Molecular Diversity and Combining Ability in Newly Developed Maize Inbred Lines under Low-Nitrogen Conditions.

Nitrogen is an essential element for maize growth, but excessive application can lead to various environmental and ecological issues, including water pollution, air pollution, greenhouse gas emissions, and biodiversity loss. Hence, developing maize hybrids resilient to low-N conditions is vital for sustainable agriculture, particularly in nitrogen-deficient soils. Combining ability and genetic relationships among parental lines is crucial for breeding superior hybrids under diverse nitrogen levels. This study aimed to assess the genetic diversity of maize inbred lines using simple sequence repeat (SSR) markers and evaluate their combining ability to identify superior hybrids under low-N and recommended conditions. Local and exotic inbred lines were genotyped using SSR markers, revealing substantial genetic variation with high gene diversity (He = 0.60), moderate polymorphism information content (PIC = 0.54), and an average of 3.64 alleles per locus. Twenty-one F1 hybrids were generated through a diallel mating design using these diverse lines. These hybrids and a high yielding commercial check (SC-131) were field-tested under low-N and recommended N conditions. Significant variations (p < 0.01) were observed among nitrogen levels, hybrids, and their interaction for all recorded traits. Additive genetic variances predominated over non-additive genetic variances for grain yield and most traits. Inbred IL3 emerged as an effective combiner for developing early maturing genotypes with lower ear placement. Additionally, inbreds IL1, IL2, and IL3 showed promise as superior combiners for enhancing grain yield and related traits under both low-N and recommended conditions. Notably, hybrids IL1×IL4, IL2×IL5, IL2×IL6, and IL5×IL7 exhibited specific combining abilities for increasing grain yield and associated traits under low-N stress conditions. Furthermore, strong positive associations were identified between grain yield and specific traits like plant height, ear length, number of rows per ear, and number of kernels per row. Due to their straightforward measurability, these relationships underscore the potential of using these traits as proxies for indirect selection in early breeding generations, particularly under low-N stress. This research contributes to breeding nitrogen-efficient maize hybrids and advances our understanding of the genetic foundations for tolerance to nitrogen limitations.

Morpho-physiological mechanisms of two different quinoa ecotypes to resist salt stress.

BACKGROUND: Quinoa (Chenopodium quinoa Willd.) is a facultative halophyte showing various mechanisms of salt resistance among different ecotype cultivars. This study aimed to determine salt resistance limits for a Peruvian sea level ecotype "Hualhuas" and a Bolivian salar ecotype "Real" and elucidate individual mechanisms conferring differences in salt resistance between these cultivars. The plants were grown in sandy soil and irrigated with various saline solutions concentrations (0, 100, 200, 300, 400, and 500 mM NaCl) under controlled conditions. RESULTS: High salinity treatment (500 mM NaCl) reduced the plant growth by 80% and 87% in Hualhuas and Real cultivars, respectively. EC50 (water salinity which reduces the maximum yield by 50%) was at a salinity of 300 mM NaCl for Hualhuas and between 100 and 200 mM NaCl for Real plants. Both cultivars were able to lower the osmotic potential of all organs due to substantial Na+ accumulation. However, Hualhuas plants exhibited distinctly lower Na+ contents and consequently a higher K+/Na+ ratio compared to Real plants, suggesting a more efficient control mechanism for Na+ loading and better K+ retention in Hualhuas plants. Net CO2 assimilation rates (Anet) were reduced, being only 22.4% and 36.2% of the control values in Hualhuas and Real, respectively, at the highest salt concentration. At this salinity level, Hualhuas plants showed lower stomatal conductance (gs) and transpiration rates (E), but higher photosynthetic water use efficiency (PWUE), indicative of an efficient control mechanism over the whole gas-exchange machinery. CONCLUSION: These results reveal that Hualhuas is a promising candidate in terms of salt resistance and biomass production compared to Real.

Foliar-Applied Potassium Silicate Coupled with Plant Growth-Promoting Rhizobacteria Improves Growth, Physiology, Nutrient Uptake and Productivity of Faba Bean (Vicia faba L.) Irrigated with Saline Water in Salt-Affected Soil.

