[Heterotopic ossifications: a severe complication following extensive burn injury]. / Heterotope Ossifikationen - eine schwerwiegende Komplikation bei ausgedehnten Brandverletzungen.

Heterotopic ossifications in periarticular tissue can appear after severe head injury, spine trauma or local joint trauma. Following extensive burns, heterotopic ossifications are a rare, severe complication with an unclear pathogenesis. We report one case with this kind of complication in a 50-year old male patient who sustained full and partial thickness burn injuries over 60 % of the body. The thermal injury was accompanied by a severe inhalation injury.

Effective object recognition for automated counting of colonies in Petri dishes (automated colony counting).

Determination of the number of colonies (colony forming units, CFU) is a standard method in microbiological analysis to ensure the quality of drinking water. Normally this tedious work is still performed manually. A PC-based method for the automated counting of digitized images of Petri dishes is presented. The method includes highly specific and effective object recognition algorithms that ensure very high detection accuracy. The processing sequence implies internal controls therefore enabling reliable automated evaluations of series of images. Use of the Fuzzy formalism and the high adaptivity of the algorithms lead to an extraordinary user-friendliness. For digitization different devices like flatbed scanners or CCD-cameras can be used. Due to the highly adaptive algorithms samples from the routine standard preparation process in laboratories can be evaluated. The accuracy and quality of the method aim at advancement in objectivity of colony counting and quality control and assurance. The algorithms and the evaluation of the method are presented.

Reflection-optical multispectral imaging method for objective determination of burn depth.

We present a noninvasive imaging method for objective determination of the depth of burn wounds. The method is easy to use and enables even the nonspecialized physician to determine the burn depth at a very early time and to make available an objective documentation for quality management.

[Ultrasound morphology of the healthy and diseased hand]. / Die Sonomorphologie der gesunden und der kranken Hand.

Ultrasonography of the hand is rarely applied, so normal and pathological findings remain unfamiliar to daily routine. Although all anatomical structures can be identified by this method, the median and ulnar nerves may be difficult to demonstrate, so they are often identified only by exclusion. Ultrasound is a non-invasive method and very helpful in diagnosing pathological changes, such as ganglions and other tumors, synovialitis, carpal tunnel syndrome, tendon injuries, and anatomical malformations.

Protective immunity linked with a distinct developmental stage of a filarial parasite.

Repeated low dose infections of the jird Meriones unguiculatus, with the filarial parasite Acanthocheilonema viteae cause a substantial reduction of the total worm burden, suggesting a parasite-driven immune mechanism that controls super-infections. Quantitative recovery of parasites from tissues of triple infected jirds reveals that the larvae derived from a subsequent challenge infection are inactivated or severely impaired several days after transmission, precisely during their molt from the L3 to the L4 stage. Moreover, only larvae undergoing the molt from L3 to L4 stages are capable of stimulating an immune response directed against the challenge infection, indicating that protective Ag are produced during the molting period. Consistent with this, inactivated L3 or live L4 do not produce the same effect. In contrast to susceptible animals, immune jirds elicit high serum antibody titers against molting Ag. Indirect fluorescence antibody-binding tests with sera from protected jirds reveal specific labeling of the surface of molting L3 and not other larval stages, implying a stage-specific elimination process. The identification of molting L3 as a natural target for host immune mechanisms, emphasizes the central importance of this larval stage for future efforts aimed toward the development of a filarial vaccine.

[Hibernoma of the forehead. A rare tumor of brown fatty tissue in an unusual site]. / Hibernom der Stirn. Ein seltener Tumor des braunen Fettgewebes in ungewöhnlicher Lokalisation.

We report a hibernoma located on the forehead in a 52-year-old female patient. The hibernoma is a rare tumour derived from brown adipose tissue. Location on the forehead is unusual and has not been reported before.

Changes in the surface composition after transmission of Acanthocheilonema viteae third stage larvae into the jird.

This study describes the dynamics and the biochemical nature of changes in the surface of the filarial nematode Acanthocheilonema viteae after its transmission into the vertebrate host. Vector-derived third-stage larvae (mL3) were inoculated into naive Meriones unguiculatus and recovered from the tissues at different times post-infection until their moult to fourth-stage larvae (L4). Surface-specific labelling with fluoresceinated lectins revealed that the larvae are covered by a carbohydrate envelope. Although the mL3 envelope was strongly reduced one day after transmission, new surface carbohydrates appeared until the onset of moulting, some of which could also be identified on the surface of L4. In general, surface carbohydrates were partially shed by moving larvae, suggesting a loose association of these components in the epicuticle. The fate of cuticular lipids and proteins of L3 and L4 was monitored by external 125I-labelling and differential extraction of the components. Thin-layer chromatography of surface-labelled lipids revealed only minor changes 1 day after parasite transmission. Afterwards the number of lipids accessible to label decreased further until moulting was complete. Two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis of surface-labelled proteins showed a consistent surface exposure of mL3 specific proteins until 1 day post-infection. Thereafter, the composition of surface-labelled proteins changed rapidly, resembling that of the L4 as early as several days before moulting. During this period individual differences in the composition of surface proteins were evident.

Recovery, distribution, and development of Acanthocheilonema viteae third- and early fourth-stage larvae in adult jirds.

Living third- and fourth-stage larvae (L3 and L4) of Acanthocheilonema viteae were recovered quantitatively from adult Meriones unguiculatus within the first 10 days after subcutaneous inoculation of 60 arthropod-derived larvae (mL3). The average recovery of the inoculated larvae was about one third (28.5%), and the majority (87.7%) were found in muscular tissues. Seventy-two hours after inoculation, larvae could be isolated from all body locations, although the majority still was found near the site of inoculation. Morphological and biometrical data indicated that, at least until molting, the development of the larval population was not synchronous, with molting occurring over a period of 48 hr on days 7 and 8 postinoculation. The stomatal rings of postinvasive L3's and L4's were distinguishable structurally and could be used as stage-specific determinants. Immediately after infection, L3's showed a linear growth in diameter; rapid longitudinal growth started after the molt, leading to a doubling in the length of L4's within 4 days. The time course of shedding was reconstructed in detail using isolated L3/L4 intermediates.