The Influence of Chicken Egg Lysozyme or Zinc-Bacitracin Antibiotic on the Growth Performance, Antibacterial Capacity, Blood Profiles, and Antioxidative Status of Rabbits: A Comparative Study.

Dietary egg lysozyme has beneficial roles in the growth performance and health conditions of animals. The study was performed using 90 multicolored rabbits in three groups (each replicate with thirty rabbits). In the control group, rabbits were fed a diet without zinc bacitracin (ZnB) or egg lysozyme, while the second and third groups were treated with ZnB and lysozyme additive at 100 mg/kg, respectively. After eight weeks, the final weight and body weight gain (BWG) of rabbits fed dietary egg lysozyme and ZnB additives were meaningfully increased (p < 0.05). Nevertheless, the feed conversion ratio (FCR) was markedly decreased by dietary egg lysozyme and ZnB (p < 0.05). Interestingly, dietary egg lysozyme resulted in higher final weight and BWG and lower FCR than rabbits treated with ZnB (p < 0.05). Rabbits treated with egg lysozyme and ZnB additives had markedly lower populations of Clostridium spp. and Escherichia coli (p < 0.05) compared with the control. However, the counts of Lactobacillus and total bacteria were meaningfully increased in the the intestines of rabbits treated with egg lysozyme and ZnB (p < 0.05). The blood total protein and globulin of rabbits fed dietary egg lysozyme and ZnB additives were meaningfully increased (p < 0.05). Blood creatinine was significantly lowered by dietary egg lysozyme compared with the control and ZnB-treated rabbits (p < 0.05). The levels of blood urea, ALT, and AST were markedly lowered (p < 0.05) by dietary egg lysozyme and ZnB. The gene expressions of superoxide dismutase 1 (SOD1) and glutathione peroxidase (GPX) in the liver of rabbits fed dietary egg lysozyme and ZnB additives were markedly upregulated (p < 0.05) compared with the control. Dietary egg lysozyme resulted in higher expression of SOD1 and GPX genes than rabbits treated with ZnB (p < 0.05). In conclusion, the inclusion of egg lysozyme could replace the inclusion of ZnB in the diets of rabbits.

Aspergillus awamori positively impacts the growth performance, nutrient digestibility, antioxidative activity and immune responses of growing rabbits.

In this study, we explored the effects of dietary administration of Aspergillus awamori on the growth, intestinal histomorphology, immune parameters and antioxidant enzyme activity of growing rabbits. The rabbits of 5 weeks of age (body weight, 855 ± 5.53 g) were allotted into four groups (12 rabbits per group) and fed four experimental diets enriched with A. awamori at 0, 50, 100 and 150 mg per kg diet for 8 weeks. After the feeding trial, an equal number of male APRI rabbits (3 months old; 4 groups, 5 rabbits per group) were housed in metabolism cages and fed the test diets for 14 days for the digestibility trial. All A. awamori-enriched diets induced a significant increase in the average body weight and weight gain of rabbits and significantly decreased the feed conversion ratio. In rabbits fed with A. awamori at 100 or 150 mg per kg diet, protein, lipid and fibber digestibility coefficients significantly increased compared with the control group. Intestinal villi measurements (length and thickness) were also enhanced in all rabbits fed with A. awamori. Adding A. awamori as a food supplement generally did not affect rabbit haematology and blood biochemistry values; however, at 150 mg per kg diet, it significantly increased the levels of haemoglobin and total protein, as well as red blood cell count. Furthermore, all enriched diets significantly increased rabbits' phagocytic activity and their phagocytic index. Rabbits fed with A. awamori also showed decreased malondialdehyde but increased catalase activity. In conclusion, A. awamori administered as feed supplement at 100-150 mg per kg of growing rabbits' diet enhances their growth, intestinal health and nutrient digestibility, and it raises the levels of their immune and antioxidative responses.

The Influence of Dietary Chicken Egg Lysozyme on the Growth Performance, Blood Health, and Resistance Against Escherichia coli in the Growing Rabbits' Cecum.

