RESUMO
Polarographic and colorimetric methods for analysis of cyclophosphamide and its dosage forms were investigated. Both methods are based on the reaction of cyclophosphamide with nitrous acid. A single cathodic diffusion-controlled wave was used for dc polarographic determination of cyclophosphamide, with an accuracy of 99.98 +/- 1.09%. The wave was well defined and irreversible. By differential pulse polarographic analysis, as little as 10 ppm cyclophosphamide was determined; overall accuracy at 10-60 ppm was 100.16 +/- 0.99%. The linear relationship between absorbance for the lemon-yellow nitroso derivative and the concentration of cyclophosphamide was further used in colorimetric analysis; overall accuracy was 100.2 +/- 0.99%.
Assuntos
Ciclofosfamida/análise , Fenômenos Químicos , Química , Colorimetria/métodos , Injeções , Polarografia/métodos , Soluções/análise , Comprimidos/análiseRESUMO
The polarographic behaviour of the widely used anti-inflammatory agent, oxyphenbutazone, was studied. It is determined polarographically by conversion to the nitroso derivative characterized by a cathodic, irreversible, diffusion-controlled wave. The method is applied to the determination of 2.5-10 mg/100 mL of oxyphenbutazone, with an accuracy of 99.9 +/- 1.38%. By differential pulse polarographic analysis, as little as 10 ppm oxyphenbutazone can be determined with an accuracy of 99.70 +/- 0.99% in pure powder and in some pharmaceutical formulations.
Assuntos
Oxifenilbutazona/análise , Cápsulas/análise , Polarografia/métodos , Supositórios/análise , Comprimidos/análiseRESUMO
Olive oil was converted into methyl esters which were autoxidized at 60 degrees C. The composition of oxidized products was determined by the comparison of infrared spectra and NMR spectra of the original and acetylated samples, the sample reduced with potassium iodide and the acetylated reduced sample. Oxidized products were separated by preparative thin layer chromatography on silica gel and characterized by selective detection and by infrared spectrometry of the fractions. The oxidation products consisted of hydroperoxido butyl oleate, substituted hydroperoxides, mono- and disubstituted monomeric derivatives and a small amount of oligomers.