The continuity of traditional planting systems in the last few decades has encountered its most significant challenge in the harsh changes in the global climate, leading to frustration in the plant growth and productivity, especially in the arid and semi-arid regions cultivated with moderate or sensitive crops to abiotic stresses. Faba bean, like most legume crops, is considered a moderately sensitive crop to saline soil and/or saline water. In this connection, a field experiment was conducted during the successive winter seasons 2018/2019 and 2019/2020 in a salt-affected soil to explore the combined effects of plant growth-promoting rhizobacteria (PGPR) and potassium (K) silicate on maintaining the soil quality, performance, and productivity of faba bean plants irrigated with either fresh water or saline water. Our findings indicated that the coupled use of PGPR and K silicate under the saline water irrigation treatment had the capability to reduce the levels of exchangeable sodium percentage (ESP) in the soil and to promote the activity of some soil enzymes (urease and dehydrogenase), which recorded nearly non-significant differences compared with fresh water (control) treatment, leading to reinstating the soil quality. Consequently, under salinity stress, the combined application motivated the faba bean vegetative growth, e.g., root length and nodulation, which reinstated the K+/Na+ ions homeostasis, leading to the lessening or equalizing of the activity level of enzymatic antioxidants (CAT, POD, and SOD) compared with the controls of both saline water and fresh water treatments, respectively. Although the irrigation with saline water significantly increased the osmolytes concentration (free amino acids and proline) in faba bean plants compared with fresh water treatment, application of PGPR or K-silicate notably reduced the osmolyte levels below the control treatment, either under stress or non-stress conditions. On the contrary, the concentrations of soluble assimilates (total soluble proteins and total soluble sugars) recorded pronounced increases under tested treatments, which enriched the plant growth, the nutrients (N, P, and K) uptake and translocation to the sink organs, which lastly improved the yield attributes (number of pods plant-1, number of seeds pod-1, 100-seed weight). It was concluded that the combined application of PGPR and K-silicate is considered a profitable strategy that is able to alleviate the harmful impact of salt stress alongside increasing plant growth and productivity.

Effect of whole body vibration training on bone mineral density and functional capacity in children with thalassemia.

Purpose: To investigate the effectiveness of whole body vibration (WBV) on bone mineral density (BMD) and functional capacity in children with ß-thalassemia major. Methods: Thirty-nine children (23 boys and 16 girls) with ß-thalassemia major, with ages ranging from 6 to 10 years, participated in a randomized controlled trial. They were selected and randomly divided, using computer-generated random numbers into two groups. The control group (n = 20) received the medical treatment and the physical therapy program whereas the study group (n = 19) received the same program as the control group in addition to WBV training (25-30 Hz, 2 mm amplitude, 10 repetitions (30-60 s) with 1 min rest, 15-20 min/day, 3 days/week/24 weeks). Bone mineral density and functional capacity were assessed before and after 24 weeks. Results: Both groups showed a significant increase in BMD and functional capacity after treatment compared with that before treatment (P ˂ 0.05). However, children in the study group demonstrated a significant increase in the functional capacity and the BMD of the lumbar spine, femoral neck and total body compared with that of the control group (P > .01). Conclusions: Whole body vibration may be an effective modality in improving BMD and functional capacity in children with ß-thalassemia major.

Interaction Effects of Nitrogen Source and Irrigation Regime on Tuber Quality, Yield, and Water Use Efficiency of Solanum tuberosum L.

Two field experiments were conducted to investigate the effects of three drip irrigation regimes (G1: 120% crop evapotranspiration (ETc), G2: 100% ETc, and G3: 80% ETc) and four nitrogen (N) source treatments (S0: non-fertilized; S1: urea, S2: ammonium nitrate, and S3: ammonium sulfate on water consumption use, water utilization efficiency, chlorophyll, yield and tubers quality of potato (Solanum tuberosum L.; cv Diamond) under a drip irrigation system during two successive winter seasons (2015/16 and 2016/17)). Nitrogen fertilization was applied at 380 kg ha-1 as standard application for potato in the investigated area. The highest tubers yield was obtained from potato grown with G1 S2 (65.8 Mg ha-1), G1 S3 (63.6 Mg ha-1), G2 S2 (64.1 Mg ha-1), and G2 S3 (62.4 Mg ha-1), while the lowest tubers yield was obtained from potato grown with G3 S0 (10.1 Mg ha-1) and G2S0 (17.4 Mg ha-1). Different treatments of N source resulted in a significant increase for water use efficiency (WUtE) compared with unfertilized treatment. For the interaction effect, the highest WUtE was obtained from potato grown with G3 S2 (18.1 kg m-3), followed by G3 S3 (17.6 kg m-3), while the lowest WUtE was obtained from plants grown with G3S0 (3.0 kg m-3). However, the highest chlorophyll content was obtained from plants grown with G1 and any N source, followed by G2S1-3, while the lowest chlorophyll content was obtained from those grown with G3S0. The highest N, S, protein, and P contents in tubers were obtained from plants grown with G3S3, G3S2, and G2S2, while the highest K content in tubers was obtained from plants grown with G1S1 and G1S2. In concussion, the integrative effects of G1 or G2 with S2 or S3 is recommended for high productivity, while the integrative effects of G3S3 and G3S2 are recommended for high quality tubers.