The dietary chicken egg lysozyme (LZM) at different concentrations was tested on the growth performance, blood health, and resistance against Escherichia coli of growing rabbits. A total number of 48 rabbits averaged 611.25 g (5 weeks of age) of APRI line-rabbits (Egyptian developed line) were allocated into four treatments (three replicates and each contained four rabbits) of 5-week weaning APRI rabbits. The first group was fed a basal diet without LZM supplementation and served as a control group, whereas the remaining groups of rabbits were fed a basal diet supplemented with LZM at 50, 100, and 200 mg/kg diet, respectively, for 8 weeks. The obtained results revealed that rabbits fed the basal diet supplemented with different concentrations of LZM linearly (P < 0.05) displayed improved growth performance and reduced feed intake and FCR. The best result was for rabbits fed a 200 mg per kg diet supplemented with LZM, followed by a 100 mg per kg diet. The total count of Escherichia coli and Clostridium count was linearly (P < 0.05) decreased by adding LZM at 100 and 200 mg/kg in the diets compared to the control groups. In contrast, total bacterial count and the total count of Lactobacilli had increased considerably by increasing LZM at different levels relative to the control groups. The LZM supplementation linearly (P < 0.05) increased hematological parameters (RBCs, PCV, Hb, and WBCs) together with an increase in lymphocyte count compared to the control group. The total protein and globulin concentrations were significantly (P < 0.05) increased by feeding with LZM. On the other hand, ALT, AST, urea, and creatinine were significantly (P < 0.05) decreased by increasing LZM supplementation. It could be concluded that supplementation of the rabbit's diet with chicken egg LZM was able to improve the growth performance and hematological and serum biochemical parameters compared with the control group. Therefore, LZM is required at the rate of the hobx100-200 mg/kg diet as a potential feed additive and a friendly alternative for antibiotics in rabbit feed.

Oxidative stress, hemato-immunological, and intestinal morphometry changes induced by ochratoxin A in APRI rabbits and the protective role of probiotics.

This work studied the protective effects of Aspergillus awamori against ochratoxin A (OTA)-induced toxicity in APRI maternal line rabbits. A total number of 48 APRI line weanling rabbits (5 weeks) were divided into 4 groups (12 rabbits each) and fed the basal diet, 30 ppb/kg diet of OTA, 1 g/kg diet of A. awamori, and a mixture of OTA and A. awamori for 8 weeks. OTA reduced the final body weight and weight gain as well as the intestinal villi length and thickness, whereas increased the feed intake and feed conversion ratio. Rabbits fed diets with OTA showed significantly reduced crude protein, lipids, and fibers apparent digestibility coefficients (P < 0.05). The red blood cells and hemoglobin were significantly decreased in the OTA group comparing with the other groups (P < 0.05). The blood total protein and albumin displayed significantly lower levels by OTA than the other groups. In contrast, glucose, aspartate aminotransferase (AST), alkaline phosphatase (ALP), urea, and creatinine levels were significantly increased by OTA (P < 0.05). Phagocytic activity (PA) and phagocytic index (PI) showed significantly (P < 0.05) decreased levels in OTA-contaminated group, while rabbits fed A. awamori significantly showed the highest PA and PI levels (P < 0.05). Dietary A. awamori kept the levels of PA and PI in rabbits fed OTA significantly higher than those fed without A. awamori (P < 0.05) and not significantly different from the control group (P > 0.05). Catalase (CAT) and superoxide dismutase (SOD) displayed significantly lower levels in the OTA group, while malondialdehyde (MDA) was significantly higher than the other groups (P < 0.05). Rabbits fed OTA-contaminated diets displayed significantly lower CAT and SOD and higher MDA than rabbits fed OTA combined with A. awamori (P < 0.05). Our results indicated that dietary A. awamori ameliorated the damage in APRI rabbits fed OTA through alleviation of oxidative stress and immunity.

In ovo injection of nano-selenium spheres mitigates the hatchability, histopathology image and immune response of hatched chicks.

In ovo injection of nano-selenium (Se) produced by lactic acid bacteria (LAB-nano-Se) was investigated on the hatchability, immune responses and the histopathological alterations in hatched chicks. The eggs (18 day age) were injected with 0.5 ml of 0.9% NaCl (normal saline, NS), while the control group was kept without injection. In the third, fourth and fifth groups, the eggs were injected with 0.5 ml of NS and LAB-nano-Se at 10, 20 and 30 µg/egg. The results revealed improved growth performance in groups injected with LAB-nano-Se when compared to the control treatment. The highest final weight and weight gain were noticed in 20 µg LAB-nano-Se/egg group (p < .05). The feed conversion ratio was reduced in all treated groups when compared to the control group (p < .05). Groups injected with LAB-nano-Se showed enhanced hatchability of the whole incubated eggs (p < .05). Total lipids and cholesterol levels were decreased significantly in groups treated with LAB-nano-Se at 10 and 20 µg/egg when compared to the non-treated group. At the same time, globulin was increased by LAB-nano-Se in ovo injection. Furthermore, the total antioxidant capacity, catalase, glutathione peroxidase, superoxide dismutase increased in groups treated with LAB-nano-Se at 10 and 20 µg/egg with insignificant (p > .05) differences with those treated with LAB-nano-Se at 30 µg/egg using in ovo injection technique. Also, higher total blood protein and phagocytosis were significantly observed in groups treated with at 10, 20 and 30 µg LAB-nano-Se/egg. The histopathological images of hatched chicks revealed that nano-Se presented normal effects on liver and kidney tissues and restored the parameters as mentioned earlier. To conclude, LAB-nano-Se exhibited beneficial effects in hatched chicks through improving immune and antioxidant activities as well as histopathological effects by using in ovo technique.

