Fate mapping of Trps1 daughter cells during cardiac development using novel Trps1-Cre mice.

Tricho-rhino-phalangeal syndrome (TRPS) is a rare congenital disorder that is characterized by abnormal hair growth and skeletal deformities. These result in sparse hair, short stature, and early onset of joint problems. Recent reports have shown that a relatively high proportion of patients with TRPS exhibit a broad range of congenital heart defects. To determine the regulation of Trps1 transcription in vivo, we generated novel transgenic mice, which expressed Cre recombinase under the murine Trps1 proximal promoter sequence (Trps1-Cre). We crossed these mice with Cre reporter mice to identify Trps1 daughter cells. Labeled cells were observed in the appendicular joint tissue, dermal papilla of the hair follicles, cardiac valves, aortic sinus, atrial walls, and the interventricular septum. In situ analysis showed restricted Trps1 expression, which was observed in endocardial cushions of the outflow tract, and in leaflets of all mature cardiac valves. These results suggest that the Trps1 proximal promoter sequence contains some of the tissue-specific Trps1 regulatory region. Further, our findings partially explain why patients with TRPS show a broad range of congenital cardiac defects, although Trps1 expression is observed in a more restricted fashion. genesis 54:379-388, 2016. © 2016 Wiley Periodicals, Inc.

Equine anatomedia: development, integration and evaluation of an e-learning resource in applied veterinary anatomy / Anatomedia equina: desarrollo, integración y evaluación de un recurso e-learning en anatomía veterinaria aplicada

In Egypt, there is a growing movement to encourage veterinary student-centered learning using the most up to date educational technologies. This paper focuses on a computer-facilitated learning program "Equine Anatomedia", which comprises now two modules (head and digit) fully integrated with the applied anatomy curriculum at Alexandria and Damanhour Universities. The educational design of this program allows students and clinicians to explore anatomical concepts, principles and procedure guidelines in a manner more suited to their individual learning needs than traditional methods. The program comprises over 300 high quality images and diagrams, audio and video clips as well as animated graphics with colored keys highlighting the anatomical features. Staff and student feedback indicates that Equine Anatomedia is an effective and engaging learning tool which helps students to develop their knowledge in anatomy and to appreciate its relevance in clinical situations. In addition, it encourages student-staff interaction and is a useful tool in overcoming the challenges of limited resources and increasing numbers of students.

En Egipto, hay un movimiento creciente para fomentar el aprendizaje centrado en el estudiante de veterinaria utilizando las más modernas tecnologías educativas existentes a la fecha. Este trabajo se centra en un programa de aprendizaje por computación denominado "Anatomedia de Equinos", que en la actualidad está conformado de dos módulos (cabeza y dedos) totalmente integrados con el plan de estudios de la asignatura Anatomía Aplicada en las Universidades de Alejandría y Damanhour, Egipto. El diseño educativo de este programa permite a los estudiantes y los médicos clínicos explorar los conceptos anatómicos, como así también principios y directrices de procedimiento relacionados a sus necesidades individuales de aprendizaje, de una manera más adecuada que los métodos tradicionales de aprendizaje. El programa cuenta con más de 300 imágenes de alta calidad y diagramas, audios y vídeos, así como gráficos animados con identificaciones de colores que destacan las características anatómicas. La retroalimentación del personal a cargo del programa y de parte de los estudiantes indica que Anatomedia de Equinos es una herramienta de aprendizaje eficaz y atractiva que ayuda a los estudiantes a desarrollar sus conocimientos en anatomía y les permite apreciar su importancia en situaciones clínicas. Además, el programa permite fomentar la interacción alumno-personal y constituye una herramienta útil que ayuda a superar los retos que ocasionan los recursos limitados y el número creciente de estudiantes.

Morphological variations of the vallate papillae in some mammalian species.

The morpho-structural characteristics of the vallate papillae of the tongue of rat, dog, donkey and buffalo were investigated by macroscopy and their microstructure by light and scanning electron microscopy (SEM). The numbers of vallate papillae varied among the different species. In rat, a single vallate papilla surrounded by incomplete groove and an annular fold was observed. Taste buds were detected along the entire length of the medial and lateral groove epithelium, but not in the papillary dome. In dog, some papillae lacking the annular pad had irregular ridges and grooves toward the center of the papillary surface, while other papillae had small secondary papillary grooves arising from the center of the papilla. Taste buds were located in the medial and lateral epithelium of both primary and secondary grooves as well as in the dome epithelium. In donkey, two papillae were frequently observed around the midline of the tongue root, and an additional papilla was found occasionally in the middle and associated with secondary papilla. In buffalo, several papillae were relatively small and variable in shape. With SEM, small ridges and grooves were found in the papillae of donkey and buffalo. In both species, taste buds were constantly observed along the medial wall epithelium, but no taste buds were found in the lateral wall. We conclude that the vallate papillae exhibited peculiar characteristics, which are species specific and might have a correlation with the variable feeding habits among these animals.