=20). Group 1 (controls) were stored in distilled water for 2 weeks. The samples in group 2 underwent a bleaching procedure with 15% carbamide peroxide for two weeks two hours daily. The samples in group 3 were bleached with 35% hydrogen peroxide twice 5 days apart for 30 minutes each time. The samples in group 4 underwent a bleaching procedure with light-activated 35% hydrogen peroxide under LED light once for 40 minutes. Then the microhardness of the samples was determined using Vickers method. Data were analyzed with one-way ANOVA and post hoc Tukey tests (P < 0.05). Results. All the bleaching agents significantly decreased microhardness compared to the control group (P < 0.05). In addition, there were significant differences in microhardness between groups 2 and 4 (P = 0.001) and between groups 3 and 4 (P<0.001). However, no significant differences were detected in microhardness between groups 2 and 3 (P > 0.05). Conclusion. Bleaching agents decreased microhardness of silorane-based composite resin restorations, the magnitude of which depending on the bleaching strategy used.

Shear Bond Strengths of Methacrylate- and Silorane-based Composite Resins to Feldspathic Porcelain using Different Adhesive Systems.

Background and aims. Use of porcelain as inlays, laminates and metal-ceramic and all-ceramic crowns is common in modern dentistry. The high cost of ceramic restorations, time limitations and difficulty of removing these restorations result in delays in replacing fractured restorations; therefore, their repair is indicated. The aim of the present study was to compare the shear bond strengths of two types of composite resins (methacrylate-based and silorane-based) to porcelain, using three adhesive types. Materials and methods. A total of 156 samples of feldspathic porcelain surfaces were prepared with air-abrasion and randomly divided into 6 groups (n=26). In groups 1-3, Z250 composite resin was used to repair porcelain samples with Ad-per Single Bond 2 (ASB), Clearfil SE Bond (CSB) and Silorane Adhesive (SA) as the bonding systems, afterapplication of silane, respectively. In groups 4-6, the same adhesives were used in the same manner with Filtek Silorane composite resin. Finally, the shear bond strengths of the samples were measured. Two-way ANOVA and post hoc Tukey tests were used to compare bond strengths between the groups with different adhesives at P<0.05. Results. There were significant differences in the mean bond strength values in terms of the adhesive type (P<0.001). In addition, the interactive effect of the adhesive type and composite resin type had no significant effect on bond strength (P=0.602). Conclusion. The results of the present study showed the highest repair bond strength values to porcelain with both composite resin types with the application of SA and ASB.

Effect of sodium ascorbate on the bond strength of all-in-one adhesive systems to NaOCl-treated dentin.

BACKGROUND: Ascorbic acid and its salts are low-toxicity products, which are routinely used in food industries as antioxidants. The aim of the present study was to evaluate the effect of 10% sodium ascorbate on the bond strength of two all-in-one adhesive systems to NaOCl-treated dentin. MATERIAL AND METHODS: After exposing the dentin on the facial surface of 90 sound human premolars and mounting in an acrylic resin mold, the exposed dentin surfaces were polished with 600-grit SiC paper under running water. Then the samples were randomly divided into 6 groups of 15. Groups 1 and 4 were the controls, in which no surface preparation was carried out. In groups 2 and 5 the dentin surfaces were treated with 5.25% NaOCl alone for 10 minutes and in groups 3 and 6 with 5.25% NaOCl for 10 minutes followed by 10% sodium ascorbate for 10 minutes. Then composite resin cylinders, measuring 2 mm in diameter and 2 mm in height, were bonded on the dentin surfaces in groups 1, 2 and 3 with Clearfil S3 Bond and in groups 4, 5 and 6 with Adper Easy One adhesive systems according to manufacturers' instructions. The samples were stored in distilled water for 24 hours at 37°C and then thermocycled. Finally, the samples underwent shear bond strength test in a universal testing machine at a strain rate of 1 mm/min. Data were analyzed with two-way ANOVA and post hoc Tukey tests at α=0.05. RESULTS: The differences between groups 1 and 2 (P=0.01), 1 and 5 (P=0.003). 1 and 6 (P=0.03) and 4 and 5 (P=0.03) were statistically significant. Two-by-two comparisons did not reveal any significant difference between other groups (P>0.05). CONCLUSIONS: Use of 10% sodium ascorbate for 10 minutes restored the decreased bond strength of the adhesive systems to that of the control groups. KEY WORDS: Sodium ascorbate, adhesive systems, all-in-one, bond strength, sodium hypochlorite.

