[Pandemic and value change?] / Pandemie und Wertewandel?: Verschiebungen von Wertprioritäten in der österreichischen Bevölkerung und in einzelnen Wähler*innenschichten als Reaktion auf die COVID-19-Pandemie.

Recent sociological diagnoses suggest that profound social crises such as the COVID-19 pandemic challenge our value orientations and could change them even in the relatively short term. Based on this observation, we investigate whether significant shifts in value priorities according to the Shalom Schwartz scale took place in Austria in the period May 2020 to March/April 2021. The first two waves of the Values in Crisis panel study serve as data material. Two theoretical assumptions are central to the interpretation of the results: first, the thesis of a trend toward conservatism and second, the thesis of the effective power of political discourses in times of (re)emerging populism. The article also pays special attention to a methodological discussion of changes in the meaning of questionnaire items due to the COVID-19 pandemic.The empirical analyses confirm a clear stability of value orientations. Above all, the value of conformity has changed, becoming more important for a significant part of the population; at the same time, the desire for a hedonistic lifestyle lost some of its importance. Conformity became more important, particularly for voters of the governing political parties, while this trend was not apparent, especially among voters of the FPÖ. Since the observed shift in value priorities mainly concerns "pandemic-sensitive" value dimensions, the results suggest a short-term reaction to the crisis rather than a long-term change in values.

Synaptic activity induces dramatic changes in the geometry of the cell nucleus: interplay between nuclear structure, histone H3 phosphorylation, and nuclear calcium signaling.

Synaptic activity initiates many adaptive responses in neurons. Here we report a novel form of structural plasticity in dissociated hippocampal cultures and slice preparations. Using a recently developed algorithm for three-dimensional image reconstruction and quantitative measurements of cell organelles, we found that many nuclei from hippocampal neurons are highly infolded and form unequally sized nuclear compartments. Nuclear infoldings are dynamic structures, which can radically transform the geometry of the nucleus in response to neuronal activity. Action potential bursting causing synaptic NMDA receptor activation dramatically increases the number of infolded nuclei via a process that requires the ERK-MAP kinase pathway and new protein synthesis. In contrast, death-signaling pathways triggered by extrasynaptic NMDA receptors cause a rapid loss of nuclear infoldings. Compared with near-spherical nuclei, infolded nuclei have a larger surface and increased nuclear pore complex immunoreactivity. Nuclear calcium signals evoked by cytosolic calcium transients are larger in small nuclear compartments than in the large compartments of the same nucleus; moreover, small compartments are more efficient in temporally resolving calcium signals induced by trains of action potentials in the theta frequency range (5 Hz). Synaptic activity-induced phosphorylation of histone H3 on serine 10 was more robust in neurons with infolded nuclei compared with neurons with near-spherical nuclei, suggesting a functional link between nuclear geometry and transcriptional regulation. The translation of synaptic activity-induced signaling events into changes in nuclear geometry facilitates the relay of calcium signals to the nucleus, may lead to the formation of nuclear signaling microdomains, and could enhance signal-regulated transcription.

Calcium signals can freely cross the nuclear envelope in hippocampal neurons: somatic calcium increases generate nuclear calcium transients.

BACKGROUND: In hippocampal neurons, nuclear calcium signaling is important for learning- and neuronal survival-associated gene expression. However, it is unknown whether calcium signals generated by neuronal activity at the cell membrane and propagated to the soma can unrestrictedly cross the nuclear envelope to invade the nucleus. The nuclear envelope, which allows ion transit via the nuclear pore complex, may represent a barrier for calcium and has been suggested to insulate the nucleus from activity-induced cytoplasmic calcium transients in some cell types. RESULTS: Using laser-assisted uncaging of caged calcium compounds in defined sub-cellular domains, we show here that the nuclear compartment border does not represent a barrier for calcium signals in hippocampal neurons. Although passive diffusion of molecules between the cytosol and the nucleoplasm may be modulated through changes in conformational state of the nuclear pore complex, we found no evidence for a gating mechanism for calcium movement across the nuclear border. CONCLUSION: Thus, the nuclear envelope does not spatially restrict calcium transients to the somatic cytosol but allows calcium signals to freely enter the cell nucleus to trigger genomic events.