Studies on the effects of aspartame on memory and oxidative stress in brain of mice.

OBJECTIVE: The dipeptide aspartame (N-L-alpha-aspartyl-Lphenylalanine, 1-methyl ester; alpha-APM) is one of the most widely used artificial sweeteners. The present study aimed to investigate the effect of repeated administration of aspartame in the working memory version of Morris water maze test, on oxidative stress and brain monoamines in brain of mice. MATERIALS AND METHODS: Aspartame (0.625, 1.875 or 5.625 mg/kg) was administered once daily subcutaneously for 2 weeks and mice were examined four times a week for their ability to locate a submerged plate. Malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide levels (the concentrations of nitrite/nitrate) and glucose were determined in brain. RESULTS: Only at the highest dose of 5.625 mg/kg, did aspartame significantly impaired water maze performance. The mean time taken to find the escape platform (latency) over 2 weeks was significantly delayed by aspartame 5.625 mg/kg, compared with the saline-treated control group. Significant differences occurred only on the first trial to find the escape platform. Significant increase in brain MDA by 16.5% and nitric oxide by 16.2% and a decrease in GSH by 25.1% and glucose by 22.5% occurred after treatment with aspartame at 1.875 mg/kg. Aspartame administered at 5.625 mg/kg significantly increased brain MDA by 43.8%, nitric oxide by 18.6% and decreased GSH by 32.7% and glucose by 25.8%. Aspartame caused dose-dependent inhibition of brain serotonin, noradrenaline and dopamine. CONCLUSIONS: These findings suggest impaired memory performance and increased brain oxidative stress by repeated aspartame administration. The impaired memory performance is likely to involve increased oxidative stress as well as decreased brain glucose availability.

Interrelationships between anemia and fertility patterns in rural Egyptian women.

PIP: This study investigates the relation of malnutrition as indexed by hemoglobin levels and anthropometry and the fertility of Egyptian rural women. 357 married women in 2 villages in Beheira governorate, aged 15-45 were interviewed. 40.9% were in the optimum childbearing age (20-30 years); mean age was 30.5 years. Mean duration of married life was 10.96 years. The frequency distribution curve of hemoglobin of the group was shifted to the left, indicating a high prevalence of anemia. Mean body weight ranged between 62-64 kg, mean height between 158-63 cm, and mean body index between 2.31-2.85. Weight percentiles and degree of anemia shared a bimodal distribution, while the weight for height percentiles and degree of anemia showed a normal distribution with a shift to the left indicating prevalence of underweight for height. The group had an average of 3.6 previous pregnancies; the highest average was 6.7 attained at the age of 40-45 years. Mean pregnancy interval was 2.14 years; number of live births was an average of 3.27; abortion experiences on the average was 0.22; mean stillbirths was 0.07; highest average child losses was for ages 40-45, 0.75; average number of living children 2.8; and mean reproductive activity increased with increase in years of married life. Anemia tends to reduce fertility at all ages with the mean parity higher in the total normal group (3.44) than in the anemic (3.27). Mean family size was also higher in the normal (3.18) than anemic (2.7) groups. In anemic women percentages of live births was 97.7% compared with 94.4% in the normal group, pregnancy wastage was 10.1% compared to 4% in the normal group and total child losses was 11.3% compared to 10.8% in the normal group. The differences are not considered to be statistically significant. The data indicated that interaction between nutritional states, environmental conditions, and fertility performance is the major determinant of human reproduction.^ieng