Antibacterial Effect of All-in-one Self-etch Adhesives on Enterococcus faecalis.

Background and aims. The aim of this study was to evaluate the antibacterial activity of one-step self-etch adhesives on Enterococcus faecalis on days 1, 7 and 14 with the use of modified direct contact test. Materials and methods. The modified directcontact test was used to evaluate the antibacterial effect of Adper Easy One, Bond Force, Clearfil S3 Bond, Futurabond M, G-Bond, iBond and OptiBond All-in-one adhesives on Enterococcus faecalis after aging the samples in phosphate-buffered saline for one, seven and fourteen days. Data were analyzed using one-way ANOVA and post hoc Tukey tests. Aging effect of each adhesive was evaluated by paired-sample test. In this study, P<0.05 was considered significant. Results. All the tested adhesives exhibited antibacterial activity after one day and had significant differences with the positive control group (P<0.05). After one week, OptiBond All-in-one, iBond and Futurabond M exhibited significant differences in bacterial growth from other groups (P<0.05). There were no significant differences between the groups in two weeks (P>0.05). Conclusion. iBond exhibited the highest antibacterial effect on E. faecalis after one week. Futurabond and OptiBond All-in-one exhibited antibacterial effects against E. faecalis for one week.

Effect of Surface Treatment with Er;Cr:YSSG, Nd:YAG, and CO2 Lasers on Repair Shear Bond Strength of a Silorane-based Composite Resin.

Background and aims. The aim of the present study was to compare the effect ofsurface treatment with Er; Cr:YSSG, Nd:YAG, and CO2 lasers on repair shear bond strength of a silorane-based composite resin. Materials and methods. Sixty eight cylindrical samples of a silorane-based composite resin (Filtek Silorane) were pre-pared and randomly divided into 4 groups as follows: group 1: without surface treatment; groups 2, 3 and 4 with surface treatments using Er; Cr:YSSG, Nd:YAG, and CO2 lasers, respectively. A positive control group (group 5) was assigned in order to measure cohesive strength. Repair shear bond strength values were measured and data was analyzed using one-way ANOVA and a post hoc Tukey test at a significance level of α=0.05. Results. There were statistically significant differences in repair shear bond strength values between group 2 and other groups (P < 0.05); and between group 1and groups 3and 4 (P < 0.001); however, there were no significant differences be-tween groups 3 and 4 (P = 0.91). Conclusion. The repair shear bond strength of silorane-based composite resin was acceptable by surface treatment with lasers.

Effect of 15% carbamide peroxide bleaching gel on color stability of giomer and microfilled composite resin: An in vitro comparison

Objectives: The effect of 15% carbamide peroxide bleaching gel on color stability and surface topography of a giomer and a microfilled composite resin was evaluated in the present in vitro study. Study design: Forty discs measuring 10 mm in diameter and 1 mm in thickness were prepared from a giomer and a microfilled composite resin. Each material yielded 20 discs with completely smooth surfaces. Then a spectrophotometer was used to measure L* (lightness), a* (redness, greenness) and b* (blueness, yellowness) color coordinates of all the discs. Subsequently, the specimens were subjected to 15% carbamide peroxide bleaching gel. After measuring the color coordinates once again, color changes (ÄE*) were calculated by the CIELAB system. Six specimens from each material (three specimens before bleaching agent application and three specimens thereafter) were viewed under an atomic force microscope (AFM) for surface topography evaluation. Data were analyzed by Mann-Whitney U and Kruskal-Wallis tests at á=0.05. Results: There were no statistically significant differences in color changes (ÄE*) between the two materials (P>0.05). In addition, no significant differences were detected in surface roughness between composite resin and giomer discs before and after bleaching (P>0.05 for both). However, in both materials the differences in surface roughness were significant before and after bleaching procedures (P<0.001). Conclusions: Based on the results of the present study it was concluded that 15% carbamide peroxide does not induce clinically detectable color changes in composite resin and giomer despite an increase in surface roughness (AU)

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