RESUMO
The diuretic activity of ethanolic extract of Panicum repens was investigated in rats. A single oral dose of 500 mg/kg of P. repens extract were given to rats, after 24 h, urine volume, its sodium and potassium concentrations were estimated. Treatment with P. repens extract caused a significant increase in tested parameters as compared to their corresponding controls, p < 0.05.
Assuntos
Diuréticos/farmacologia , Panicum/química , Extratos Vegetais/farmacologia , Raízes de Plantas/química , Rizoma/química , Administração Oral , Animais , Diuréticos/administração & dosagem , Diuréticos/química , Avaliação Pré-Clínica de Medicamentos/métodos , Etanol/química , Masculino , Extratos Vegetais/análise , Extratos Vegetais/química , Potássio/urina , Ratos Wistar , Sódio/urinaRESUMO
The hypolipidemic effect of an ethanolic extract from the roots and rhizomes of Panicum repens L. was investigated in rats suffering from high-cholesterol, diet-induced hyperlipidemia, and the phytochemicals in the extract were analyzed. The extract was administered p.o. in doses of 250 mg/kg/day together with cholesterol at a dose of 100 mg/kg/day for 7 weeks. The high-cholesterol diet caused a significant increase in total lipids, total cholesterol (TC), total triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and the atherogenic index, whereas the level of high-density lipoprotein cholesterol (HDL-C) was significantly decreased. Administration of the P. repens extract (p<0.05) significantly reduced the rise of the serum levels of total lipids, TC, TG, and LDL-C, as well as the atherogenic index, whereas it significantly increased (p<0.05) the level of HDL-C. HPLC analysis of the phenolics and flavonoids in the extract revealed the presence of gallic acid, chlorogenic acid, chicoric acid, primulic acid, rutin, apigenin-7-glucoside, and quercetin. In conclusion, the P. repens extract was found to possess hypolipidemic activity in high-fat, diet-induced hyperlipidemic rats.
Assuntos
Hiperlipidemias/tratamento farmacológico , Panicum/química , Extratos Vegetais/uso terapêutico , Raízes de Plantas/química , Rizoma/química , Animais , Colesterol na Dieta/administração & dosagem , Flavonoides/análise , Hipolipemiantes/uso terapêutico , Testes de Função Renal , Lipídeos/análise , Lipídeos/sangue , Fígado/química , Testes de Função Hepática , Masculino , Camundongos , Fenóis/análise , Extratos Vegetais/farmacologia , Ratos , Ratos WistarRESUMO
OBJECTIVE: The present study was undertaken to investigate the effect of Tribulus alatus extracts on free serum testosterone in male rats. MATERIALS AND METHODS: Free serum testosterone level was measured in male rats treated with alcoholic extracts of the aerial part without fruits, fruits of Tribulus alatus and their fractions. RESULTS: All tested extracts showed significant increase in the level of free serum testosterone when compared to that of corresponding control, p < 0.05. Statistical comparison of all groups revealed that the maximum level was found in groups treated with chloroformic and ethanolic fractions of fruits extract. CONCLUSION: Tribulus alatus extract appears to possess aphrodisiac activity due to its androgen increasing property.
Assuntos
Afrodisíacos/farmacologia , Fitoterapia , Componentes Aéreos da Planta/química , Comportamento Sexual Animal/efeitos dos fármacos , Testosterona/sangue , Tribulus/química , Animais , Afrodisíacos/química , Avaliação Pré-Clínica de Medicamentos , Masculino , Pênis/irrigação sanguínea , Extratos Vegetais/farmacologia , Distribuição Aleatória , Ratos , Ratos WistarRESUMO
OBJECTIVE: The present study was undertaken to investigate the effect of Tribulus alatus extracts on free serum testosterone in male rats. MATERIALS AND METHODS: Free serum testosterone level was measured in male rats treated with alcoholic extracts of the aerial part without fruits, fruits of Tribulus alatus and their fractions. RESULTS: All tested extracts showed significant increase in the level of free serum testosterone when compared to that of corresponding control, p < 0.05. Statistical comparison of all groups revealed that the maximum level was found in groups treated with chloroformic and ethanolic fractions of fruits extract. CONCLUSION: Tribulus alatus extract appears to possess aphrodisiac activity due to its androgen increasing property.
Assuntos
Animais , Masculino , Ratos , Afrodisíacos/farmacologia , Fitoterapia , Componentes Aéreos da Planta/química , Comportamento Sexual Animal/efeitos dos fármacos , Testosterona/sangue , Tribulus/química , Afrodisíacos/química , Avaliação Pré-Clínica de Medicamentos , Pênis/irrigação sanguínea , Extratos Vegetais/farmacologia , Distribuição Aleatória , Ratos WistarRESUMO
Six steroidal glycosides (1-6) were isolated from the aerial parts of Tribulus alatus Del. (Zygophyllaceae), together with one known cholestane, one spirostane, and six flavonol glycosides. Among them, 1 and 2 possess a furostane-type aglycone, 3 and 6 a cholestane structure, and 4 and 5 a spirostane skeleton. Their structural elucidation was accomplished by extensive spectroscopic methods including 1D ((1)H, (13)C, (13)C DEPT, TOCSY, ROESY) and 2D NMR experiments (DQF-COSY, HSQC, HMBC) as well as ESI-MS analysis.
