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1.
PLoS One ; 11(10): e0162578, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27706151

RESUMO

A novel mechanism of rifampicin (Rif) resistance has recently been reported in Nocardia farcinica. This new mechanism involves the activity of rifampicin monooxygenase (RifMO), a flavin-dependent monooxygenase that catalyzes the hydroxylation of Rif, which is the first step in the degradation pathway. Recombinant RifMO was overexpressed and purified for biochemical analysis. Kinetic characterization revealed that Rif binding is necessary for effective FAD reduction. RifMO exhibits only a 3-fold coenzyme preference for NADPH over NADH. RifMO catalyzes the incorporation of a single oxygen atom forming an unstable intermediate that eventually is converted to 2'-N-hydroxy-4-oxo-Rif. Stable C4a-hydroperoxyflavin was not detected by rapid kinetics methods, which is consistent with only 30% of the activated oxygen leading to product formation. These findings represent the first reported detailed biochemical characterization of a flavin-monooxygenase involved in antibiotic resistance.


Assuntos
Nocardia/enzimologia , Rifampina/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Biocatálise , Cromatografia Líquida de Alta Pressão , Flavina-Adenina Dinucleotídeo/química , Flavina-Adenina Dinucleotídeo/metabolismo , Cinética , NADP/química , NADP/metabolismo , Nocardia/efeitos dos fármacos , Oxirredução , Oxigênio/química , Oxigênio/metabolismo , Oxigenases/genética , Oxigenases/metabolismo , Ligação Proteica , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/isolamento & purificação , Rifampina/análise , Rifampina/química , Rifampina/farmacologia
2.
Arch Biochem Biophys ; 606: 180-8, 2016 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-27503802

RESUMO

l-lysine (l-Lys) N(6)-monooxygenase (NbtG), from Nocardia farcinica, is a flavin-dependent enzyme that catalyzes the hydroxylation of l-Lys in the presence of oxygen and NAD(P)H in the biosynthetic pathway of the siderophore nocobactin. NbtG displays only a 3-fold preference for NADPH over NADH, different from well-characterized related enzymes, which are highly selective for NADPH. The structure of NbtG with bound NAD(P)(+) or l-Lys is currently not available. Herein, we present a mutagenesis study targeting M239, R301, and E216. These amino acids are conserved and located in either the NAD(P)H binding domain or the l-Lys binding pocket. M239R resulted in high production of hydrogen peroxide and little hydroxylation with no change in coenzyme selectivity. R301A caused a 300-fold decrease on kcat/Km value with NADPH but no change with NADH. E216Q increased the Km value for l-Lys by 30-fold with very little change on the kcat value or in the binding of NAD(P)H. These results suggest that R301 plays a major role in NADPH selectivity by interacting with the 2'-phosphate of the adenine-ribose moiety of NADPH, while E216 plays a role in l-Lys binding.


Assuntos
Proteínas de Bactérias/química , Lisina/química , Oxigenases de Função Mista/química , NADP/química , Nocardia/enzimologia , Aminoácidos/química , Catálise , Flavinas/química , Peróxido de Hidrogênio/química , Hidroxilação , Cinética , Conformação Molecular , Mutação , NAD/metabolismo , Oxigênio/química , Ligação Proteica
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