RESUMO
Cysticercus tenuicollis, the larval stage of Taenia hydatigenia, infects sheep and causes economic losses due to condemnation of infected organs. This study was designed to report the infection rate, risk factors, biochemical, and molecular characterization of Cysticercus tenuicollis in sheep from Ashmoun, Minoufiya, Egypt. The infection rate was 18%. The age was a risk factor for infection where there was a significant difference in infection rate between sheep more than 3 years and sheep under 3 years of age. There was no significant difference between infection in male and female groups. The liver had the highest organ distribution followed by omentum. Biochemical analysis of the cyst fluid showed some variations in the levels of ALT, AST, triglycerides, cholesterol, total protein, urea nitrogen, calcium, sodium, chromium, potassium than the levels identified in Algeria, Iraq, and Iran. PCR and sequence analysis of cox1 and ssrRNA showed that the sequences from Minoufiya, Egypt were highly identical to the related ones from several countries and confirmed the cyst is Cysticercus tenuicollis. This study reported the infection rate, risk factors, biochemical analysis, and molecular characterization of Cysticercus tenuicollis in sheep from Minoufiya, Egypt.
Assuntos
Cisticercose/veterinária , Cysticercus/isolamento & purificação , Doenças dos Ovinos/epidemiologia , Fatores Etários , Animais , Cisticercose/epidemiologia , Cisticercose/parasitologia , Cysticercus/classificação , Cysticercus/genética , Egito/epidemiologia , Prevalência , Fatores de Risco , Ovinos , Doenças dos Ovinos/parasitologia , Carneiro DomésticoRESUMO
Trichomonas gallinae infects the upper digestive tract of pigeons. It is transmitted from mother to young squabs by feeding crop milk. Generally, infection resulted in severe mortalities in young birds. In this study, we examined 3315 pigeons of different ages from the Minoufiya governorate for the clinical infection by T. gallinae. The infection was confirmed in infected birds by microscopical examination of oral swabs, histopathological examination, and PCR of the ITS1/5.8S/ITS2 gene. The prevalence was 63 (1.9%). The parasite was found in 35 (2.04%) from Ashmoun, 15 (1.66%) from Minoof, 8 (1.6%) from Quesna, and 5 (2.5%) from El-Shohada birds. The infection was mainly detected in squabs 60 (1.8%). The sequence of T. gallinae ITS1/5.8S/ITS2 gene from Egypt has high nucleotide sequence identity (up to100%) to T. gallinae from pigeon of USA, Austria, Canada, and Spain. The sequence belongs to genotype B of T. gallinae. Histopathological examination presented the parasites in crop, liver, larynx, and trachea as poorly eosinophilic bodies with severe inflammatory cell infiltration. This is the first study to present the prevalence and genotype of T. gallinae from Minoufiya governorate, Egypt.