RESUMO
Drought is an environmental stressor that affects crop yield worldwide. Understanding plant physiological responses to stress conditions is needed to secure food in future climate conditions. In this study, we applied a combination of plant physiology and metabolomic techniques to understand plant responses to progressive water deficit focusing on the root system. We chose two legume plants with contrasting tolerance to drought, the widely cultivated alfalfa Medicago sativa (Ms) and the model legume Medicago truncatula (Mt) for comparative analysis. Ms taproot (tapR) and Mt fibrous root (fibR) biomass increased during drought, while a progressive decline in water content was observed in both species. Metabolomic analysis allowed the identification of key metabolites in the different tissues tested. Under drought, carbohydrates, abscisic acid, and proline predominantly accumulated in leaves and tapRs, whereas flavonoids increased in fibRs in both species. Raffinose-family related metabolites accumulated during drought. Along with an accumulation of root sucrose in plants subjected to drought, both species showed a decrease in sucrose synthase (SUS) activity related to a reduction in the transcript level of SUS1, the main SUS gene. This study highlights the relevance of root carbon metabolism during drought conditions and provides evidence on the specific accumulation of metabolites throughout the root system.
RESUMO
Se estudian los problemas psicológicos de 9 pacientes con enfermedad de Hansen hospitalizados en el servicio de medicina tropical de HGBCH del Rímac. Se utilizan pruebas psicológicas como: test de Zung, Machover, MMPI, y una historia clínica completa de cada uno. Se parte de la hipótesis básica de que, en nuestro medio, los pacientes con Hansen sufren problemas psicológicos que tienen como base causal la relegación social de que son objeto; se plantea asimismo, que el cuadro predominante es la depresión reactiva. En la investigación se demuestra tal hipótesis, lo que permite establecer que el tratamiento de estos pacientes tiene que ser enfocado en forma integral(AU)
