Assuntos
Leucemia Mielomonocítica Crônica/complicações , Xantogranuloma Necrobiótico/complicações , Procedimentos Cirúrgicos de Citorredução , Feminino , Humanos , Leucemia Mielomonocítica Crônica/patologia , Pessoa de Meia-Idade , Xantogranuloma Necrobiótico/patologia , Xantogranuloma Necrobiótico/cirurgiaRESUMO
The development of an assay to measure the human immune response to pneumococcal capsular polysaccharides is described.
Assuntos
Anticorpos Antibacterianos/análise , Vacinas Bacterianas/imunologia , Polissacarídeos Bacterianos/imunologia , Streptococcus pneumoniae/imunologia , Adulto , Ensaio de Imunoadsorção Enzimática , Humanos , VacinaçãoRESUMO
The enzyme-linked immunosorbent assay (ELISA) described by Engvall and Perlmann, which uses antigen-coated tubes and enzyme-labeled anti-immunoglobulin, has been used for the detection of antibodies against streptococcal M protein. The antigen used in the assay was obtained by guanidine extraction of type M-12 streptococcal cell walls followed by hydroxyapatite chromatography. This antigen has the capacity to elicit bactericidal antibodies in rabbits. The results show that the ELISA is specific and highly sensitive for the detection of antibodies in rabbit and human antisera. Preliminary results suggest that, when M-12 antigen is used, the antibodies detected by ELISA are the same antibodies detected in the bactericidal test. The assay has been performed with human and rabbit sera. There was a 96% agreement between bactericidal and ELISA results with rabbit sera and 97.5% agreement with human sera. All bactericidal antibody-positive sera tested thus far yielded positive ELISA results.