Dietary Moringa oleifera improves growth performance, oxidative status, and immune related gene expression in broilers under normal and high temperature conditions.

The aim of this study is to evaluate the effects of Moringa oleifera (MO) on the performance, antioxidative status, and immune related gene expression in broilers raised under normal or heat stress conditions. Broiler chickens were distributed into 4 groups and fed diets with dietary MO at 0% or 5% (MO0 or MO5) and raised under ambient temperature 22 ±â€¯1 °C (N) or 35±1 °C (HS). HS conditions negatively affected the weight gain and FCR, while feeding MO exhibited beneficial effects especially under HS conditions. Triglycerides, total cholesterol and low-density lipoprotein cholesterol (LDL) levels were significantly (P < 0.05) higher in chickens raised in HS conditions and fed the basal diet than those in normal condition and fed with or without MO, while MO decreased triglycerides and total cholesterol levels in normal and HS conditions. Blood high-density lipoprotein cholesterol (HDL) was significantly decreased in broilers raised in HS conditions and fed diets without MO, while MO increased HDL level. Blood glutathione peroxidase (GSH-Px) was significantly (P < 0.05) decreased in broilers raised in HS conditions and fed the basal diet without MO. mRNA expression of GSH-Px was significantly (P < 0.05) downregulated in broilers raised in HS conditions and fed diets without MO. Broilers under normal or HS conditions and fed the basal diet exhibited significantly (P < 0.05) downregulated mRNA expressions of superoxide dismutase (SOD) and catalase (CAT) compared to chickens under normal conditions and fed MO. Liver and muscle thiobarbituric acid reactive substance (TBARs) were significantly (P < 0.05) increased in broilers under HS conditions and fed diet without MO. The expressions of interleukins (IL2 and IL6) were significantly (P < 0.05) downregulated in broilers under normal or HS conditions and fed diets without MO. To sum up, HS conditions depressed the performance, antioxidative status, and immune related gene expression in broilers, while MO obviously alleviated these negative effects in broilers.

Effects of Dietary Nano-Selenium Supplementation on Growth Performance, Antioxidative Status, and Immunity in Broiler Chickens under Thermoneutral and High Ambient Temperature Conditions.

The objective of the present study was to examine the effects of nano-selenium on growth performance, antioxidative status, and immune function in broiler chickens reared under thermoneutral (22±1°C) or high ambient temperature (35±1°C) conditions. Thirty-six broiler chicks at 15d old were randomly divided into 6 treatments in a 3×2 factorial design. The main factors included the dietary supplementation (basal diet without Se supplementation [control], basal diet with 0.3 mg of nano-elemental Se per kilogram of diet [nano-Se], and basal diet with 0.3 mg of sodium selenite per kilogram of diet [SSe]) and the ambient temperature challenge (22±1°C or 35±1°C). The birds were given the experimental diets from 15 to 30 d of age. High ambient temperature significantly depressed body weight gain, feed intake, feed conversion ratio, breast muscle weight, and abdominal fat weight, while feeding nano-Se clearly alleviated these negative effects of high ambient temperature. In addition, feeding nano-Se increased glutathione peroxidase mRNA expression in liver and alleviated the negative effects of high ambient temperature via reducing the malondialdehyde content in liver and breast muscle. Furthermore, feeding nano-Se increased mRNA expression of cytokine genes (interleukins 2 and 6) under both thermoneutral and high ambient temperature conditions. Under both thermoneutral and high-temperature conditions, broiler chickens fed nano-Se had higher Se and vitamin E concentrations in breast muscle than broiler chickens fed the control diet. In contrast, feeding SSe at the same dose as nano-Se did not alleviate the negative effects of high ambient temperature on broiler chickens. In conclusion, dietary supplementation with nano-Se at 0.3 mg/kg diet might enhance growth performance by improving antioxidative or immune properties in broilers reared under high ambient temperature.