Effect of twister wrap orthosis on foot pressure distribution and balance in diplegic cerebral palsy.

OBJECTIVES: To evaluate the effectiveness of twister wrap orthosis (TWO) on foot pressure distribution and postural balance in children with spastic diplegic cerebral palsy (CP). METHODS: Thirty children with spastic diplegic CP, with ages ranging from 6 to 8 years, were assigned randomly into two groups. The control group received the conventional physical therapy and ankle foot orthosis (AFO), whereas the study group received the same program as the control group in addition to TWO. Measurement of foot pressure distribution using a pressure platform as well as stability indices using the Biodex Stability System was performed before and after 12 weeks of the treatment program. RESULTS: Both groups showed a significant increase in mean and peak planter pressure on forefoot and rear foot with a significant decrease on mid foot after treatment (P⟨0.05). The study group showed a significant improvement in balance after treatment (P⟨0.05) while there was no significant difference in the control group. After treatment, the study group showed significant improvement in planter pressure and balance compared with the control group (P⟨0.05). CONCLUSIONS: TWO could provide correction of foot pressure distribution and improve postural balance in children with spastic diplegic CP.

Effect of isokinetic training on muscle strength and postural balance in children with Down's syndrome.

Children with Down's syndrome (DS) often have greater postural sway and delay in motor development. Muscle weakness and hypotonia, particularly of the lower extremities, are theorized to impair their overall physical health and ability to perform daily activities. Therefore, the aim of this study was to investigate the effects of isokinetic training on muscle strength and postural balance in children with DS. Thirty-one children with DS ranging in age from 9 to 12 years were assigned randomly into two groups. The control group received the conventional physical therapy, whereas the study group received the same therapy as the control group in addition to the isokinetic training 3 days a week for 12 weeks. Measurement of stability indices using the Biodex Stability System as well as peak torque of knee flexors and extensors of both sides using the isokinetic dynamometer was performed before and after 12 weeks of the treatment program. Each group showed significant improvements in postural balance and peak torque of knee flexors and extensors (P<0.05), with significantly greater improvements observed in the study group compared with the control group (P<0.05). These outcomes indicated that participation in the isokinetic training program induced greater improvements in muscle strength and postural balance in children with DS.

Safety, efficacy, and early clinical experience of insulin degludec in Japanese people with diabetes mellitus: A first-year report from Japan.

In Japan, insulin therapy is recommended for the treatment of type 2 diabetes mellitus either directly after diet, exercise and lifestyle improvements, or if the target for glycemic control is not achieved with other hypoglycemic agents. Insulin degludec is an ultra-long-acting insulin that was launched in Japan in 2013, having shown good efficacy and safety in its clinical development program. It has now been used in clinical practice for more than 1 year. During this time, clinicians and researchers have identified possible factors that could influence the decision as to which patients might be appropriate for insulin degludec treatment. In the present review, we describe how to initiate and manage insulin degludec therapy in routine clinical practice. We also discuss several important topics related to the use of insulin degludec, including patient selection, dosing, handling of bolus insulin, hypoglycemia and other potential safety considerations.

LASER versus electromagnetic field in treatment of hemarthrosis in children with hemophilia.

Children with hemophilia usually have recurrent joint bleeding that leads to joint damage, loss of range of motion, and restriction of mobility, therefore affecting the quality of life in these children. The purpose of this study was to compare the effects of low-level laser therapy (LLLT) to that of pulsed electromagnetic field (PEMF) in treatment of hemarthrosis in children with hemophilia. Thirty boys with hemophilia A with ages ranging from 9 to 13 years were selected and assigned randomly, using sealed envelopes, into two equal intervention groups. The study group I received the traditional physical therapy program in addition to LLLT, whereas the study group II received the same physical therapy program given to the study group I in addition to PEMF. Both groups received the treatment sessions three times per week for three successive months. Pain, laboratory investigations, swelling, and range of motion (ROM) of the affected knee joint, in addition to physical fitness were evaluated before, at the end of the sixth week and at 12 weeks of the treatment program. Laser group showed significant improvement in all measured variables after the sixth week of treatment when compared with PEMF. By 12 weeks of treatment, there was a significant improvement in pain, ROM, ESR and leucocytes levels in laser group compared with PEMF, while there was no significant difference in knee circumferences and the 6-min walk test (6MWT) between both groups. Both groups showed significant improvement at 12 weeks of treatment compared with that at 6 weeks. Both LLLT and PEMF are effective modalities in reducing pain, swelling, increasing ROM and improving physical fitness. Twelve weeks of treatment of both modalities demonstrated significant improvement than 6 weeks of treatment. Laser therapy induced significant improvement than electromagnetic therapy in treatment of hemarthrosis-related problems in children with hemophilia.

