RESUMO
Leptadenia pyrotechnica (Forssk.) Decne is growing in Cholistan desert, and is known for its laxative, analgesic, anabolic and astringent, antioxidant, anti-inflammatory, antibacterial, antitumor, hypolipidemic and antiatherosclerotic properties. The present study disclosed the metabolic picture of L. pyrotechnica and validates its folk uses. LP-H fraction constitute 25.79±0.11â mgGAE/g extract and 20.64±0.33â mgRE/g extract of phenolic and flavonoid content, respectively, followed by LP-E (23.15±0.33â mgGAE/g extract and 19.40±0.13â mgRE/g extract), however, LP-E exhibited the highest free radical scavenging (DPPH: 21.05±0.45mgTE/g and ABTS: 68.12±0.53â mgTE/g) and metal reducing (FRAP: 44.93±1.66, CUPRAC: 117.42±1.28â mgTE/g, respectively) activities. The total antioxidant capacity in Phosphomolybdenum assay (1.52±0.14â mmolTE/g) and ferrous ion chelating (11.57±0.29â mgEDTAE/g) activities were observed highest for LP-H extract. In cholinesterase's inhibitory assays, the LP-E and LP-W extracts exhibited inhibitory values as 2.43 and 2.40±0.07â mgGALAE/g extract, respectively against AChE, while against BChE the LP-H displayed the highest value as 5.98±0.44â mgGALAE/g extract. The LP-H fraction also showed the highest inhibition potential (7.72±0.14â mmol ACAE/g and 0.55±0.01â mmol ACAE/g, respectively) against α-glucosidase and α-amylase enzymes, while, in tyrosinase inhibitory assay, all the fractions exhibited significant activities in the range of 59.35±0.29 to 55.18±0.49â mgKAE/g extract. RP-UHPLC/MS analysis of LP-M disclosed the presence of 57 metabolites of various classes. A multivariate analysis and molecular docking study was also carried out to establish relationships between the metabolites and the biological activities, which finally validate the use of L. pyrotechnica as herbal medicine or component nutraceutical, food and cosmetic industry.