RESUMO
Microbe Base is a national computerized database comprising in excess of 1.7 million patient records down-loaded from the laboratory computer systems of 61 participating UK laboratories over 10 years. This paper highlights the antimicrobial susceptibilities of organisms isolated from the urinary tract which comprise around 50% of all isolates in the database. These data may be used to determine trends in antimicrobial susceptibilities; to formulate local antibiotic policies; to compare local with national data and, overall, to assist clinicians in the rational choice of antibiotic therapy and to prevent misuse, or overuse, of antibiotics.
Assuntos
Bactérias/efeitos dos fármacos , Infecções Urinárias/tratamento farmacológico , Humanos , Testes de Sensibilidade Microbiana , Infecções Urinárias/microbiologiaRESUMO
Although several workers have demonstrated that Mycoplasma hominis is responsible for 2 to 20% of cases of septicaemia, reports from Britain do not support this premise--possibly because many of the blood culture systems used fail to sustain growth of this organism. Previous work suggests that this is probably due to the inhibitory effects of sodium polyanethol sulphonate (Liquoid) on M. hominis. In an extension of an in-vitro evaluation of blood culture systems, gelatin was shown to give some protection against the inhibitory effect of polyanethol sulphonate on M. hominis. Of 20 systems evaluated, Becton Dickinson's Supplemented Peptone Broth in their Vacutainer collection system was the most successful at recovering M. hominis.
Assuntos
Mycoplasma/isolamento & purificação , Sangue , Meios de Cultura , Gelatina , Humanos , Mycoplasma/crescimento & desenvolvimento , Sepse/microbiologiaRESUMO
In a pilot study a prototype Malthus 128H microbiological growth analyser was used to study the growth of six commonly encountered urinary pathogens in a range of five different laboratory media. No single medium had been able to show acceptable conductance changes for all bacteria within five hours. Subsequently, the Malthus system, with fastidious anaerobe broth as the culture medium, was used to screen 500 consecutive clinical specimens of urine for significant bacteriuria. After two and a half hours the Malthus system detected 32 out of the 40 true positives and eight of the 44 contaminated urines. Increasing the detection time to five hours permitted the recognition of 39 true positives and 37 contaminated urines.