Extended curettage and adjuvant therapy for benign tumors of the talus.

PURPOSE: Benign tumors of the talus are rare, and their management is controversial. Recent efforts have extended the safety margin of intralesional excision and curettage by chemical and physical means. Cryotherapy as adjuvant therapy is associated with risks of local wound and bony complications (delayed healing, potential for pathological fractures). METHODS: We retrospectively reviewed six cases of benign talar tumors (three giant cell tumors, two aneurysmal bone cysts, one osteoblastoma) treated by extended curettage and adjuvant cryotherapy. Talar bone stock was restored by bone grafting the residual cavity with fibular strut grafts and/or cancellous bone grafts. Patients were followed for a mean of 40 months. RESULTS: At latest follow-up, each patient had a mobile painless ankle and good functional outcome. No patient experienced recurrence or complications related to cryotherapy. CONCLUSIONS: Intralesional extended curettage with bone grafting and cryotherapy is a successful, safe, and efficient treatment of benign tumors of the talus.

Screening for developmental dysplasia of the hip in Hong Kong.

PURPOSE: To review records of children referred to our hospital for developmental dysplasia of the hip (DDH) and evaluate the effectiveness of hip screening in Hong Kong. METHODS: Records of children suspected of DDH and referred to our hospital between 1 January 2005 and 31 March 2010 were reviewed. The diagnosis was based on static and dynamic ultrasonography of the hips and/or radiographic features of hip subluxation, dislocation, and pelvic dysplasia. The age of the children at presentation was recorded. Late diagnoses were those presenting after the age of 6 months. Late presentations were those presenting after the walking age. RESULTS: Of 213 referred children, 33 were diagnosed to have DDH. Their mean age at presentation was 90.8 (range, 9-559) days. The female-to-male ratio was 2.9:1, and the mean follow-up duration was 25.1 (range, 4.2-50.3) months. 30 of the DDH infants were diagnosed before the age of 6 months, one at 8 months, and 2 were late presenters. Hence, the failure rate of the hip screening was 0.09 per 1000 live births. The remaining 180 were confirmed to be normal and followed up until the walking age. The point prevalence of DDH on Hong Kong Island was 0.87/1000 live births. CONCLUSION: In light of the low point prevalence of DDH in Hong Kong born children, the protocol of universal clinical assessment and selective ultrasonographic screening of suspected cases is justified.

Minimally invasive, no hardware subtalar arthrodesis with autogenous posterior iliac bone graft.

Sixteen patients underwent minimally invasive subtalar arthrodesis through a mini-invasive approach with posterior iliac graft between 2004 and 2006. No hardware was used to transfix the arthrodesis and partial weight bearing was allowed immediately. The primary indication for surgery was the squeal of fracture os calcis in terms of subtalar joint arthritis, loss of heel height, malalignment of the hindfoot, and pain with weight bearing. There were 12 male and 4 female patients with a mean age of 30 (range 17-52). Patients were followed up for a period of 40.8 months (range 36-48 months). The mean interval from injury to fusion was 2 (+0.6) years ranging from 6 months to 6 years post fracture. The average clinical rating scale based on the American Orthopaedic Foot and Ankle Society (AOFAS) improved from 36 preoperatively to 78 at the latest follow-up (P < 0.05). Union rate was 94%. Radiographic evaluation revealed a mean increase in calcaneal inclination of 6.25 + 8.3 degrees (P < 0.07) and a mean increase in the lateral talocalcaneal angle of 7.42 + 10.2 degrees (P < 0.08). Complications were graft nonunion in 1 patient and transient tendoachilles tendinitis in another. This technique can be used to decrease the morbidity associated with the late complications of os calcis fractures by aligning the hindfoot, restoring the heel height and correcting calcaneal and talar inclination. It offers the advantage of early weight bearing while avoiding hardware complications.