RESUMO
The reproductive toxicity of the insecticide methamidophos was studied in male mice. Adult male mice were treated by gavage with methamidophos at doses of 0, 1, 2 and 3 mg kg(-1) day(-1) for 4 weeks before mating with untreated females. Brain and skeletal muscle acetylcholinesterase activity was inhibited in the middle- and high-treated groups. Methamidophos treatment was associated with a decreased number of live foetuses and an increased number of dead and resorption foetuses at 2 and 3 mg kg(-1) day(-1) treated groups. The per cent morphologically normal spermatozoa were affected in the 2 and 3 mg kg(-1) day(-1) dose groups; however, sperm motility and count were decreased in the same treated groups compared to the control. Histological examination of brain, muscles, testes and epididymis revealed histological abnormalities in a dose-dependent manner. The current study demonstrated adverse effects of male methamidophos exposure on pregnancy outcome with effects on sperm parameters at 2 and 3 mg kg(-1) day(-1) .
Assuntos
Inseticidas/toxicidade , Compostos Organotiofosforados/toxicidade , Resultado da Gravidez , Reprodução/efeitos dos fármacos , Acetilcolinesterase/efeitos dos fármacos , Animais , Ingestão de Alimentos/efeitos dos fármacos , Epididimo/efeitos dos fármacos , Feminino , Morte Fetal/induzido quimicamente , Masculino , Camundongos , Camundongos Endogâmicos ICR , Gravidez , Contagem de Espermatozoides , Motilidade dos Espermatozoides/efeitos dos fármacos , Espermatozoides/efeitos dos fármacosRESUMO
The reproductive toxicity of the insecticide chloropyrifos was studied in male mice. Adult male mice were treated by gavage with chloropyrifos at doses of 0, 5, 15, and 25mg/kg-d for 4 weeks before mating with untreated females. Signs of cholinergic effects were observed in the 15 and 25mg/kg-d treated groups. Brain and skeletal muscle acetylcholinesterase activity was inhibited in the same groups. Chloropyrifos treatment was associated with a decreased number of live fetuses, and an increased number of dead fetuses at 25mg/kg-d. The number of early resorption was decreased in both 15 and 25mg/kg-d treated groups. The percent morphologically normal spermatozoa were affected in 15 and 25mg/kg-d dose groups; however, sperm motility and count were decreased in the same treated groups compared to the control. Histologic examination of brain revealed histological abnormalities in the middle and high groups. Dose related histologic changes, including degeneration of muscle fibers, were observed in the muscles of male mice treated with all the doses of chloropyrifos. The current study demonstrated adverse effects of male chloropyrifos exposure on pregnancy outcome with effects on sperm parameters at 15 and 25mg/kg-d.
